Integrated Analysis of Metatranscriptome and Amplicon Sequencing to Reveal Distinctive Rhizospheric Microorganisms of Salt-Tolerant Rice DOI Creative Commons

Wenna Meng,

Zhou ZhenLing,

Mingpu Tan

и другие.

Plants, Год журнала: 2024, Номер 14(1), С. 36 - 36

Опубликована: Дек. 26, 2024

Salt stress poses a significant constraint on rice production, so further exploration is imperative to elucidate the intricate molecular mechanisms governing salt tolerance in rice. By manipulating rhizosphere microbial communities or targeting specific functions, it possible enhance crops, improving crop yields and food security saline environments. In this study, we conducted rhizospheric amplicon sequencing metatranscriptome analysis, revealing substantial microbiomic differences between salt-tolerant cultivar TLJIAN salt-sensitive HUAJING. Fungal taxa including Hormiactis, Emericellopsis, Ceriosporopsis, Dirkmeia, Moesziomyces predominated of rice, while bacterial genera such as Desulfoprunum Hydrogenophaga exhibited notable differences. Metatranscriptomic analysis identified 7192 differentially expressed genes (DEGs) two varieties, with 3934 being upregulated 3258 downregulated. Enrichment analyses KEGG GO pathways highlighted majority DEGs were associated “two-component system”, “sulfur metabolism”, “microbial metabolism diverse environments”. The interaction network revealed upregulation transporters, transcriptional factors, chaperones, ABC transporters chaperonin GroEL, microbiomes varieties. Our multi-omics unveiled that fungi like Ceriosporopsis Dirkmeria, along bacteria Desulfoprunum, Rippkaea, Bellilinea, showed positive correlation flavonoid synthesis This study provides an in-depth distinctive shedding light complex interactions these consortia their host plants under conditions.

Язык: Английский

A comprehensive review on rice responses and tolerance to salt stress DOI Creative Commons

Obed Kweku Sackey,

Naijie Feng, Y A Mohammed

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 31, 2025

The challenge of salinity stress significantly impacts global rice production, especially in coastal and arid regions where the salinization agricultural soils is on rise. This review explores complex physiological, biochemical, genetic mechanisms contributing to tolerance (Oryza sativa L.) while examining agronomic multidisciplinary strategies bolster resilience. Essential adaptations encompass regulation ionic balance, management antioxidants, adjustments osmotic pressure, all driven by genes such as OsHKT1;5 transcription factors like OsbZIP73. evolution breeding strategies, encompassing traditional methods cutting-edge innovations, has produced remarkable salt-tolerant varieties FL478 BRRI dhan47. advancements this field are enhanced including integrated soil management, crop rotation, chemical treatments spermidine, which through antioxidant activity transcriptional mechanisms. Case studies from South Asia, Sub-Saharan Africa, Middle East and, Australia demonstrate transformative potential utilizing varieties; however, challenges persist, polygenic nature tolerance, environmental variability, socioeconomic barriers. highlights importance collaborative efforts across various disciplines, merging genomic technologies, sophisticated phenotyping, inclusive practices foster climate-resilient sustainable cultivation. work seeks navigate complexities its implications for food security, employing inventive cohesive confront posed climate change.

Язык: Английский

Процитировано

0
The Effect of Climate Variables, Soil Characteristics, and Peanut Cultivars on the Rhizobial Bacteria Community DOI Creative Commons
Juan Li,

Zhong-yi Yang,

En Tao Wang

и другие.

Microorganisms, Год журнала: 2025, Номер 13(4), С. 926 - 926

Опубликована: Апрель 17, 2025

Peanuts are widely cultivated across the world; however, peanut’s rhizobial community and determinant factors of their composition still to be elucidated. This study investigates biogeography soil environmental for peanut rhizobia. A total 1001 isolates were obtained from root nodules, mainly belonging two cultivars (X9 M6) in 20 sampling sites China. According recA sequence analysis, all classified as 84 haplotypes, a representative strain each haplotype was randomly selected perform subsequent analyses. Based on multilocus analysis (MLSA) housekeeping genes dnaK, glnII, gyrB, recA, rpoB, strains 42 genospecies genus Bradyrhizobium, including 12 effectively published 30 undefined genospecies. Strains six predominant (>5%), B. ottawaense, liaoningense, yuanmingense, Bradyrhizobium sp. XXIX, guangdongense, nanningense. However, only single isolate 15 The diversity indices rhizobia distributed South China obviously higher than those North China, but no obvious cultivar selection found. Correlation analyses indicated that affected by MAP, MAT, AP, pH. Nodulation tests 79 37 with both nodC nifH could nitrogen-fixing symbiosis peanuts. revealed great varied communities different geographic regions

Язык: Английский

Процитировано

0
Integrated Analysis of Metatranscriptome and Amplicon Sequencing to Reveal Distinctive Rhizospheric Microorganisms of Salt-Tolerant Rice DOI Creative Commons

Wenna Meng,

Zhou ZhenLing,

Mingpu Tan

и другие.

Plants, Год журнала: 2024, Номер 14(1), С. 36 - 36

Опубликована: Дек. 26, 2024

Salt stress poses a significant constraint on rice production, so further exploration is imperative to elucidate the intricate molecular mechanisms governing salt tolerance in rice. By manipulating rhizosphere microbial communities or targeting specific functions, it possible enhance crops, improving crop yields and food security saline environments. In this study, we conducted rhizospheric amplicon sequencing metatranscriptome analysis, revealing substantial microbiomic differences between salt-tolerant cultivar TLJIAN salt-sensitive HUAJING. Fungal taxa including Hormiactis, Emericellopsis, Ceriosporopsis, Dirkmeia, Moesziomyces predominated of rice, while bacterial genera such as Desulfoprunum Hydrogenophaga exhibited notable differences. Metatranscriptomic analysis identified 7192 differentially expressed genes (DEGs) two varieties, with 3934 being upregulated 3258 downregulated. Enrichment analyses KEGG GO pathways highlighted majority DEGs were associated “two-component system”, “sulfur metabolism”, “microbial metabolism diverse environments”. The interaction network revealed upregulation transporters, transcriptional factors, chaperones, ABC transporters chaperonin GroEL, microbiomes varieties. Our multi-omics unveiled that fungi like Ceriosporopsis Dirkmeria, along bacteria Desulfoprunum, Rippkaea, Bellilinea, showed positive correlation flavonoid synthesis This study provides an in-depth distinctive shedding light complex interactions these consortia their host plants under conditions.

Язык: Английский

Процитировано

1