eDNA metabarcoding: a non-invasive method to track temporal community dynamics in temporary rivers DOI Creative Commons
Nieves López‐Rodríguez, Dominik Buchner, Miguel Cañedo‐Argüelles

и другие.

Limnetica, Год журнала: 2024, Номер 45(1), С. 1 - 1

Опубликована: Дек. 20, 2024

Temporary rivers (TRs) are dynamic ecosystems that alternate between hydrological phases (i.e. flowing, disconnected pools, and dry). They conservation refugia for aquatic species during dry seasons but often neglected in bioassessment programs. To assess the biological quality of these ecosystems, morphology methods can be invasive, disrupting communities diminishing their function as refugia. Environmental DNA (eDNA) metabarcoding provides a minimally invasive method, gathering community information from eDNA water or sediment. We tested effectiveness alongside bulk to characterize macroinvertebrate pools TRs, comparing them with morphological methods. Additionally, we how patterns evolve over time using composition shifts disconnection. Biological was determined through indices widely used Spain (i.e., IBMWP, family richness, IASPT). samples were collected biweekly three TRs Catalonia, NE Spain. Macroinvertebrates sampled (connected, disconnecting, pools). Macroinvertebrate identify organisms sequence DNA. sequenced targeting mitochondrial COI gene. Although by sediment did not detect variations biotic IBMWP richness), method useful replacement EPT (Ephemeroptera, Plecoptera, Trichoptera) OCH (Odonata, Coleoptera, Heteroptera). revealed significant impacts changes on meiofauna (Ostracoda, Cladocera, Copepoda), group overlooked stream assessments. These results indicate serve valuable tool capturing transitions while preserving ecosystem integrity.

Язык: Английский

Biodiversity Monitoring in Remote Marine Environments: Advancing Environmental DNA/RNA Sampling Workflows DOI Creative Commons
Michelle Scriver, Anastasija Zaiko, Xavier Pochon

и другие.

Marine Environmental Research, Год журнала: 2025, Номер 206, С. 107041 - 107041

Опубликована: Фев. 26, 2025

Язык: Английский

Процитировано

1
The Use of Environmental DNA as Preliminary Description of Invertebrate Diversity in Three Sicilian Lakes DOI Creative Commons
Manuela Mauro, Francesco Longo, Mario Lo Valvo

и другие.

Animals, Год журнала: 2025, Номер 15(3), С. 355 - 355

Опубликована: Янв. 26, 2025

Freshwater ecosystems are among the most severely affected environments by species loss caused climate change and intense anthropogenic pressure. To preserve biodiversity, biomonitoring plays a key role providing reliable data on biological diversity ecological status. Environmental DNA (eDNA) metabarcoding has emerged as powerful non-invasive alternative to traditional morphology-based sampling identification methods. This study represents first application of eDNA analysis assess invertebrate communities in three Sicilian Lakes: Poma, Piana degli Albanesi Scanzano. Water samples were collected at two points each lake after filtration with nitrocellulose membranes, was extracted performed. A total 27 identified, belonging Phyla Annelida, Arthropoda Rotifera. Notably, revealed presence alien (Daphnia parvula Acanthocyclops americanus), dangerous associated transmission viral diseases (Culex pipiens), potential new records for Sicily (Stylaria lacustris, Platypalpus exilis, Pammene aurana, Limnephilus rhombicus). These results provide preliminary snapshot biodiversity these sites, demonstrating how complement, but not replace, methods, contributing assessment ecosystem

Язык: Английский

Процитировано

0
Evaluating the untapped potential of forest landscape restoration to streams DOI
Paula C. dos Reis Oliveira, Solange Filoso, Sílvio Frosini de Barros Ferraz

и другие.

Restoration Ecology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Scientists and forest restoration practitioners generally expect positive outcomes for freshwater ecosystems from restoration, but these expectations may not be met if streams are considered during the planning phase of projects or adequately monitored. In general, decisions on how to improve water quality protect stream health through landscape have been based mostly socio‐economic rationales rather than scientific knowledge about affects key processes that support ecosystem functions, such as nutrient cycling, sediment transport, habitat provision, temperature regulation. As ecologists hydrologists, we see expansion in tropics an opportunity rehabilitate impacted by unprecedented intensification agriculture region. this paper, provide insights into can better planned ecological ecosystems.

Язык: Английский

Процитировано

0
Environmental DNA reveals temporal and spatial variability of invertebrate communities in arid-lands ephemeral water bodies DOI Creative Commons
B. A. Hedges, Perry G. Beasley‐Hall, James B. Dorey

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 3, 2024

Abstract Context Throughout semi-arid and arid Australia surface freshwater is rare, where it does occur, often ephemeral. This the case for granite rock-holes that occur throughout much of southern Australia. Rock-holes support invertebrate communities, however, ongoing threat climate change means this ecosystem likely to experience hydrological disruptions. are also be heavily impacted by invasive vertebrates. However, ecology poorly understood despite its relative ecological significance extent associated threats. Aims methods To provide a baseline understanding we documented species richness variability at series in Gawler bioregion South using an environmental DNA approach. Key results Metabarcoding recorded invertebrates from 22 orders 45 families. Community composition varied among year, with peak winter. Conclusions implications These findings demonstrate importance these ecosystems range endemic taxa. We propose establishment monitoring programs, development custom barcode reference libraries rock-hole future research into impacts on communities them.

Язык: Английский

Процитировано

0
Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem DOI Creative Commons
Michelle Scriver, Ulla von Ammon, Xavier Pochon

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(6)

Опубликована: Ноя. 1, 2024

ABSTRACT Environmental DNA (eDNA) offers an efficient approach to biodiversity monitoring and biosecurity surveillance in coastal ecosystems. Understanding eDNA dynamics tidal areas is critical for guiding sampling strategy design interpreting molecular detection results. In a field study conducted at Ōpua, Bay of Islands, Aotearoa, New Zealand, international marina with dynamic environment, we investigated patterns across local spatial temporal gradient. Hourly samples were collected from six stations around the marina, artificial shore structures channel, over 12‐h cycle. Detected eukaryotic communities assessed using metabarcoding analysis small‐subunit ribosomal RNA (18S rRNA) mitochondrial Cytochrome C Oxidase subunit I (COI) genes. This core community was screened marine nonindigenous species (NIS) online data screening app (ExPAT). Community composition varied significantly based on location time, most captured mid‐tide. The NIS exhibited inconsistent some (e.g., amphipod Monocorophium acherusicum ) space whereas other mussel Arcuatula senhousia consistently detected behaved similar community. Overall, species‐specific may be linked biology movement organism. results indicated slightly higher within 2 h low tide. For applications, users want prioritize near‐shore during tide, while mid‐tide provide more comprehensive insights into biomonitoring. Further studies multiple cycles marinas guide standardization biomonitoring biosurveillance strategies support their broader integration programs.

Язык: Английский

Процитировано

0
eDNA metabarcoding: a non-invasive method to track temporal community dynamics in temporary rivers DOI Creative Commons
Nieves López‐Rodríguez, Dominik Buchner, Miguel Cañedo‐Argüelles

и другие.

Limnetica, Год журнала: 2024, Номер 45(1), С. 1 - 1

Опубликована: Дек. 20, 2024

Temporary rivers (TRs) are dynamic ecosystems that alternate between hydrological phases (i.e. flowing, disconnected pools, and dry). They conservation refugia for aquatic species during dry seasons but often neglected in bioassessment programs. To assess the biological quality of these ecosystems, morphology methods can be invasive, disrupting communities diminishing their function as refugia. Environmental DNA (eDNA) metabarcoding provides a minimally invasive method, gathering community information from eDNA water or sediment. We tested effectiveness alongside bulk to characterize macroinvertebrate pools TRs, comparing them with morphological methods. Additionally, we how patterns evolve over time using composition shifts disconnection. Biological was determined through indices widely used Spain (i.e., IBMWP, family richness, IASPT). samples were collected biweekly three TRs Catalonia, NE Spain. Macroinvertebrates sampled (connected, disconnecting, pools). Macroinvertebrate identify organisms sequence DNA. sequenced targeting mitochondrial COI gene. Although by sediment did not detect variations biotic IBMWP richness), method useful replacement EPT (Ephemeroptera, Plecoptera, Trichoptera) OCH (Odonata, Coleoptera, Heteroptera). revealed significant impacts changes on meiofauna (Ostracoda, Cladocera, Copepoda), group overlooked stream assessments. These results indicate serve valuable tool capturing transitions while preserving ecosystem integrity.

Язык: Английский

Процитировано

0