Insights of species-specific polar lipidome signatures of seaweeds fostering their valorization in the blue bioeconomy

Algal Research, Год журнала: 2021, Номер 55, С. 102242 - 102242

Опубликована: Март 7, 2021

The valorization of seaweeds has generated unprecedented interest in recent years due to their unique chemical characteristics. taxonomic differentiation between Chlorophyta, Rhodophyta, and Ochrophyta is often supported by contrasting Polar lipids are the most abundant class several species labelled as valuable phytochemicals. Nevertheless, little still known about how polar lipidome varies within among different phyla. In this study, six farmed under controlled conditions a land-based integrated multitrophic aquaculture (IMTA), belonging Chlorophyta (Ulva rigida Codium tomentosum), Rhodophyta (Gracilaria gracilis, Palmaria palmata Porphyra dioica), (Fucus vesiculosus) was characterized using state art high-resolution LC-MS analysis. were harvested during same season time window control biotic abiotic shaping lipidome. total, 477 (m/z values) identified distributed over surveyed. Only small group 35 shared all seaweed analyzed study. distribution lipid classes showed trend towards grouping phylum although specie specific signatures can be assigned. Matching data gathered present study with those previously acquired species-specific clustering, which confirm uniqueness these natural biochemical fingerprints. Recognition necessary because assigned important bioactive properties. This knowledge will allow designing new strategies for tuning farming so that yield target compounds improved, living resources fully contribute sustainable growth blue bioeconomy.

Язык: Английский

---

The response mechanisms of kelp Macrocystis pyrifera holobiont to elevated temperature and CO2 concentration

Journal of Applied Phycology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Язык: Английский

---

Identification of Cocconeis neothumensis var. marina using a polyphasic approach including ultrastructure and gene annotation

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0317360 - e0317360

Опубликована: Фев. 13, 2025

Several microalgae, including marine diatoms, significantly contribute to the global primary production and play a vital role in food webs of benthic planktonic ecosystems. Diatoms genus Cocconeis frequently inhabit substrates, leaves seagrasses. They are seasonally dominant leaf epiphytic layer Mediterranean seagrass Posidonia oceanica L. Delile, have been proposed as model organisms for chemical ecology studies. However, genome spp. has not sequenced. Consequently, their low-level molecular identification is currently impossible, besides few examples. To address this gap, polyphasic C. neothumensis employed, combining ultra-morphological data with DNA barcoding markers. A strain diatoms was isolated from P. leaves. It cultured laboratory examined under Scanning Electron Microscopy (SEM). The 18S ribosomal RNA gene (18S rRNA, nrDNA) ribulose 1,5-biphosphate carboxylase (rbc L, cpDNA) were analysed characterisation. Since ultra-morphology unambiguously identified Krammer, 1991, sequences herein reported will facilitate its rapid accurate identification. In addition, our comparative analyses evaluation these markers closely related diatoms.

Язык: Английский

---

Describing the complex chemistry of benthic seawater: from exometabolite sampling strategies to MS-based metabolomics

Natural Product Reports, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

This review highlights advances in characterizing exometabolites (EMs) from benthic organisms, starting with situ sampling methods, then discussing how marine MS-based (exo)metabolomics benefits various fields while addressing ongoing challenges.

Язык: Английский

---

Towards a mechanistic understanding of microalgae–bacteria interactions: integration of metabolomic analysis and computational models

FEMS Microbiology Reviews, Год журнала: 2022, Номер 46(5)

Опубликована: Май 6, 2022

Abstract Interactions amongst marine microalgae and heterotrophic bacteria drive processes underlying major biogeochemical cycles are important for many artificial systems. These dynamic complex interactions span the range from cooperative to competitive, it is diverse intricate networks of metabolites chemical mediators that predicted principally dictate nature relationship at any point in time. Recent advances technologies identify, analyze, quantify have allowed a comprehensive view molecules available exchange and/or reflective organismal interactions, setting stage development mechanistic understanding these Here, we (i) review current knowledge landscape microalgal–bacterial by focusing on metabolomic studies selected, simplified model systems; (ii) describe state field metabolomics, with specific focus techniques approaches developed microalga–bacterial interaction studies; (iii) outline main mathematical models interacting systems, which collectively power enhance interpretation experimental data generate novel testable hypotheses. We share viewpoint integrated series -omics include theoretical formulations necessary develop predictive biological entities.

Язык: Английский

---

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Scenedesmus subspicatus Unraveled by Quantitative Proteomics

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Май 7, 2020

Microalgae can respond to natural cues from crustacean grazers, such as Daphnia, by forming colonies and aggregations called flocs. Combining microalgal biology, physiological ecology, quantitative proteomics, we identified how infochemicals Daphnia trigger cellular level changes in the microalga Scenedesmus subspicatus, underpinning colony formation flocculation. We discovered that flocculation occurs at an energy-demanding 'alarm' phase, with important role proposed cysteine synthesis. Flocculation appeared be initially stimulated production of extracellular matrix where polysaccharides fatty acids were present, later sustained 'acclimation' stage through mitogen-activated protein kinase (MAPK) signalling cascades. Colony required investment into acid metabolism, likely linked separation membranes during cell division. Higher energy demands alarm which subsequently decreased acclimation stage, thus suggesting a trade-off between From ecological evolutionary perspective, our findings represent improved understanding effect on microalgae-grazers interactions, they therefore potentially impact structure aquatic communities. Moreover, mechanisms revealed are interest algal biotechnology, for exploitation low-cost, sustainable biomass harvesting.

Язык: Английский

---

Comparative volatilomics of coral endosymbionts from one- and comprehensive two-dimensional gas chromatography approaches

Marine Biology, Год журнала: 2021, Номер 168(5)

Опубликована: Апрель 28, 2021

Язык: Английский

---

A Metabolomics Exploration of the Sexual Phase in the Marine Diatom Pseudo-nitzschia multistriata

Marine Drugs, Год журнала: 2020, Номер 18(6), С. 313 - 313

Опубликована: Июнь 14, 2020

Pseudo-nitzschia multistriata is a planktonic marine diatom with diplontic life cycle comprising short sexual phase, during which gametes are produced following the encounter of two diploid cells opposite mating type (MT). Gene expression studies have highlighted presence substantial changes occurring at onset reproduction. Herein, we hypothesized that amount and nature cellular metabolites varies along process. To capture metabolome different harvesting times in an unbiased manner, undertook untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three extraction steps, method revealed pronounced differences metabolic profiles between control vegetative phase (MT+ MT−) mixed strains MTs (cross) undergoing Of 2408 high-quality features obtained, 70 known could be identified in-house libraries online databases; additional 46 classified by molecular networking tandem spectra. The reduction phytol detected cross can linked to general downregulation photosynthesis reproduction observed elsewhere. Moreover, role highly regulated compounds such as 7-dehydrodesmosterol, whose abundance were highest experiment, oleamide, ectoine, or trigonelline discussed.

Язык: Английский

---

Responses of the Macroalga Ulva prolifera Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches

Marine Drugs, Год журнала: 2022, Номер 20(12), С. 743 - 743

Опубликована: Ноя. 26, 2022

Ocean acidification (OA) is a dramatic perturbation of seawater environments due to increasing anthropogenic emissions CO2. Several studies indicated that OA frequently induces marine biota stress and reduction biodiversity. Here, we adopted the macroalga Ulva prolifera as model applied complementary multi-omics approach investigate metabolic profiles under normal acidified conditions. Our results show U. grows at higher rates in environments. Consistently, observed lower sucrose phosphocreatine concentrations response demand energy for growth availability essential amino acids, likely related increased protein biosynthesis. In addition, pathways leading signaling deterrent compounds appeared perturbed. Finally, remarkable shift was here first time fatty acid composition triglycerides, with decrease relative abundance PUFAs towards an appreciable increase palmitic acid, thus suggesting remodeling lipid Overall, our revealed modulation several biosynthetic conditions which, besides possible effects on ecosystem, changes alga should be taken into account considering its potential nutraceutical applications.

Язык: Английский

---

Preparation of a Cu₂O/g-C₃N₄ heterojunction with enhanced photocatalytic antibacterial activity under visible light

New Journal of Chemistry, Год журнала: 2023, Номер 47(29), С. 13797 - 13809

Опубликована: Янв. 1, 2023

In this study, a Cu 2 O/g-C 3 N 4 nanocomposite was prepared through in situ growth of O nanoparticles on g-C nanosheets.

Язык: Английский

---