Comparative Analysis of Water Isotopic Compositions: Evaluating Isotope Analyzer for Soil and Extraction Method for Stem Water

Forests, Год журнала: 2024, Номер 15(3), С. 420 - 420

Опубликована: Фев. 22, 2024

Stable isotopes of water (δ2H and δ18O) are reliable tracers for the investigation plant–soil–water interactions in forest ecosystems. However, variations isotopic compositions may arise due to differences analytical instruments extraction methods. In this study, we conducted three different experiments identify caused by methodological variations. First, analyzed soil using two most commonly applied methods: isotope ratio mass spectrometry (IRMS) cavity ring-down spectroscopy (CRDS). Second, compared xylem extracted from stems nine tree species cryogenic vacuum distillation (CVD) with heating times. Third, mechanical squeezing a pressure chamber (PC), an induction module (IM), CVD. The composition between IRMS CRDS were significant but minimal. Soil properties not factors contributing instruments. For CVD, each required more than hours. Significant observed δ2H among methods water. Xylem CVD showed depleted values those obtained PC IM. Our results highlight importance considering stable analysis.

Язык: Английский

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UAV-based land surface temperatures and vegetation indices explain and predict spatial patterns of soil water isotopes in a tropical dry forest

Authorea (Authorea), Год журнала: 2024, Номер unknown

Опубликована: Март 5, 2024

The spatial variation of soil water isotopes (SWI) - representing the baseline for investigating root uptake (RWU) depths with stable isotope techniques has rarely been investigated. Here, we use SWI depth profile sampling in combination unmanned aerial vehicle (UAV) based land surface temperature estimates and vegetation indices (VI) order to improving process understanding relationships between content patterns canopy status. We carried out a ten profiles tropical dry forest. UAV data were collected analyzed obtain detailed characterization then performed statistical analysis VI temperatures values at different resolutions (3 cm 5 m). Best used generating isoscapes entire study area. Results suggest that are strongly mediated by parameters (VI). Various correlate across all depths. depend on (R² 0.65 δ18O 0.57 δ2H). Strongest overall correlations found resolution 0.5 m. speculate this might be ideal spatially characterizing investigate RWU. Supporting analyses UAV-based approaches future avenue representation credibility such studies.

Язык: Английский

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Cavitron extraction of xylem water suggests cryogenic extraction biases vary across species but are independent of tree water stress

Authorea (Authorea), Год журнала: 2023, Номер unknown

Опубликована: Окт. 30, 2023

Cryogenic vacuum distillation (CVD) is a widely used technique for extracting plant water from stems isotopic analysis, but concerns about potential biases have emerged. Here, we leverage the Cavitron centrifugation to extract xylem and compare its signature that of CVD-extracted stem as well source water. Conducted under field conditions in tropical northern Australia, our study spans seven tree species naturally experiencing range stress levels. Our findings reveal significant deuterium bias bulk when compared (median –14.9‰), whereas closely aligned with offset –1.9‰). We find substantial variations among (bias ranging –19.3 –9.1‰), intriguingly, CVD-induced were unrelated environmental factors such relative content pre-dawn leaf potential. These results imply inter-specific differences may be driven by anatomical traits rather than hydraulic functioning. Additionally, data highlight use site-specific offset, based on local water, correcting biases.

Язык: Английский

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Cavitron extraction of xylem water suggests cryogenic extraction biases vary across species but are independent of tree water stress

Authorea (Authorea), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 15, 2023

Cryogenic vacuum distillation (CVD) is a widely used technique for extracting plant water from stems isotopic analysis, but concerns about potential biases have emerged. Here, we leverage the Cavitron centrifugation to extract xylem and compare its signature that of CVD-extracted stem as well source water. Conducted under field conditions in tropical northern Australia, our study spans seven tree species naturally experiencing range stress levels. Our findings reveal significant deuterium bias bulk when compared (median -14.9‰), whereas closely aligned with offset -1.9‰). We find substantial variations among (bias ranging -19.3 -9.1‰), intriguingly, CVD-induced were unrelated environmental factors such relative content pre-dawn leaf potential. These results imply inter-specific differences may be driven by anatomical traits rather than hydraulic functioning. Additionally, data highlight use site-specific offset, based on local water, correcting biases.

Язык: Английский

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