Phytochemical Analysis,
Год журнала:
2023,
Номер
35(2), С. 369 - 379
Опубликована: Окт. 19, 2023
Abstract
Introduction
Fungal
species
are
an
attractive
resource
for
physiologically
functional
food
and
drug
precursor.
Fomes
officinalis
Ames,
a
medicinal
fungus,
is
traditionally
used
as
folk
medicine
in
traditional
Chinese
prescription
the
therapy
of
cough
asthma.
The
water‐soluble
substances
herbal
medicines
likely
to
play
important
physiological
function.
However,
information
on
probing
identifying
chemical
components
aqueous
extract
Ames
(AFO)
remains
unknown.
Objective
This
study
was
conducted
screen
characterise
AFO.
Material
Methods
An
effective
sensitive
ultrahigh‐performance
liquid
chromatography
tandem
quadrupole‐Orbitrap
high‐resolution
mass
spectrometry
(UHPLC‐Q‐Orbitrap‐HRMS)
method
with
Full
MS/PIL/dd‐MS
2
acquisition
approach
applied
profiling
HSS
T3
column
component
separation,
strategy
simultaneous
targeted
untargeted
multicomponent
characterisation
implemented.
Multiple
identification
approaches
were
used,
including
accurate
molecular
elemental
composition
matching,
literature
database
searching,
fragmentation
rules
elucidation.
Results
A
total
115
components,
20
amino
acids
derivatives,
six
nucleobases,
nine
nucleosides,
75
dipeptides,
two
tripeptides,
three
other
tentatively
identified.
Among
them,
exploring
screened
nucleobases
nucleosides
modified
nucleosides.
To
our
best
knowledge,
this
first
time
report
has
been
done
presence
compounds
Conclusion
Profiling
AFO
enriched
its
material
basis,
which
would
lay
foundation
improving
potential
nutritional
values
effecting
comprehensive
quality
control
Ames.
Journal of Fungi,
Год журнала:
2025,
Номер
11(3), С. 205 - 205
Опубликована: Март 6, 2025
Helvella
leucopus,
an
endangered
wild
edible
fungus,
is
renowned
for
its
distinct
health
benefits
and
nutritional
profile,
with
notable
differences
in
the
bioactive
properties
between
cap
stipe.
To
investigate
molecular
basis
of
these
tissue-specific
variations,
we
conducted
integrative
transcriptomic
metabolomic
analyses.
Metabolomic
profiling
showed
that
particularly
rich
compounds,
including
sterols
alkaloids,
while
stipe
abundant
essential
nutrients,
such
as
glycerophospholipids
amino
acids.
Transcriptomic
analysis
revealed
a
higher
expression
genes
involved
sterol
biosynthesis
(ERG1,
ERG3,
ERG6)
energy
metabolism
(PGK1,
ENO1,
PYK1)
cap,
suggesting
more
active
metabolic
profile
this
tissue.
Pathway
enrichment
highlighted
pathways,
riboflavin
metabolism,
pantothenate
CoA
biosynthesis,
terpenoid
backbone
key
contributors
to
unique
functional
A
detailed
biosynthetic
pathway
network
further
illustrated
how
pathways
contribute
production
crucial
sterols,
linoleic
acid
derivatives,
glycerophospholipids,
acids,
each
These
findings
provide
significant
insights
into
mechanisms
behind
health-promoting
richness
stipe,
offering
theoretical
foundation
utilizing
H.
leucopus
food
development
broadening
our
understanding
distribution
fungi.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 9, 2025
Enzymatic
synthesis
of
flavor
esters
is
widely
used
in
the
food
and
industries,
but
challenges
remain
improving
catalytic
efficiency
stability
biocatalysts.
This
study
evaluates
performance
a
novel
biocatalyst,
CALB@DMSN-C8,
formed
by
immobilizing
Candida
antarctica
lipase
B
(CALB)
on
hydrophobic
dendritic
mesoporous
silica
nanospheres
(DMSN-C8),
for
synthesizing
esters.
The
CALB@DMSN-C8
catalyst
achieves
caproic
acid
conversion
rate
98.5
±
0.5%
just
30
min
demonstrates
outstanding
thermal
stability,
retaining
high
over
20
reuse
cycles.
To
our
knowledge,
this
represents
most
efficient
esters,
including
ethyl
valerate,
caproate,
heptanoate,
caprylate,
compared
to
studies
existing
literature.
Analysis
aroma
characteristics
molecular
docking
simulations
revealed
typical
profiles
mechanisms
various
mellow
develops
an
innovative
strategy
using
self-made
immobilized
lipases
catalyze
production
with
potential
applications
cosmetics.
LWT,
Год журнала:
2024,
Номер
194, С. 115813 - 115813
Опубликована: Фев. 1, 2024
This
study
delved
into
the
feasibility
of
fermenting
Rosa
roxburghii
Tratt
and
coix
seed
beverage
(RRTCSB)
using
dual-strain
fermentation.
The
impact
Trametes
versicolor
Lactiplantibacillus
plantarum
LB12
fermentation
on
RRTCSB's
physicochemical
properties
was
investigated.
Post-fermentation,
beverage's
color
clarified
brightened,
displaying
an
appealing
hue.
Even
concentrated
malic
acid,
which
contributes
both
sourness
astringency,
decreased
from
21.227
±
0.151
mg/mL
before
to
14.067
0.090
after
fermentation,
indicating
a
reduction
approximately
30%.
Regarding
characteristics,
retention
rate
vitamin
C
about
75%,
resulting
in
final
content
2.9
mg/mL.
Additionally,
polysaccharide
consistently
increased.
Specifically,
during
T.
experienced
approximate
72%
increase.
reached
2.85
Initially
declining
then
rising,
γ-aminobutyric
acid
(GABA)
increased
by
17%
GABA
5.12
mg/100
mL.
Fermentation,
particularly
with
versicolor,
gradually
enhanced
hypoglycemic
activity.
Ultimately,
this
successfully
demonstrated
introducing
L.
for
RRTCSB,
yielding
improved
novel
beverage.
