Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 8, 2024
Abstract
Mineralization
of
living
cells
represents
an
evolutionary
adaptation
that
enhances
cellular
resilience
to
physicochemical
stress.
Inspired
by
this
strategy,
we
have
here
developed
hybrid
materials
(HLMs),
incorporating
probiotics
into
mineralized
collagen
3D
matrices,
with
the
aim
protecting
and
promoting
successful
oral
delivery
bacteria.
Collagen
fibrils
are
simultaneously
self‐assembled
in
presence
(
Lactobacillus
acidophilus
,
La
was
used
as
model),
resulting
integration
matrix
(i.e.,
bulk
encapsulation).
During
process,
also
coated
a
nanofilm
apatite
mineral
(single‐cell
encapsulation),
which
provides
them
extra
protection
reinforces
their
viability
activity.
In
fact,
HLM
is
metabolically
active,
maintain
capacity
ferment
milk
yogurt
antibacterial
activity
against
two
major
foodborne
pathogens
Pseudomonas
aeruginosa
(Pa)
Staphylococcus
aureus
(Sa)
.
Interestingly,
additional
gastrointestinal
environment
simulated
gastric
fluid),
special
interest
for
healthcare
administration.
The
results
pave
way
creation
innovative
enhanced
functionalities
potential
produce
probiotic
foods
notable
antimicrobial
properties.
Foods,
Год журнала:
2025,
Номер
14(10), С. 1725 - 1725
Опубликована: Май 13, 2025
Probiotics
offer
numerous
health
benefits;
however,
preserving
their
viability
during
processing
and
storage
remains
a
major
challenge.
This
study
investigates
the
electrospinning
of
gelatin/dextran
(GE/DEX)
water-in-water
(W/W)
emulsions
for
Lactobacillus
plantarum
encapsulation.
By
varying
dextran
concentrations,
ways
in
which
phase
behavior,
viscosity,
conductivity
influence
fiber
formation
morphology
were
analyzed.
Scanning
transmission
electron
microscopy
confirmed
core-shell
nanofibers,
while
FT-IR
revealed
electrostatic
interactions
rather
than
chemical
reactions
between
GE
DEX.
Encapsulated
probiotics
exhibited
enhanced
under
thermal
stress
(65
72
°C),
(25
4
simulated
gastrointestinal
conditions,
maintaining
high
(>8
log
CFU/g)
compared
with
free
cells.
Notably,
gelatin-rich
shell
phases
provided
stronger
protection,
likely
due
to
gelation
properties
restricting
bacterial
mobility.
These
findings
demonstrate
that
W/W
is
an
effective
strategy
improve
probiotic
stability,
offering
potential
applications
functional
foods.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 8, 2024
Abstract
Mineralization
of
living
cells
represents
an
evolutionary
adaptation
that
enhances
cellular
resilience
to
physicochemical
stress.
Inspired
by
this
strategy,
we
have
here
developed
hybrid
materials
(HLMs),
incorporating
probiotics
into
mineralized
collagen
3D
matrices,
with
the
aim
protecting
and
promoting
successful
oral
delivery
bacteria.
Collagen
fibrils
are
simultaneously
self‐assembled
in
presence
(
Lactobacillus
acidophilus
,
La
was
used
as
model),
resulting
integration
matrix
(i.e.,
bulk
encapsulation).
During
process,
also
coated
a
nanofilm
apatite
mineral
(single‐cell
encapsulation),
which
provides
them
extra
protection
reinforces
their
viability
activity.
In
fact,
HLM
is
metabolically
active,
maintain
capacity
ferment
milk
yogurt
antibacterial
activity
against
two
major
foodborne
pathogens
Pseudomonas
aeruginosa
(Pa)
Staphylococcus
aureus
(Sa)
.
Interestingly,
additional
gastrointestinal
environment
simulated
gastric
fluid),
special
interest
for
healthcare
administration.
The
results
pave
way
creation
innovative
enhanced
functionalities
potential
produce
probiotic
foods
notable
antimicrobial
properties.
