Correlation and causation between the microbiome, Wolbachia and host functional traits in natural populations of drosophilid flies

Molecular Ecology, Год журнала: 2019, Номер 28(7), С. 1826 - 1841

Опубликована: Фев. 4, 2019

Abstract Resident microorganisms are known to influence the fitness and traits of animals under controlled laboratory conditions, but relevance these findings wild is uncertain. This study investigated host functional correlates microbiota composition in a community three sympatric species mycophagous drosophilid flies, Drosophila falleni , neotestacea putrida . Specifically, we quantified bacterial communities transcriptomes by parallel 16S rRNA gene amplicon sequencing RNA‐Seq individual flies. Among‐fly variation did not partition strongly sex or species, included multiple modules, that is, sets taxa whose abundance varied concert across different The bacteria several modules significantly with transcripts, especially females, identity correlated transcriptional functions differed including epithelial barrier function D. muscle insect growth development In neotestacea, which harbours endosymbionts Wolbachia Spiroplasma promotes Spiroplasma, positively Lactobacillales Bacteroidales. Furthermore, most correlations between expression relative were co‐correlated (but ), indicative an interdependence traits, this species. These data suggest that, natural populations interact microbial functionally ways can vary endosymbionts.

Язык: Английский

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Microbiome Structure of a Wild Drosophila Community along Tropical Elevational Gradients and Comparison to Laboratory Lines

Applied and Environmental Microbiology, Год журнала: 2023, Номер 89(5)

Опубликована: Май 8, 2023

Bacteria form microbial communities inside most higher-level organisms, but we know little about how the microbiome varies along environmental gradients and between natural host populations laboratory colonies. To explore such effects on insect-associated microbiomes, studied gut in four Drosophila species over two mountain tropical Australia.

Язык: Английский

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Host genetics and larval host plant modulate microbiome structure and evolution underlying the intimate insect–microbe–plant interactions in Parnassius species on the Qinghai‐Tibet Plateau

Ecology and Evolution, Год журнала: 2024, Номер 14(4)

Опубликована: Апрель 1, 2024

Insects harbor a remarkable diversity of gut microbiomes critical for host survival, health, and fitness, but the mechanism this structured symbiotic community remains poorly known, especially insect group consisting many closely related species that inhabit Qinghai-Tibet Plateau. Here, we firstly analyzed population-level 16S rRNA microbial dataset, comprising 11

Язык: Английский

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Organ-specific microbiomes in naturalLotus corniculatuspopulations: Metacommunity dynamics in the plant endosphere

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

ABSTRACT The structure of plant microbial communities vary due to a broad range factors such as host and environmental factors, abiotic biotic perturbations, various assembly processes occurring at multiple tempo-spatial scales. In natural environments are constantly exposed perturbations processes. Thus, attain systemic understanding the ecology microbiomes, it is essential study that influence patterns community structures in environments. this we examined bacterial, fungal, eukaryotic organs Lotus corniculatus populations seven grassland sites for four years. We used framework metacommunity theory understand shape variations by defining associated with roots, shoots, flowers, seeds distinct linked dispersal. show organ-specificity endophytic communities. Our findings suggest selective filtering organs, interactions, well scales result core microbiomes organs. addition, transmission microorganisms from within outside hosts accounts yet overlapping organ microbiomes. could provide comprehensive knowledge stochastic deterministic conditions. Understanding these ecological harnessing beneficial effects plant-associated on productivity, resilience, pathogen defense.

Язык: Английский

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Microbiota acquisition and transmission in Drosophila flies

iScience, Год журнала: 2023, Номер 26(9), С. 107656 - 107656

Опубликована: Авг. 17, 2023

Understanding the ecological and evolutionary dynamics of host-microbiota associations notably involves exploring how members microbiota assemble whether they are transmitted along host generations. Here, we investigate larval acquisition facultative bacterial yeast symbionts Drosophila melanogaster suzukii in ecologically realistic setups. Fly mothers fruit were major sources symbionts. Microorganisms associated with adult males also contributed to microbiota, mostly D. melanogaster. Yeasts acquired at stage maintained through metamorphosis, life, offspring. All these observations varied widely among microbial strains, suggesting have different transmission strategies fruits insects. Our approach shows insects can be from a diversity highlights compound nature microbiotas. Such events generations should favor evolution mutualistic interactions enable microbiota-mediated local adaptation insect host.

Язык: Английский

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Skin bacterial metacommunities of San Francisco Bay Area salamanders are structured by host genus and habitat quality

FEMS Microbiology Ecology, Год журнала: 2021, Номер 97(12)

Опубликована: Дек. 1, 2021

ABSTRACT Host-associated microbial communities can influence physiological processes of macroorganisms, including contributing to infectious disease resistance. For instance, some bacteria that live on amphibian skin produce antifungal compounds inhibit two lethal fungal pathogens, Batrachochytrium dendrobatidis (Bd) and salamandrivorans (Bsal). Therefore, differences in microbiome composition among host species or populations within a contribute variation susceptibility Bd/Bsal. This study applies 16S rRNA sequencing characterize the bacterial microbiomes three widespread terrestrial salamander genera native western United States. Using metacommunity structure analysis, we identified dispersal barriers for these influential between families localities. We also analysed effects habitat characteristics such as percent natural cover temperature seasonality microbiome. found certain environmental variables may more strongly than others. Each family had somewhat distinct community putative anti-Bd bacteria, suggesting salamanders select functional assembly cutaneous symbionts could differ its ability protect amphibians from disease. Our observations raise need consider identity heterogeneity during selection probiotics treat wildlife diseases.

Язык: Английский

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Environmental heterogeneity as a driver of terrestrial biodiversity on a global scale

Progress in Physical Geography Earth and Environment, Год журнала: 2023, Номер 47(6), С. 912 - 930

Опубликована: Авг. 11, 2023

To improve the effectiveness of biodiversity conservation and risk assessments under global changes, it is necessary to understand drivers terrestrial on a scale. Environmental heterogeneity an important umbrella term for different environmental factors that contribute species diversity. Previous studies have shown there are significant relationships between geodiversity scale, in features variables, indicators (EH), drive at local regional scales. However, we do not yet know how maintained, well represented taxa, where would they be more risks considering their abundances diversities. In this study, quantified EH climate, topography, land cover. We used four theoretical indexes (i.e., Fisher’s alpha, Shannon’s H, Hurlbert’s PIE, Good’s u) quantify based abundance regression models explore across organismic groups (ants, bats, birds, butterflies, frogs, ground beetles, mosquitoes, odonates, orthopterans, rodents, scarab trees) globally. found biodiversity, particularly trees three components (climate, cover), however, effects may vary among organisms. Land cover could affect ants, beetles. Furthermore, were topographic trees. Climatic had all organism groups. Our study provides new insights into by EH, indicator geodiversity.

Язык: Английский

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Microhabitat Governs the Microbiota of the Pinewood Nematode and Its Vector Beetle: Implication for the Prevalence of Pine Wilt Disease

Microbiology Spectrum, Год журнала: 2022, Номер 10(4)

Опубликована: Июнь 27, 2022

Our understanding of environmental acquisition microbes and migration-related alteration microbiota across habitats has rapidly increased. However, in complex life cycles, such as for many parasites, exactly how these are transmitted multiple environments, hosts habitats, is unknown. Pinewood nematode, the causal agent globally devastating pine wilt disease, provides an ideal model to study role multispecies interactions because its successful host invasion depends on among vector insects, hosts, associated microbes. Here, we studied bacterial fungal communities involved nematode's cycle different micro- (pupal chamber, beetle, dispersal nematodes) macrohabitats (geographical locations). We identified potential sources, selection processes, keystone taxa pine-nematode-vector beetle interactions. Nearly 50% tracheae ~60% that third-stage juveniles were derived from chambers), whereas 90% bacteria fourth-stage originated tracheae. results also suggest beetles' selectively acquire some key microbial community pupal chambers. These will be then enriched nematodes traveling hence likely transported new trees. Taken together, our findings contribute critical information toward a better therefore aiding knowledge development future biological control agents. IMPORTANCE animal dispersal-mediated variation In this study, using pine-pinewood nematode-vector (Monochamus sp.) complex, disentangled routes assembly transmission mechanisms participants responsible highly destructive disease. provide evidence microhabitat driving force shaping participants. The (LIII) collected around chambers beetles mainly vector-entering (LIV) had simplest community, not influencing vector's microbiota. enhanced plants animals shed light cycles.

Язык: Английский

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Disentangling direct from indirect effects of habitat disturbance on multiple components of biodiversity

Journal of Animal Ecology, Год журнала: 2022, Номер 91(11), С. 2220 - 2234

Опубликована: Авг. 17, 2022

Human habitat disturbance affects both species diversity and intraspecific genetic diversity, leading to correlations between these two components of biodiversity (termed species-genetic correlation, SGDC). However, whether SGDC predictions extend host-associated communities, such as the intestinal parasite gut microbial remains largely unexplored. Additionally, role dominant generalist is often neglected despite their importance in shaping environment experienced by other members ecological community, source, reservoir vector zoonotic diseases. New analytical approaches (e.g. structural equation modelling, SEM) can be used assess relationships distinguish among direct indirect effects characteristics on various biodiversity. With six concrete biologically sound models mind, we collected 22 study sites from four distinct landscapes located central Panama. Each landscape differed degree human fragmentation measured several quantitative variables, canopy cover, height understorey density. In terms biodiversity, estimated one hand, (a) small mammal and, (b) genome-wide (c) (d) heterogeneity most (Tome's spiny rat, Proechimys semispinosus). We SEMs links biological measures. The best supported SEM suggested that directly positively affect richness mammals, P. semispinosus its heterogeneity. Habitat did not, however, impact diversity. also detected indirect, positive assemblages via richness. For microbes, this likely linked cross transmission, particularly shared and/or anthropogenically altered habitats, whereas host mitigates infections. revealed an additional but negative effect Our showcases alterations not only parallel, assemblages. impacts are therefore expected ripple through entire ecosystems with far reaching felt even species.Las perturbaciones antropogénicas sobre los hábitats naturales pueden afectar tanto a la diversidad de las especies como genética intraespecífica, dando lugar correlaciones entre estos dos elementos biodiversidad (denominados correlación especies, por sus siglas en inglés). Sin embargo, todavía queda sin explorar si predicciones afectan comunidades parásitos y microorganismos intestinales asociadas al hospedador. Adicionalmente, el rol que juegan generalistas, especialmente aquéllas dominantes, suele ser descuidado, pesar importancia control ejercen estructura comunidad, su fuente, reservorio enfermedades zoonóticas. Para poder evaluar relaciones distinguir efectos directos e indirectos tienen características del hábitat distintos componentes biodiversidad, se utilizar nuevos enfoques analíticos ejemplo modelos ecuaciones estructurales (SEM, Considerando seis específicos biológicamente sólidos, recopilamos sitios ubicados cuatro paisajes situados centro Panamá. Cada paisaje difería grado perturbación antropogénica fragmentación, medido diferentes variables cuantitativas, cobertura dosel, altura dosel densidad sotobosque. En términos un lado estimamos (1) pequeños mamíferos y, otro (2) genoma completo, (3) intestinales, (4) heterogeneidad microbianas intestino especie generalista más dominante, rata espinosa Tomes semispinosus. vínculos medidas biológica utilizó modelado SEM. El mejor apoyado sugirió directa positivamente abundancia mamíferos, microbiana intestinal. observó no efecto directo intestinales. Aparte directos, detectamos positivos ambos conjuntos asociados hospedador (diversidad intestinales) través mamíferos. caso microbianas, esto está probablemente relacionado con transmisión interespecífica, compartidos y/o antropogénicamente alterados; mientras hospedadores mitiga infecciones parásitos. reveló indirecto adicional pero negativo hospedadores. Nuestro estudio muestra patrones filtran varias capas biológica, añadiendo ensamblajes biológicos afectados alteraciones hábitat.

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The presence of Pseudogymnoascus destructans, a fungal pathogen of bats, correlates with changes in microbial metacommunity structure

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Июнь 3, 2021

Abstract Metacommunity theory provides a framework for how community patterns arise from processes across scales, which is relevant understanding in host-associated microbial assemblages. Microbial metacommunities may have important roles host health through interactions with pathogens; however, it unclear pathogens affect metacommunities. Here, we studied relationships between fungal pathogen and metacommunity. We hypothesized that of bats, Pseudogymnoascus destructans, correlates shift metacommunity structure changes composition, factors shaping these assemblages, such as ecoregion. sampled bat cutaneous assemblages the presence/absence P. destructans analyzed composition structuring variables. Absence correlated characterized by common core group was lacking disease positive bats. Additionally, presence change relationship Our results suggest intensifies local influencing highlights importance host–pathogen interactions.

Язык: Английский

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