In Situ Capture and Real-Time Enrichment of Marine Chemical Diversity

ACS Central Science, Год журнала: 2023, Номер 9(11), С. 2084 - 2095

Опубликована: Ноя. 8, 2023

Analyzing the chemical composition of seawater to understand its influence on ecosystem functions is a long-lasting challenge due inherent complexity and dynamic nature marine environments. Describing intricate chemistry requires optimal in situ sampling. Here presented novel underwater hand-held solid-phase extraction device, I-SMEL (In Situ Marine moleculE Logger), which aims concentrate diluted molecules from large volumes delimited zone targeting keystone benthic species. holobionts, such as sponges, can impact their surroundings possibly through production release specialized metabolites, hence termed exometabolites (EMs). was deployed sponge-dominated Mediterranean at 15 m depth. Untargeted MS-based metabolomics performed enriched EM extracts showed (1) diversity metabolites (2) reproducible recovery enrichment sponge EMs aerothionin, demethylfurospongin-4, longamide B methyl ester. These constitute identity each targeted species: Aplysina cavernicola, Spongia officinalis, Agelas oroides, respectively. concentrated 10 L water min sampling time. The present proof concept with opens new research perspectives ecology sets stage for further sustainable efforts natural product chemistry.

Язык: Английский

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Ecophysiological behaviour of different diatoms in response to copepod signals

Phycologia, Год журнала: 2024, Номер 63(1), С. 120 - 127

Опубликована: Янв. 2, 2024

Phytoplanktonic communities have been moulded from a higher level in the food web by zooplankton predation. Diatoms, which are major primary producers present day oceans, evolved many strategies to thrive presence of copepods, their predators. Chemical signals released aquatic environment microarthropods and elicit morphological, behavioural and/or physiological modifications diatom cells allow them cope with Other than most studied effects copepod on release toxins diatoms, role chemical cues growth biology non-toxic diatoms has little investigated, although it may broaden our knowledge co-evolutive mechanisms. To examine response copepod-derived signals, Phaeodactylum tricornutum, Chaetoceros muelleri Cylindrotheca fusiformis (Bacillariophyceae) were grown cues. Physiological occurred differed sign magnitude between species: i) P. tricornutum cell density was higher, dry weight lower, suggesting energy allocation into division at expense lipid protein quotas; ii) C. showed very homeostatic behaviour; iii) lower content showing lipids, likely act as deterrent feeders. Copepod here suggested ruled species composition ocean affecting species-specific performance macromolecules.

Язык: Английский

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Volatile‐mediated interactions with surface‐associated microbes: A parallelism between phyllosphere of plants and eco‐chemosphere of seaweeds

Journal of Ecology, Год журнала: 2021, Номер 109(8), С. 2823 - 2831

Опубликована: Май 17, 2021

Abstract Both in terrestrial and aquatic realms, organisms communicate interact with each other via volatile non‐volatile infochemicals. Terrestrial plants seaweeds, known as prolific producers of volatiles, harbour a plethora microbes on their surfaces like phyllosphere plant leaves eco‐chemosphere respectively, where complex ecological interactions are regulated through Although leaf volatiles have been well‐studied for functions mediating microbial interactions, seaweed mostly investigated roles climate regulation regard to change research. However, appear be related both terms chemistry ecology. Synthesis . Evidence supports that can important surface, just volatiles. Based the existing vast literature mediated by at very few works eco‐chemosphere, we advocate detailed investigation volatile‐mediated regulating colonisation processes surfaces. great importance, this new field research has remained largely unexplored. Thus, also set directions future programs investigating seaweed–microbe interface.

Язык: Английский

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Unraveling cellular and molecular mechanisms of acid stress tolerance and resistance in marine species: New frontiers in the study of adaptation to ocean acidification

Marine Pollution Bulletin, Год журнала: 2022, Номер 185, С. 114365 - 114365

Опубликована: Ноя. 23, 2022

Язык: Английский

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Bromination of Quorum Sensing Molecules: Vanadium Bromoperoxidase and Cerium Dioxide Nanocrystals via Free Active Bromine Transform Bacterial Communication

Environmental Science & Technology, Год журнала: 2023, Номер 57(47), С. 18491 - 18498

Опубликована: Май 24, 2023

The halogenation of quorum sensing molecules (QSMs) is known to be catalyzed by enzymes such as haloperoxidase (HPO) well cerium dioxide nanocrystals (NC), which mimic enzymes. Those and mimics can influence biological processes biofilm formation, where bacteria use QSMs for the "chemical" communication between each other coordination surface colonization. However, not much about degradation behavior a broad spectrum QSMs, especially HPO its mimics. Therefore, in this study, three with different molecule moieties was elucidated. For purpose, batch experiments were carried out HPOs, NCs free active bromine (FAB). N-β-ketocaproyl-homoserine lactone (3-Oxo-C6-AHL), N-cis-tetradec-9Z-enoyl-homoserine (C14:1-AHL) 2-heptyl-4-quinolone (HHQ) fast moiety-specific transformations observed. vanadium bromoperoxidase formation same brominated transformation products (TPs). Since TPs are formed FAB it very likely that playing major role catalytical reaction mechanism leading QSMs. In study total 17 could identified levels confidence catalytic two QS groups (unsaturated AHLs alkyl quinolones) expanded.

Язык: Английский

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