Revealing the polar lipidome, pigment profiles, and antioxidant activity of the giant unicellular green alga, Acetabularia acetabulum DOI
Felisa Rey, Paulo Cartaxana, Sónia Cruz

и другие.

Journal of Phycology, Год журнала: 2023, Номер 59(5), С. 1025 - 1040

Опубликована: Июль 23, 2023

Marine algae are one of the most important sources high-value compounds such as polar lipids, omega-3 fatty acids, photosynthetic pigments, or secondary metabolites with interesting features for different niche markets. Acetabularia acetabulum is a macroscopic green single-celled alga, single nucleus hosted in rhizoid. This alga studied dasycladalean species and represents an model system cell biology studies. However, its lipidome pigment profile have been overlooked. Total lipid extracts were analyzed using hydrophilic interaction liquid chromatography-high resolution mass spectrometry (HILIC-HRMS), tandem (MS/MS), high-performance chromatography (HPLC). The antioxidant capacity was tested DPPH ABTS assays. Lipidomics identified 16 classes, corresponding to glycolipids, betaine phospholipids, sphingolipids, total 191 species, some them recognized by their bioactivities. abundant lipids glycolipids. Lipid classes less identified, diacylglyceryl-carboxyhydroxymethylcholine (DGCC) hexosylceramide (HexCer). A. comprised carotenoids (17.19%), namely cis-neoxanthin, violaxanthin, lutein β,β-carotene, chlorophylls b (82.81%). showed high activity promoting 50% inhibition (IC50 ) concentrations 57.91 ± 1.20 μg · mL-1 (438.18 8.95 μmol Trolox g-1 lipid) 20.55 0.60 assays (918.56 27.55 lipid). study demonstrates potential source natural bioactive molecules compounds.

Язык: Английский

Unraveling the neutral and polar lipidome of Nordic brown macroalgae: A sustainable source of functional lipids DOI Creative Commons
Thu Huong Pham, Raymond Thomas, Clarissa Schwab

и другие.

Food Chemistry, Год журнала: 2024, Номер 459, С. 140415 - 140415

Опубликована: Июль 14, 2024

Brown macroalgae represent a sustainable and abundant source of lipids with acknowledged functional health benefits. Nonetheless, lipidome has been poorly unraveled due to complex structural chemical diversity. In this study, comprehensive lipidomic analysis was performed in four macroalgae: Saccharina latissima, Fucus vesiculosus, serratus the invasive Sargassum muticum, using HILIC-C30RP-HRMS. Neutral (tri-, di-glycerides) comprised 72–82% total (TL) highly unsaturation profile (27–49% depending on species). The polar glycolipids, phospholipids, betaine sphingolipids varied content among macroalgae. S. latissima displayed greatest level glycolipids (23% TL), by contrast dominance long-chain polyunsaturated (10–18% TL) other species, particularly muticum. Phospholipids were detected low abundance (<1.7% TL). This study elevated potential brown as an emerging reservoir bioactive nutritional relevance.

Язык: Английский

Процитировано

3

Algal Adaptation to Environmental Stresses: Lipidomics Research DOI Creative Commons
Ksenia Chadova

International Journal of Plant Biology, Год журнала: 2024, Номер 15(3), С. 719 - 732

Опубликована: Июль 22, 2024

Algal lipidomics is a new field of research that gaining increasing popularity. The use high-performance liquid chromatography–mass spectrometry (HPLC-MS) has made it possible to accurately determine the structure each lipid molecule in sample. Since algae are considered as promising source various compounds with pharmacological and biotechnological potential, including bioactive lipids polyunsaturated fatty acids, this group organisms particular interest. lipidome high plasticity, which due influence abiotic biotic environmental factors, observed changes composition are, rule, adaptive reactions. This review examines current algal lipidomics, discusses results studying such temperature, light intensity, nutrient concentration, epi- endophytic infections on lipidome, seasonal geographical plasticity lipidome; questions about adaptation mechanisms at level individual molecular species considered, gaps area noted.

Язык: Английский

Процитировано

3

Revealing the polar lipidome, pigment profiles, and antioxidant activity of the giant unicellular green alga, Acetabularia acetabulum DOI
Felisa Rey, Paulo Cartaxana, Sónia Cruz

и другие.

Journal of Phycology, Год журнала: 2023, Номер 59(5), С. 1025 - 1040

Опубликована: Июль 23, 2023

Marine algae are one of the most important sources high-value compounds such as polar lipids, omega-3 fatty acids, photosynthetic pigments, or secondary metabolites with interesting features for different niche markets. Acetabularia acetabulum is a macroscopic green single-celled alga, single nucleus hosted in rhizoid. This alga studied dasycladalean species and represents an model system cell biology studies. However, its lipidome pigment profile have been overlooked. Total lipid extracts were analyzed using hydrophilic interaction liquid chromatography-high resolution mass spectrometry (HILIC-HRMS), tandem (MS/MS), high-performance chromatography (HPLC). The antioxidant capacity was tested DPPH ABTS assays. Lipidomics identified 16 classes, corresponding to glycolipids, betaine phospholipids, sphingolipids, total 191 species, some them recognized by their bioactivities. abundant lipids glycolipids. Lipid classes less identified, diacylglyceryl-carboxyhydroxymethylcholine (DGCC) hexosylceramide (HexCer). A. comprised carotenoids (17.19%), namely cis-neoxanthin, violaxanthin, lutein β,β-carotene, chlorophylls b (82.81%). showed high activity promoting 50% inhibition (IC50 ) concentrations 57.91 ± 1.20 μg · mL-1 (438.18 8.95 μmol Trolox g-1 lipid) 20.55 0.60 assays (918.56 27.55 lipid). study demonstrates potential source natural bioactive molecules compounds.

Язык: Английский

Процитировано

0