Anti-atrophic Role of Ononis natrix subsp. hispanica Extract in Skeletal Muscle Atrophy: Pathway-targeted Insights and Experimental Validation
Food Bioscience,
Год журнала:
2025,
Номер
unknown, С. 106000 - 106000
Опубликована: Янв. 1, 2025
Язык: Английский
Decoding Chemical Profiles, Biological Functions, and Medicinal Properties of Liquidambar orientalis Extracts Through Molecular Modeling and Bioinformatic Methods
Food Frontiers,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 11, 2025
ABSTRACT
Liquidambar
orientalis
,
the
Anatolian
sweetgum
tree,
is
a
relict
and
endemic
species
in
Southwestern
Turkey,
traditionally
used
for
therapeutic
purposes.
Our
study
comprehensively
evaluated
potential
of
L.
extracts
from
its
aerial
parts
to
maximize
bioactive
compound
extraction
using
methanol,
ethyl
acetate,
water
as
solvents.
The
methanolic
extract
exhibited
highest
phenolic
(73.04
±
3.94
mg
gallic
acid
equivalent
[GAE]/g)
flavonoid
content
(48.86
0.76
rutin
[RE]/g),
demonstrating
superior
antioxidant
activity
2,2‐diphenyl‐1‐picrylhydrazyl
(DPPH)
(256.61
1.70
Trolox
[TE]/g),
2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic
acid)
(ABTS)
(308.41
3.14
TE/g),
cupric
ion–reducing
capacity
(CUPRAC)
(411.13
8.48
TE/g)
assays.
It
also
showed
significant
enzyme
inhibition
acetylcholinesterase
(4.43
0.09
galanthamine
[GALAE]/g),
tyrosinase
(149.16
1.14
kojic
[KAE]/g),
amylase
(0.93
0.02
mmol
acarbose
[ACAE]/g),
glucosidase
(1.60
0.01
ACAE/g),
suggesting
applications
neurodegenerative
disease
management,
skincare,
diabetes
treatment.
Furthermore,
methanol
displayed
promising
antimicrobial
due
compounds
such
chlorogenic
methyl‐3‐
O
‐caffeoyl
quinate.
potent
anticancer
effects
against
lung
cancer
(A549)
cells,
with
reductions
cell
viability
induction
autophagy.
aqueous
remarkable
efficacy
prostate
(PC3)
modulating
apoptosis
markers.
Breast
cells
(MDA‐MB‐231)
differential
responses,
acetate
promoting
extract–enhancing
molecular
docking
dynamics
simulations
provided
additional
evidence
supporting
key
phytochemicals
particularly
afzelin
epigallocatechin,
cancer‐related
targets
bacterial
enzymes.
Overall,
this
fills
gap
understanding
inhibitory
diverse
extracts,
highlighting
multi‐targeted
applications,
therapy.
Язык: Английский
Exploring the Utility of Prunus mahaleb Extracts as a Source of Natural Bioactive Compounds for Functional Applications
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(4)
Опубликована: Март 28, 2025
ABSTRACT
Prunus
mahaleb
has
garnered
attention
as
a
potent
medicinal
agent
and
functional
component.
We
aimed
to
detect
the
chemical
composition
biological
activities
of
several
parts
(fruit,
leaves,
twigs)
P.
.
Biological
were
assessed
for
antioxidant
properties,
enzyme
inhibition,
mutagenic/antimutagenic
effects,
antibacterial
efficacy.
Antioxidant
capabilities
evaluated
using
various
assays,
including
DPPH,
ABTS,
CUPRAC,
FRAP,
phosphomolybdenum,
metal
chelating.
The
constituents
extracts
identified
quantified
HPLC‐ESI‐MS/MS
method.
effects
inhibition
examined
on
some
enzymes,
AChE,
BChE,
tyrosinase,
amylase,
glucosidase.
Ames
test
was
used
evaluate
mutagenic
antimutagenic
properties
plant
extracts.
Furthermore,
broth
microdilution
assay
employed
possible
against
microorganisms.
methanol
extract
twigs
showed
superior
(DPPH:
388.39
mg
TE/g;
ABTS:
701.50
CUPRAC:
459.05
FRAP:
264.99
TE/g).
demonstrated
highest
tyrosinase
inhibitory
activity
(61.91
KAE/g).
A
total
40
metabolites,
mainly
flavonoids,
detected
through
analysis,
revealing
that
ferulic
acid,
naringenin,
herniarin
predominant
compounds.
In
test,
tested
exhibited
no
potential.
antimutagenicity
ethyl
acetate
from
leaves
particularly
efficient
frameshift
base
pair
substitution
mutations
induced
by
recognized
mutagens.
metabolic
activation
system
amplified
these
strong
rates
ranging
85%
98%.
results
indicated
effectiveness
at
dosages
between
6.25
0.195
mg/mL,
effective
Sarcina
lutea,
Bacillus
cereus
,
Candida
albicans
Staphylococcus
aureus
Our
findings
indicate
can
serve
versatile
raw
material
development
health‐promoting
applications,
medicines,
cosmeceuticals,
nutraceuticals.
Язык: Английский
Unlocking New Pharma/Nutraceutical Frontiers With Neuroprotective Properties of Three Hypericum Species: A Study Combination With In Vitro and In Silico Methodologies
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(4)
Опубликована: Апрель 1, 2025
ABSTRACT
This
study
investigates
the
phytochemical
composition
and
biopharmacological
potential
of
three
Hypericum
species
(
H.
scabrum
,
lysimachioides
uniglandulosum
)
from
Turkey.
Aqueous
hydroalcoholic
extracts
were
analyzed
for
their
total
phenolic
content
(TPC),
flavonoid
(TFC),
individual
components
(by
UHPLC–HRMS
technique).
Antioxidant
activities
investigated
by
DPPH,
ABTS,
CUPRAC,
FRAP,
phosphomolybdenum,
metal
chelating
assays.
The
inhibition
effects
tested
on
acetylcholinesterase
(AChE),
butyrylcholinesterase
(BChE),
tyrosinase,
amylase,
glucosidase
examined.
One
hundred
compounds
identified
in
chemical
composition,
specific
genus
such
as
hyperoside,
hypericin,
pseudohypericin,
detected.
highest
TPC
was
detected
ethanol/water
extract
with
69.21
mg
GAE/g.
Furthermore,
showed
strongest
free
radical
reducing
effect.
more
active
than
water
extracts.
Neuroprotective
assessments
indicated
downregulation
COX‐2
NOS‐2
genes
LPS‐stimulated
mouse
cortex
models,
alongside
modulation
SERT
NET
expression,
suggesting
reduced
neuroinflammation
enhanced
neurotransmitter
release.
Molecular
docking
dynamics
analyses
highlighted
strong
binding
interactions,
especially
NET_hyperoside
NET_myricitrin
complexes.
results
indicate
significant
therapeutic
these
extracts,
supporting
development
natural
agents
against
oxidative
stress,
neuroinflammation,
related
neurodegenerative
diseases.
Язык: Английский
The Biopotential of Bellardia trixago in Replacing Synthetic Compounds for Health‐Promoting Applications: Is It a Promising Candidate?
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Bellardia
trixago
(L.)
All.
(family
Orobanchaceae)
is
a
facultative
hemiparasitic
plant
used
traditionally
to
cure
many
diseases.
The
present
study
was
designed
evaluate
the
phenolic
constituents,
antioxidant,
enzyme
inhibitory,
and
cytotoxic
properties
of
aerial
parts
B.
.
Ethyl
acetate
(EtOAc),
ethanol
(EtOH),
70%
EtOH
extracts
were
prepared
by
maceration,
while
aqueous
extract
infusion.
Rutin,
4‐hydroxy
benzoic
acid,
ferulic
p
‐coumaric
hyperoside,
delphinidin
3,5
diglucoside,
kaempferol,
isoquercitrin
identified
in
most
with
variable
concentrations,
but
generally,
or
accumulated
highest
contents.
displayed
best
antiradical
(DPPH
=
13.55
mg
TE/g;
ATBS
53.78
TE/g)
ion‐reducing
(CUPRAC
50.09
FRAP
24.73
properties.
recorded
chelating
iron
power
(12.91
EDTAE/g)
EtOAc
exerted
total
antioxidant
activity
(2.29
2.49
mmol
TE/g,
>
0.05).
cholinesterase
(anti‐AChE
2.91
GALAE/g;
anti‐BchE
2.62
GALAE/g)
α‐glucosidase
(1.19
ACAE/g)
inhibition
from
extract,
that
against
α‐amylase
obtained
(0.69
ACAE/g).
toward
HT‐29
cell
line,
where
effect
(IC
50
38.42
μg/mL)
extract.
Furthermore,
network
pharmacology
analysis
revealed
critical
gene
targets
pathways
associated
bioactive
compounds.
Molecular
docking
studies
favorable
binding
modes
interaction
patterns
major
compounds
key
colon
cancer‐related
proteins,
which
further
supported
molecular
dynamics
simulations.
As
first
investigation
into
composition
pharmacological
effects
,
this
highlights
its
potential
as
promising
source
therapeutic
relevance
for
oxidative
stress‐related
conditions.
Язык: Английский
Lipophilic Extracts of Portulaca oleracea L.: Analysis of Bioactive Fatty Acids Targeting Microbial and Cancer Pathways
Pharmaceuticals,
Год журнала:
2025,
Номер
18(4), С. 587 - 587
Опубликована: Апрель 17, 2025
Background/Objectives:
Portulaca
oleracea
L.
(purslane)
is
a
widely
distributed
plant
known
for
its
medicinal
and
nutritional
properties.
This
study
aims
to
evaluate
the
fatty
acid
composition
bioactivities
of
crude
lipophilic
extracts
(chloroform/methanol
2:1)
from
purslane
collected
in
Serbia
Greece,
with
focus
on
antimicrobial
anticancer
potential.
Methods:
Chemical
analysis
was
conducted
determine
extracts.
Antibacterial
activity
assessed
using
standard
microdilution
assays,
while
antibiofilm
assays
evaluated
extracts’
ability
inhibit
biofilm
formation.
Cytotoxicity
tested
cancer
cell
lines
(MCF7,
HeLa,
CaCo2,
HepG2)
normal
keratinocyte
cells
(HaCaT).
Molecular
docking
dynamics
simulations
were
performed
explore
interactions
bioactive
acids
microbial
cancer-related
proteins.
Results:
The
revealed
significant
levels
polyunsaturated
acids,
linoleic
as
predominant
both
samples
(31.42%
34.51%).
Greek
extract
exhibited
stronger
antibacterial
than
Serbian
extract,
particularly
against
Aspergillus
versicolor,
Pseudomonas
aeruginosa,
Staphylococcus
aureus.
Antibiofilm
showed
up
89.54%
destruction
at
MIC
levels,
notable
reductions
exopolysaccharide
extracellular
DNA
production,
especially
samples.
testing
indicated
moderate
effects
(IC50
=
178.17–397.31
µg/mL)
being
non-toxic
keratinocytes.
identified
strong
between
key
Conclusions:
These
results
highlight
purslane’s
potential
source
compounds,
applications.
findings
suggest
that
could
be
developed
therapeutic
purposes
targeting
infections
cancer.
Язык: Английский