The use of non-lethal sampling for transcriptomics to assess the physiological status of wild fishes

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, Год журнала: 2021, Номер 256, С. 110629 - 110629

Опубликована: Май 28, 2021

Язык: Английский

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Wildlife ecological risk assessment in the 21st century: Promising technologies to assess toxicological effects

Integrated Environmental Assessment and Management, Год журнала: 2023, Номер 20(3), С. 725 - 748

Опубликована: Июль 7, 2023

Despite advances in toxicity testing and the development of new approach methodologies (NAMs) for hazard assessment, ecological risk assessment (ERA) framework terrestrial wildlife (i.e., air-breathing amphibians, reptiles, birds, mammals) has remained unchanged decades. While survival, growth, reproductive endpoints derived from whole-animal tests are central to nonstandard measures biological effects at multiple levels organization (e.g., molecular, cellular, tissue, organ, organism, population, community, ecosystem) have potential enhance relevance prospective retrospective ERAs. Other factors indirect contaminants on food supplies infectious disease processes) influenced by toxicants individual, community levels, need be factored into chemically based assessments "eco" component Regulatory logistical challenges often relegate such postregistration evaluations pesticides industrial chemicals contaminated site evaluations. NAMs being developed, date, their applications ERAs focused been limited. No single magic tool or model will address all uncertainties assessment. Modernizing likely entail combinations laboratory- field-derived data organization, knowledge collection solutions systematic review, adverse outcome pathway frameworks), inferential methods that facilitate integrations estimations species, populations, interspecific extrapolations, ecosystem services modeling, with less dependence simple ratios. Integr Environ Assess Manag 2024;20:725-748. © 2023 His Majesty King Right Canada The Authors. Integrated Environmental Assessment Management published Wiley Periodicals LLC behalf Society Toxicology & Chemistry (SETAC). Reproduced permission Minister Environment Climate Change Canada. This article contributed US Government employees work is public domain USA.

Язык: Английский

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Apical and mechanistic effects of 6PPD-quinone on different life-stages of the fathead minnow (Pimephales promelas)

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Год журнала: 2023, Номер 271, С. 109697 - 109697

Опубликована: Июль 12, 2023

Язык: Английский

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Toxicological effects assessment for wildlife in the 21st century: Review of current methods and recommendations for a path forward

Integrated Environmental Assessment and Management, Год журнала: 2023, Номер 20(3), С. 699 - 724

Опубликована: Июнь 1, 2023

Model species (e.g., granivorous gamebirds, waterfowl, passerines, domesticated rodents) have been used for decades in guideline laboratory tests to generate survival, growth, and reproductive data prospective ecological risk assessments (ERAs) birds mammals, while officially adopted assessment schemes amphibians reptiles do not exist. There are recognized shortcomings of current vivo methods as well uncertainty around the extent which with different life histories terrestrial amphibians, reptiles, bats) than these commonly models protected by existing ERA frameworks. Approaches other validating additional animal testing being developed, but incorporation such new approach methodologies (NAMs) into frameworks will require robust validations against responses. This takes time, ability extrapolate findings from nonanimal studies organism- population-level effects wildlife remains weak. Failure adequately anticipate predict hazards could economic potentially even legal consequences regulators product registrants. In order be able use fewer animals or replace them altogether long term, vertebrate whole organism needed provide NAM validation short term. Therefore, it is worth investing resources potential updates standard test guidelines addressing need clear guidance on conduct field studies. Herein, we review improving advancing assessment, tools foreseeable future. Integr Environ Assess Manag 2024;20:699-724. © 2023 His Majesty King Right Canada The Authors. Integrated Environmental Assessment Management published Wiley Periodicals LLC behalf Society Toxicology & Chemistry (SETAC). Reproduced permission Minister Environment Climate Change Canada. article has contributed U.S. Government employees their work public domain USA.

Язык: Английский

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Innovation in regulatory approaches for endocrine disrupting chemicals: The journey to risk assessment modernization in Canada

Environmental Research, Год журнала: 2021, Номер 204, С. 112225 - 112225

Опубликована: Окт. 18, 2021

Globally, regulatory authorities grapple with the challenge of assessing hazards and risks to human ecosystem health that may result from exposure chemicals disrupt normal functioning endocrine systems. Rapidly increasing number in commerce, coupled reliance on traditional, costly animal experiments for hazard characterization - often limited sensitivity many important mechanisms disruption -, presents ongoing challenges chemical regulation. The consequence is a which there sufficient data assess if toxicity hence few thorough characterization. To address this challenge, assessment disrupting (EDCs) benefiting revolution toxicology focuses New Approach Methodologies (NAMs) more rapidly identify, prioritize, potential using novel, efficient, mechanistically driven methodologies tools. Incorporated into Integrated Approaches Testing Assessment (IATA) guided by conceptual frameworks such as Adverse Outcome Pathways (AOPs), emerging approaches focus initially molecular interactions between test potentially vulnerable biological systems instead need data. These new testing methods can be complemented silico computational approaches, including those predict kinetics. Coupled data, these will inform risk-based decision-making approaches. Canada part global network collaborating building confidence use NAMs EDCs. Herein, we review current EDC regulation globally (mainly perspective health), provide how advances applied discuss promises faced adopting novel minimize due Canada, our world.

Язык: Английский

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New Approach Methodologies for the Endocrine Activity Toolbox: Environmental Assessment for Fish and Amphibians

Environmental Toxicology and Chemistry, Год журнала: 2023, Номер 42(4), С. 757 - 777

Опубликована: Фев. 15, 2023

Multiple in vivo test guidelines focusing on the estrogen, androgen, thyroid, and steroidogenesis pathways have been developed validated for mammals, amphibians, or fish. However, these tests are resource-intensive often use a large number of laboratory animals. Developing alternatives is consistent with replacement, reduction, refinement principles animal welfare considerations, which supported by increasing mandates to move toward an "animal-free" testing paradigm worldwide. New approach methodologies (NAMs) hold great promise identify molecular, cellular, tissue changes that can be used predict effects reliably more efficiently at individual level (and potentially populations) while reducing animals (eco)toxicological endocrine disruption. In collaborative effort, experts from government, academia, industry met 2020 discuss current challenges activity assessment fish amphibians. Continuing this cross-sector initiative, our review focuses state science regarding NAMs chemical-induced effects. The present study highlights using safety what work needed reduce their uncertainties increase acceptance regulatory processes. We reviewed available including silico, vitro, eleutheroembryo models. integrated as part weight-of-evidence hazard risk adverse outcome pathway framework. development utilization not only allows but also provide robust fit-for-purpose methods chemicals acting via mechanisms. Environ Toxicol Chem 2023;42:757-777. © 2023 Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC behalf SETAC.

Язык: Английский

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Combining species sensitivity distribution (SSD) model and thermodynamic index (exergy) for system-level ecological risk assessment of contaminates in aquatic ecosystems

Environment International, Год журнала: 2019, Номер 133, С. 105275 - 105275

Опубликована: Окт. 29, 2019

After reviewing the species- and community-level ecological risk assessments (ERAs) of chemicals in aquatic environment, present study attempted to propose a third stage ERA, i.e., ecosystem-level ERA. Based on species sensitivity distribution model (SSD) thermodynamic theory, exergy biomass indicators communities from various trophic levels (TLs) were introduced improve connotation SSDs. The classified into three TLs based algae (TL1), invertebrates (TL2), vertebrates (TL3), weight each TL was determined relative β value, which indicated holistic contribution or community ecosystem. Then, system-level ERA protocol successfully established, community- risks 10 typical toxic micro-organic pollutants western area Lake Chaohu its inflowing rivers evaluated. System-level curves (ExSSD) mainly affected by SSD at TL2 for most study. uncertain boundary ExSSD mostly related with wider boundary, but had little relation TL. results ERAs revealed that dibutyl phthalate highest eco-risk, whereas γ-hexachlorocyclohexane presented lowest eco-risk. Results not fully consistent those owing lack sufficient dataset, type, ecosystem structure, as successful application signifies start system-level, it also provides scientific basis protection, future water safety management. However, there some limitations, including data dependence, neglect interactions, environmental parameters such natural organic matter. We employ toxicogenomics enrich toxicity database, simulate interaction using dynamic model, introduce chemical fate

Язык: Английский

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Literature review and evaluation of biomarkers, matrices and analytical methods for chemicals selected in the research program Human Biomonitoring for the European Union (HBM4EU)

Environment International, Год журнала: 2022, Номер 169, С. 107458 - 107458

Опубликована: Авг. 6, 2022

Humans are potentially exposed to a large amount of chemicals present in the environment and workplace. In European Human Biomonitoring initiative (Human for Union = HBM4EU), acrylamide, mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1), diisocyanates (4,4'-methylenediphenyl diisocyanate, 2,4- 2,6-toluene diisocyanate), pyrethroids were included among prioritized concern human health. For literature review, analytical methods used worldwide biomonitoring studies these compounds collected presented comprehensive tables, including following parameter: determined biomarker, matrix, sample amount, work-up procedure, available laboratory quality assurance assessment information, techniques, limit detection. Based on data most suitable recommended. According paradigm biomonitoring, information about two different biomarkers exposure was evaluated: a) internal dose parent metabolites urine blood; b) biologically effective measured as blood protein adducts. Urine preferred matrix (biomarkers dose). Markers biological hemoglobin adducts serum-albumin-adducts aflatoxin B1 diisocyanates. The analyses quantitation or mostly performed with LC-MS/MS GC-MS presence isotope-labeled standards. This review also addresses critical aspects application, use selection biomarkers. future studies, more approach is discussed broaden compounds.

Язык: Английский

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An Early–Life Stage Alternative Testing Strategy for Assessing the Impacts of Environmental Chemicals in Birds

Environmental Toxicology and Chemistry, Год журнала: 2019, Номер 39(1), С. 141 - 154

Опубликована: Авг. 26, 2019

Abstract Early–life stage (ELS) toxicity tests are recognized as an advancement over current testing methodologies in terms of cost, animal use, and biological relevance. However, standardized ELS not presently available for some vertebrate taxa, including birds. The present study describes a Japanese quail ( Coturnix japonica ) test that is promising candidate standardization applies it to 8 environmental chemicals (ethinylestradiol, benzo[ ]pyrene, chlorpyrifos, fluoxetine, lead(II)nitrate, trenbolone, seleno‐L‐methionine, hexabromocyclododecane). Individual were injected into the air cell unincubated eggs at 3 concentrations, all predicted cause ≤20% mortality. Survival embryonic day 16 was consistently high (>90%) among vehicle‐injected controls. All chemicals, except ethinylestradiol, detected liver tissue, most concentrations suggestive clearance. Adverse effects observed 5 chemicals; chlorpyrifos (41.1 µg/g) significantly increased developmental abnormalities decreased embryo gallbladder mass. Ethinylestradiol (54.2 hexabromocyclododecane (0.02 mass tarsus length, respectively. Benzo[ ]pyrene (0.83 fluoxetine hydrochloride (32.7 exceeded 20% mortality cutoff. No following or trenbolone exposure up 10.7, 0.07, 4.4 µg/g, Overall, our approach time‐ cost‐effective, caused minimal controls, effectively delivered diverse embryo, permitted identification apical outcomes, which provide support toward standardization. Environ Toxicol Chem 2019;39:141–154. © 2019 SETAC

Язык: Английский

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Drivers of and Obstacles to the Adoption of Toxicogenomics for Chemical Risk Assessment: Insights from Social Science Perspectives

Environmental Health Perspectives, Год журнала: 2020, Номер 128(10)

Опубликована: Окт. 1, 2020

Background: Some 20 y ago, scientific and regulatory communities identified the potential of omics sciences (genomics, transcriptomics, proteomics, metabolomics) to improve chemical risk assessment through development toxicogenomics. Recognizing that regulators adopt new methods cautiously given accountability diverse stakeholders, scope pace adoption toxicogenomics tools data have nonetheless not met ambitious, early expectations proponents. Objective: Our objective was, therefore, inventory, investigate, derive insights into drivers obstacles in assessment. By invoking established social science frameworks conceptualizing innovation adoption, we also aimed develop recommendations for proponents other approach methodologies (NAMs). Methods: We report findings from an analysis 56 publications 1998 2017 address From this purposeful sample discourse, major categories sets. then mapped these onto generate actionable Discussion: identify most salient obstacles. 2017, was understood be helped by such as those labeled Superior understanding, New applications, Reduced cost & increased efficiency but hindered Insufficient validation, Complexity interpretation, Lack standardization. Leveraging frameworks, find arguments draw on drivers, which emphasize superior novel functionality rationales, overlook adopters' key concerns: simplicity use compatibility with existing practices. two perspectives—innovation-centric adopter-centric—on explain how overreliance former may undermining efforts promote https://doi.org/10.1289/EHP6500

Язык: Английский

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