When Methodological Innovation Changes the Game: A 10‐Year Review of Environmental DNA (eDNA) Applied to Crayfish DOI Creative Commons
Thomas Baudry, Valentin Vasselon,

Carine Delaunay

и другие.

Aquatic Conservation Marine and Freshwater Ecosystems, Год журнала: 2024, Номер 34(9)

Опубликована: Авг. 30, 2024

ABSTRACT The use of environmental DNA (eDNA) as a tool for monitoring represents major innovative advance in science, one that enables the detection species without need to observe or capture them. This article assesses state play eDNA research targeting crayfish. We found total 41 peer‐reviewed articles published between 2014 and 2023 on both native invasive species. Most studies focused (or native/invasive co‐detection assessment) (65.8%). There was also clear geographical bias across studies, with more than half conducted Europe (51.2%) quarter United States (26.8%). In contrast, there were none Africa. relatively large number has led an interesting diversity protocols designed utilized, most favouring development their own assays (69.33%). That said, filtration (as method) common (80.5%), along commercially available extraction kits (69.8%). COI gene appeared be preferred target region (89.33%). Such range is interesting, but it optimal? Are best always being utilized? Or chance novel application hampering our ability explore larger trends studies?

Язык: Английский

Hydrodynamics and Aquatic Vegetation Drive Spatial Patterns of Environmental DNA in Ponds DOI Creative Commons
Sandra R. Mayne, Jeffrey A. Manning, Stephen M. Henderson

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(6)

Опубликована: Ноя. 1, 2024

ABSTRACT Environmental DNA (eDNA) sampling is a powerful method for detecting aquatic species at low densities. However, eDNA may remain close to the source in lentic systems, decreasing effectiveness of surveys. We conducted cage experiments with salamanders and simultaneous detailed hydrologic wind measurements investigate influence physical environment on detection patterns ponds. found much higher rates surface layer than depth, that vegetation reduced produced open water by 80%–94%. Within mixed layer, were highest direction flow bottom half detections farthest from occurred when velocities this sublayer high. Detections near zero even was flowing fast away point. The lower negatively correlated most study. These spatial temporal dynamics indicate transport processes ponds are highly complex. Sampling vegetation, upwind potential sources, addition many locations within pond considering patterns, improve rare species. This work contributes growing body literature characterizing variability systems.

Язык: Английский

Процитировано

2
Environmental DNA surveys can underestimate amphibian occupancy and overestimate detection probability: implications for practice DOI Creative Commons
Lea A. Randall, Caren S. Goldberg,

Axel Moehenschlager

и другие.

Journal of Wildlife Management, Год журнала: 2023, Номер 87(7)

Опубликована: Июль 5, 2023

Abstract Environmental DNA analysis of water samples has recently emerged as a powerful tool for studying the distribution, ecology, and conservation many amphibian species. Validating efficacy through comparison with established methods detection is important any new method. We used multi‐method single‐method occupancy models to explore effectiveness 3 techniques detecting northern leopard frogs ( Lithobates pipiens ) boreal chorus Pseudacris maculata ). conducted surveys using automated recording units, environmental sampling, visual encounter in spring (30 Apr–3 Jun) summer (23 Jul–27 Aug) 2014 at 68 sites southern Alberta, Canada. The incorporated data from all survey while accounting lack independence detections within sampling occasion, we compared this analyses biases associated each method differences method‐specific probability. Occupancy probabilities estimated were biased frogs, overestimating probability underestimating occupancy. could not assess bias because overdispersion present models. Although no single covariate explained bias, it was ameliorated (albeit by reducing precision) including raw count individuals proxy abundance during breeding but post‐metamorphic season. These results emphasize need careful consideration temporal spatial aspects design, conducting pilot studies, external validation eDNA‐only monitoring schemes prior widespread implementation.

Язык: Английский

Процитировано

5
Contrasting patterns of biodiversity across wetland habitats using single‐time‐point environmental DNA surveys DOI Creative Commons
Harrison T. Coleman, Ty G. Matthews, Craig D. H. Sherman

и другие.

Aquatic Conservation Marine and Freshwater Ecosystems, Год журнала: 2023, Номер 33(12), С. 1401 - 1412

Опубликована: Окт. 30, 2023

Abstract Wildlife surveys are central to the conservation and restoration management of wetland habitats; however, often laborious costly nature traditional survey methods can constrain spatial temporal extent replication efforts. Environmental DNA (eDNA) technologies now provide opportunity reduce some these limitations, but applications in temporary permanent freshwater marshes meadows (herein referred as ‘wetland habitats’) remain limited. This study investigates performance single‐time‐point eDNA for characterizing fish, amphibian bird assemblages habitats south‐eastern Australia. Comparisons were made between coastal inland wetlands, wetlands varying condition surveys. Findings show that comprehensive descriptions faunal associated with habitats, resolution similar provided by approaches. Analyses also revealed significant differences patterns biodiversity types (coastal vs. inland) not condition. Overall, this confirms a reliable option undertaking assessments aimed at improving data coverage otherwise deficient wetlands.

Язык: Английский

Процитировано

5
Development and optimization of a novel environmental DNA‐based method for moor frog (Rana arvalis) monitoring and comparison with a commercial detection kit DOI Creative Commons
Suvi Olli,

Petri Lampila,

Laura Kvist

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(1)

Опубликована: Янв. 1, 2024

Abstract Moor frogs ( Rana arvalis ) are protected by the European Union's Habitats Directive, often making them a target for environmental impact assessments. However, moor frog detection with traditional method using their mating calls is difficult, time‐consuming, and limited to short season. Environmental DNA (eDNA)‐based methods could be beneficial in since shy co‐occurring common R. temporaria morphologically similar. Additionally, eDNA‐based even extend period of frogs. We tested two different isolation eDNA methods, Sanger sequencing quantitative PCR (qPCR), compared results call survey method. In addition, we whether linear polyacrylamide treatment would improve detection. sampled 27 sites Finland, which were detected 17 acoustic method, 20 sequencing‐based 21 qPCR‐based water bodies during 3–4 weeks after end Our show that both (Sanger qPCR) can used detect at least weeks, but qPCR slightly outperforms sequencing. Linear did not results. Detection probabilities similar or higher than as also heard. Based on our results, highly monitoring presence complement surveys.

Язык: Английский

Процитировано

1
When Methodological Innovation Changes the Game: A 10‐Year Review of Environmental DNA (eDNA) Applied to Crayfish DOI Creative Commons
Thomas Baudry, Valentin Vasselon,

Carine Delaunay

и другие.

Aquatic Conservation Marine and Freshwater Ecosystems, Год журнала: 2024, Номер 34(9)

Опубликована: Авг. 30, 2024

ABSTRACT The use of environmental DNA (eDNA) as a tool for monitoring represents major innovative advance in science, one that enables the detection species without need to observe or capture them. This article assesses state play eDNA research targeting crayfish. We found total 41 peer‐reviewed articles published between 2014 and 2023 on both native invasive species. Most studies focused (or native/invasive co‐detection assessment) (65.8%). There was also clear geographical bias across studies, with more than half conducted Europe (51.2%) quarter United States (26.8%). In contrast, there were none Africa. relatively large number has led an interesting diversity protocols designed utilized, most favouring development their own assays (69.33%). That said, filtration (as method) common (80.5%), along commercially available extraction kits (69.8%). COI gene appeared be preferred target region (89.33%). Such range is interesting, but it optimal? Are best always being utilized? Or chance novel application hampering our ability explore larger trends studies?

Язык: Английский

Процитировано

1