Assessing eDNA capture method from aquatic environment to optimise recovery of human mt-eDNA DOI Creative Commons
Marie Antony Dass, Craig D. H. Sherman,

Roland A.H. van Oorschot

и другие.

Forensic Science International, Год журнала: 2024, Номер 361, С. 112085 - 112085

Опубликована: Июнь 4, 2024

Previous studies have shown that environmental DNA (eDNA) from human sources can be recovered natural bodies of water, and the generation profiles such samples may assist in forensic investigations. However, fundamental knowledge gaps exist around factors influencing probability detecting eDNA design optimal sampling protocols. One these is understanding particle sizes signals are most strongly associated with appropriate filter size needed for efficiently capturing particles. This study assessed amount mitochondrial different blood skin cells freshwater samples. Samples (300 mL) were taken experimental 10 L tanks spiked 50 µL or deposited by vigorously rubbing hands together two minutes freshwater. Subsamples collected passing 250 mL water sample through six pore (from 0.1 to 8 µm). process was repeated at four time intervals after spiking over 72 hours assess if changes as degrades. Using a human-specific quantitative polymerase chain reaction (qPCR) assay targeting HV1 gene region, total fractions determined. In case blood, 0 h, 0.45 µm captured greatest eDNA, 42 % detected. The pattern then changed 48 5 (67 %), 81 h. Notably, ten-fold dilution proved valuable strategy enhancing recovery all points, primarily due PCR inhibition observed hemoglobin. For cells, amounts consistent (capturing 37 %, 56 88 hours, respectively). There clear variation between cell types, some scenarios, there likely mix types present. These results suggest it would best use capture an maximize Depending on type contributing combination employed optimize provides groundwork optimizing efficient aquatic environments, paving way its broader application sciences.

Язык: Английский

Up in the air: Presence and collection of DNA from air and air conditioner units DOI Creative Commons
Mariya Goray, Duncan Taylor,

Emily Bibbo

и другие.

Electrophoresis, Год журнала: 2024, Номер 45(9-10), С. 933 - 947

Опубликована: Фев. 28, 2024

Abstract Biological material is routinely collected at crime scenes and from exhibits a key type of evidence during criminal investigations. Touch or trace DNA samples surfaces objects deemed to have been contacted are frequently collected. However, person interest may not leave any traces on surfaces, for example, if wearing gloves. A novel means sampling human air offers additional avenues collection. In the present study, we report results pilot study into prevalence persistence in air. The first aspect investigates conditioner units that circulate around room, by located four offices houses different time frames post‐cleaning. second ability collect rooms, with without people, periods types collection filters. Results this show can be unit air, representing more recent occupation while showing historic use room.

Язык: Английский

Процитировано

6
Where did it go? A study of DNA transfer in a social setting DOI Creative Commons

Amy Cahill,

Luke Volgin,

Roland A.H. van Oorschot

и другие.

Forensic Science International Genetics, Год журнала: 2024, Номер 73, С. 103101 - 103101

Опубликована: Авг. 2, 2024

Язык: Английский

Процитировано

4
The Transfer, Prevalence, Persistence, and Recovery of DNA from Body Areas in Forensic Science: A Review DOI Creative Commons

Cara Woollacott,

Mariya Goray,

Roland A.H. van Oorschot

и другие.

Forensic Sciences, Год журнала: 2025, Номер 5(1), С. 9 - 9

Опубликована: Фев. 14, 2025

Forensic and medical examiners are often required to sample the body of a victim (either living or deceased), suspect criminal offence, for foreign DNA. This can provide useful information when alleged activity involves presence various bodily fluids such as blood, semen, and/or saliva, well skin contact made between perpetrator victim. Optimal recovery techniques collection DNA evidence, following crime-relevant contact, be dependent on surface being sampled. Additional factors consider include areas typically contacted during activities likelihood non-self-DNA present in those prior contacts interest. Therefore, an understanding transfer, prevalence, persistence, aid interpretation results given activity-level questions increase value findings from this type evidence. review aims summarise research DNA-TPPR concerning human surfaces different types activities. examines prevalence background surfaces, reported transfer associated with forms how cofounding affect persistence

Язык: Английский

Процитировано

0
Macrobial airborne environmental DNA analysis: A review of progress, challenges, and recommendations for an emerging application DOI Creative Commons
Mark D. Johnson, Matthew A. Barnes

Molecular Ecology Resources, Год журнала: 2024, Номер 24(7)

Опубликована: Авг. 8, 2024

In the context of looming global biodiversity loss, effective species detection represents a critical concern for ecological research and management. Environmental DNA (eDNA) analysis, which refers to collection taxonomic identification genetic fragments that are shed from an organism into its surroundings, emerged approximately 15 years ago as sensitive tool detection. Today, one frontiers eDNA concerns analysis material in dust other airborne materials, termed analysis. As study matures, it is appropriate time review foundational emerging studies make up current literature, use reviewed literature summarize, synthesize, forecast major challenges opportunities this advancing front. Specifically, we "ecology eDNA" framework organize our findings across origin, state, transport, fate materials environment, summarize what so far known their interactions with surrounding abiotic biotic factors, including population community ecologies ecosystem processes. Within work identify key challenges, opportunities, future directions associated application development. Lastly, discuss development applications, partnerships, messaging promote growth field. Together, broad potential rate at accelerating field suggest sky's limit science.

Язык: Английский

Процитировано

2
Air DNA forensics: Novel air collection method investigations for human DNA identification DOI Open Access

Emily Bibbo,

Duncan Taylor,

Roland A.H. van Oorschot

и другие.

Journal of Forensic Sciences, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 10, 2024

Modern techniques can generate highly discriminatory DNA profiles from minuscule biological samples, providing valuable information in criminal investigations and court proceedings. However, trace touch due to their nature, often have lower success rates than other materials, such as blood. Further, forensically aware criminals utilize gloves meticulously clean the crime scene remove traces of themselves contacted surfaces. Air sampling offers a novel approach collection human that has potential bypass some these issues. This study reports on results research into prevalence persistence air. The ability collect air was investigated with use an AirPrep Cub Sampler ACD220 different spaces, without presence individuals for various durations sample collection. Results this demonstrate level occupation duration each influence quantity quality recovered whereas effects orientation distance participants device well sequence remain unclear require further investigation.

Язык: Английский

Процитировано

2
Transfer and persistence of intruder DNA within an office after reuse by owner DOI Creative Commons

Monique Zacher,

Roland A.H. van Oorschot,

Oliva Handt

и другие.

Forensic Science International Genetics, Год журнала: 2024, Номер 73, С. 103130 - 103130

Опубликована: Авг. 28, 2024

The heightened sensitivity of DNA typing techniques, paired with the extensive use trace in forensic investigations, has resulted an increased need to understand how and when is deposited on surfaces interest. This study focussed transfer, persistence, prevalence a single occupation office space by intruder, all contacts made during for two hours prior post were known. extent which could be recovered from contacted/not contacted was investigated. investigates impacts these movements duration occupancy, surface contact histories shedder status participants are Contacts documented targeted sampling. Categories set target sampling that included different types contact. Direct indirect transfer detected 55 % 6 samples, respectively. Contactless 0.5 samples. owner observed as sole/major/majority contributor 77 samples minor 10 intruder 14 16 %. An number relative contribution individual making contact, however, not direct detectable transfer. outcome this will aid better sample targeting strategies contribute pool data assisting development activity level assessments.

Язык: Английский

Процитировано

1
Assessing eDNA capture method from aquatic environment to optimise recovery of human mt-eDNA DOI Creative Commons
Marie Antony Dass, Craig D. H. Sherman,

Roland A.H. van Oorschot

и другие.

Forensic Science International, Год журнала: 2024, Номер 361, С. 112085 - 112085

Опубликована: Июнь 4, 2024

Previous studies have shown that environmental DNA (eDNA) from human sources can be recovered natural bodies of water, and the generation profiles such samples may assist in forensic investigations. However, fundamental knowledge gaps exist around factors influencing probability detecting eDNA design optimal sampling protocols. One these is understanding particle sizes signals are most strongly associated with appropriate filter size needed for efficiently capturing particles. This study assessed amount mitochondrial different blood skin cells freshwater samples. Samples (300 mL) were taken experimental 10 L tanks spiked 50 µL or deposited by vigorously rubbing hands together two minutes freshwater. Subsamples collected passing 250 mL water sample through six pore (from 0.1 to 8 µm). process was repeated at four time intervals after spiking over 72 hours assess if changes as degrades. Using a human-specific quantitative polymerase chain reaction (qPCR) assay targeting HV1 gene region, total fractions determined. In case blood, 0 h, 0.45 µm captured greatest eDNA, 42 % detected. The pattern then changed 48 5 (67 %), 81 h. Notably, ten-fold dilution proved valuable strategy enhancing recovery all points, primarily due PCR inhibition observed hemoglobin. For cells, amounts consistent (capturing 37 %, 56 88 hours, respectively). There clear variation between cell types, some scenarios, there likely mix types present. These results suggest it would best use capture an maximize Depending on type contributing combination employed optimize provides groundwork optimizing efficient aquatic environments, paving way its broader application sciences.

Язык: Английский

Процитировано

0