Asian Journal of Pharmaceutical Sciences,
Год журнала:
2024,
Номер
unknown, С. 101006 - 101006
Опубликована: Дек. 1, 2024
Herpes
simplex
virus
thymidine
kinase
(HSVtk)
gene
therapy
is
a
promising
strategy
for
glioblastoma
therapy.
However,
delivery
of
plasmid
DNA
(pDNA)
encoding
HSVtk
into
the
brain
by
systemic
administration
challenge
since
pDNA
can
hardly
penetrate
blood-brain
barrier.
In
this
study,
an
exosome-membrane
(EM)
and
polymer-based
hybrid
complex
was
developed
brain.
Histidine/arginine-linked
polyamidoamine
(PHR)
used
as
carrier.
PHR
binds
to
electrostatic
interaction.
The
pDNA/PHR
mixed
with
EM
subjected
extrusion
produce
pDNA/PHR-EM
complex.
For
targeting,
T7
peptide
attached
Both
T7-decorated
(pDNA/PHR-EM-T7)
had
surface
charge
-5
mV
size
280
nm.
Transfection
assays
indicated
that
pDNA/PHR-EM-T7
enhanced
transfection
C6
cells
compared
pDNA/PHR-EM.
Intravenous
pHSVtk/PHR-EM-T7
showed
pHSVtk/PHR-EM
delivered
pHSVtk
more
efficiently
than
pHSVtk/lipofectamine
pHSVtk/PHR
in
vivo.
higher
efficiency
pHSVtk/PHR-EM.
As
result,
expression
apoptosis
levels
tumors
group
were
those
other
control
groups.
Therefore,
useful
carrier
glioblastoma.
RNA
interference
(RNAi)
represents
a
promising
gene-specific
therapy
against
tumors.
However,
its
clinical
translation
is
impeded
by
poor
performance
of
lysosomal
escape
and
tumor
targeting.
This
challenge
especially
prominent
in
glioblastoma
(GBM)
therapy,
necessitating
the
penetration
blood-brain
barrier
(BBB).
Leveraging
intrinsic
tumor-targeting
BBB
traversing
capability
human
H-ferritin,
we
designed
series
ferritin
variants
with
positively
charged
cavity
truncated
carboxyl
terminus,
termed
tHFn(+).
These
nanocarriers
respond
to
weak
acid
disassemble
endosomal
compartments,
exposing
internal
positive
charges
facilitate
loaded
small
interfering
(siRNA).
Functioning
as
universal
siRNA
nanocarriers,
tHFn(+)
significantly
enhanced
uptake
different
siRNAs
suppressed
gene
expressions
associated
GBM
progression.
Furthermore,
traversed
targeted
glioma
vivo
binding
receptors
(e.g.,
transferrin
receptor
1).
tHFn(+)-delivered
exhibited
exceptional
therapeutic
effects
vivo,
advancing
RNAi
therapeutics
beyond
for
treatment
various
diseases.
Bioconjugate Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Brain
tumors,
particularly
glioblastomas,
represent
the
most
complicated
cancers
to
treat
and
manage
due
their
highly
invasive
nature
protective
barriers
of
brain,
including
blood-brain
barrier
(BBB).
The
efficacy
currently
available
treatments,
viz.,
radiotherapy,
chemotherapy,
immunotherapy,
are
frequently
limited
by
major
side
effects,
drug
resistance,
restricted
penetration
into
brain.
Lipid
nanoparticles
(LNPs)
have
emerged
as
a
promising
targeted
delivery
system
for
brain
tumors.
nanocarriers
gained
tremendous
attention
tumor
therapeutics
multiple
encapsulation
abilities,
controlled
release,
better
biocompatibility,
ability
cross
BBB.
Herein,
detailed
analysis
design,
mechanisms,
therapeutic
benefits
LNPs
in
treatment
is
discussed.
Moreover,
we
also
discuss
safety
issues
clinical
developments
current
future
challenges.
Further,
focused
on
transformation
therapy
eliminating
effects
engineering
overcome
related
biological
barriers,
which
provide
personalized,
affordable,
low-risk
options.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(14), С. 7715 - 7715
Опубликована: Июль 14, 2024
Exosomes
are
small
vesicles
containing
proteins,
nucleic
acids,
and
biological
lipids,
which
responsible
for
intercellular
communication.
Studies
have
shown
that
exosomes
can
be
utilized
as
effective
drug
delivery
vehicles
to
accurately
deliver
therapeutic
substances
target
tissues,
enhancing
effects
reducing
side
effects.
Mesenchymal
stem
cells
(MSCs)
a
class
of
widely
used
tissue
engineering,
regenerative
medicine,
immunotherapy.
derived
from
MSCs
special
immunomodulatory
functions,
low
immunogenicity,
the
ability
penetrate
tumor
high
yield,
expected
engineered
into
efficient
systems.
Despite
promising
promise
MSC-derived
exosomes,
exploring
their
optimal
preparation
methods,
drug-loading
modalities,
potential
remains
challenging.
Therefore,
this
article
reviews
related
characteristics,
application,
risks
systems
in
order
find
breakthroughs.
European Journal of Pharmaceutics and Biopharmaceutics,
Год журнала:
2024,
Номер
203, С. 114460 - 114460
Опубликована: Авг. 31, 2024
Glioblastoma
(GBM)
stands
for
the
most
common
and
aggressive
type
of
brain
tumour
in
adults.
It
is
highly
invasive,
which
explains
its
short
rate
survival.
Little
known
about
risk
factors,
current
therapy
still
ineffective.
Hence,
efforts
are
underway
to
develop
novel
effective
treatment
approaches
against
this
cancer.
Exosomes
being
explored
as
a
promising
strategy
conveying
delivering
therapeutic
cargo
GBM
cells.
They
can
fuse
with
cell
membrane
and,
consequently,
serve
delivery
systems
context.
Due
their
nanoscale
size,
exosomes
cross
blood-brain
barrier
(BBB),
constitutes
significant
hurdle
chemotherapeutic
drugs
used
GBM.
subsequently
inhibit
oncogenes,
activate
suppressor
genes,
induce
immune
responses,
control
growth.
However,
despite
representing
tool
GBM,
further
research
clinical
studies
regarding
exosome
biology,
engineering,
applications
need
be
completed.
Here,
we
sought
review
application
through
an
in-depth
analysis
scientific
on
entire
process,
from
isolation
purification
design
transformation
into
anti-oncogenic
drug
systems.
Surface
modification
enhance
BBB
penetration
GBM-cell
targeting
also
topic
discussion.
Abstract
Over
the
past
few
decades,
immunotherapy
has
emerged
as
a
powerful
strategy
to
overcome
limitations
of
conventional
cancer
treatments.
The
use
extracellular
vesicles,
particularly
exosomes,
which
carry
cargoes
capable
modulating
immune
response,
been
extensively
explored
potential
therapeutic
approach
in
immunotherapy.
Exosomes
can
deliver
their
cargo
target
cells,
thereby
influencing
phenotype
and
immunomodulatory
functions.
They
exhibit
either
immunosuppressive
or
immune‐activating
characteristics,
depending
on
internal
contents.
These
exosomes
originate
from
diverse
cell
sources,
contents
vary,
suggesting
that
there
may
be
delicate
balance
between
suppression
stimulation
when
utilizing
them
for
Therefore,
thorough
understanding
molecular
mechanisms
underlying
role
progression
is
essential.
This
review
focuses
driving
exosome
function
impact
tumor
microenvironment
(TME),
highlighting
intricate
activation
must
navigated
exosome‐based
therapies.
Additionally,
it
underscores
challenges
ongoing
efforts
optimize
immunotherapies,
making
significant
contribution
advancement
research.
ABSTRACT
Stroke
remains
the
leading
cause
of
neurological
mortality
and
disability
worldwide,
with
post‐stroke
inflammation
significantly
hindering
neural
repair.
Despite
its
critical
impact,
mechanism‐based
therapeutic
strategies
are
scarce.
In
this
study,
we
uncovered
a
critically
important
yet
previously
unexamined
cell
population,
p21
+
CD86
microglia,
which
accumulated
in
ischemic
region.
Unexpectedly,
discovered
that
interacted
C/EBPβ,
driving
C/EBPβ‐dependent
transcription
upregulating
key
pro‐inflammatory
factors
such
as
Il6
,
Il1β
Cxcl2
Cxcl10
.
To
specifically
target
eliminate
these
pathogenic
engineered
exosomes
peptide
selectively
binds
microglia
loaded
them
senolytic
Quercetin.
Furthermore,
developed
an
optimized,
stable
Que@micro‐Exo
formulation.
Systemic
administration
robustly
reduced
suppressed
their
phenotype.
Notably,
functional
analyses
revealed
treatment
mitigated
blood‐brain
barrier
disruption,
promoted
beneficial
microglial
polarization,
decreased
neutrophil
infiltration,
enhanced
recovery
following
cerebral
ischemia,
all
favorable
safety
profile.
Our
preclinical
findings
lay
foundation
for
targeting
novel
strategy,
highlighting
potential
exosome‐based
anti‐inflammatory
therapy
stroke
other
central
nervous
system
disorders.
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
13
Опубликована: Март 17, 2025
Bone
defect,
a
common
orthopedic
condition,
is
characterized
by
lengthy
and
impactful
treatment
period,
posing
considerable
challenge
in
clinical
settings.
Medical
technology
has
advanced
notably,
effectively
treated
an
increasing
number
of
patients
with
bone
defects.
Consequently,
there
been
explosion
research
articles
on
regeneration,
including
substantial
the
application
exosomes.
Exosomes,
especially
those
derived
from
stem
cells,
have
confirmed
to
be
effective
regeneration
garnered
widespread
attention
last
decade.
Therefore,
this
study
conducted
bibliometric
analysis
publications
related
exosomes
for
regeneration.
The
objectives
are
explore
development
history
hotspots
field
over
past
10
years,
predict
future
trends,
provide
guidance
subsequent
research.
Web
Science
Core
Collection
(WoSCC)
database
was
searched
published
1
January
2014,
31
December
2023.
collected
literature
analyzed
using
software
such
as
Microsoft
Excel,
CiteSpace
6.3R1,
VOSviewer
1.6.20,
online
platform
(https://bibliometric.com).
A
total
3,004
2,729
institutions
68
countries
were
included
study.
increased
annually
China
most
prolific
country
field,
1,468
papers;
Shanghai
Jiao
Tong
University
(China)
institution
highest
(117
publications).
In
terms
authors,
Xin
Wang,
Yi
Zhang,
Yang
Wang
three
who
papers,
14
papers
each.
Co-citation
revealed
that
article
Valadi
H
2007
co-citations
(270
times
quotation).
Additionally,
focused
function
mechanism
action.
Furthermore,
importance
osteoblast
differentiation
angiogenesis
also
significant
scholars
field.
This
reviewed
achievements
utilizing
tools.
It
visualized
countries,
institutions,
journals
made
contributions
current
hotspots,
finally
explored
trends.
