Totum-448, a polyphenol-rich plant extract, decreases hepatic steatosis and inflammation in diet-induced MASLD mice
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Abstract
The
increasing
prevalence
of
obesity-driven
metabolic
dysfunction-associated
steatotic
liver
disease
(MASLD)
urges
the
development
new
therapeutic
strategies.
Totum-448
is
a
unique
patented
combination
polyphenol-rich
plant
extracts
designed
to
reduce
hepatic
steatosis,
risk
factor
for
steatohepatitis
and
type
2
diabetes.
In
this
study,
we
investigated
effects
on
homeostasis
in
diet-induced
MASLD
mice.
For
purpose,
male
C57Bl6/J
mice
were
fed
high-fat
diet
with
sucrose-containing
drinking
water
12
weeks,
followed
by
supplementation
or
without
4
weeks.
Body
weight/composition,
caloric
intake,
plasma
parameters
whole-body
glucose
tolerance
measured
throughout
study
fecal
microbiota
composition
was
determined
16S
sequencing.
Hepatic
transcriptomic/lipidomic
profiles
immune
cell
assessed
histological/biochemical
assays,
RNA
sequencing,
MS-based
lipidomics,
spectral
flow
cytometry.
We
found
that
significantly
lowered
hyperinsulinemia
systemic
intolerance
affecting
body
weight,
fat
mass,
calorie
feces
production
composition.
Furthermore,
decrease
activity
score
macrovesicular
triglycerides
cholesterol
contents,
alanine
aminotransferase
levels
observed.
also
reduced
inflammatory
pro-fibrotic
transcriptomic
signatures
decreased
both
MASLD-induced
loss
tissue-resident
Kupffer
cells
recruitment
monocyte-derived
pro-inflammatory
macrophages.
Altogether,
reduces
steatosis
inflammation
insulin-resistant,
steatotic,
obese
mice,
dual
effect
likely
contributes
improved
homeostasis.
Supplementation
may
therefore
constitute
promising
novel
nutritional
approach
patients.
Язык: Английский
Interaction of starch nanoparticles with digestive enzymes and its effect on the release of polyphenols in simulated gastrointestinal fluids
Food Chemistry,
Год журнала:
2025,
Номер
472, С. 142883 - 142883
Опубликована: Янв. 11, 2025
Язык: Английский
Hepatoprotective effect of the Sechium HD-Victor hybrid extract in a model of liver damage induced by carbon tetrachloride in mice
Biomedicine & Pharmacotherapy,
Год журнала:
2025,
Номер
183, С. 117831 - 117831
Опубликована: Янв. 22, 2025
Язык: Английский
Effects of polyphenols on non-alcoholic fatty liver disease: a case study of resveratrol
Food & Function,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Plant
polyphenols
show
good
potential
for
preventing
chronic
metabolic
diseases,
especially
resveratrol,
by
reducing
insulin
resistance
and
fat
synthesis
linked
to
non-alcoholic
fatty
liver
disease.
Язык: Английский
The Effect of Quercetin on Non-Alcoholic Fatty Liver Disease (NAFLD) and the Role of Beclin1, P62, and LC3: An Experimental Study
Nutrients,
Год журнала:
2024,
Номер
16(24), С. 4282 - 4282
Опубликована: Дек. 11, 2024
Background/Objectives:
Non-alcoholic
fatty
liver
disease
(NAFLD)
is
a
major
metabolic
disorder
with
no
established
pharmacotherapy.
Quercetin,
polyphenolic
flavonoid,
demonstrates
potential
hepatoprotective
effects
but
has
limited
bioavailability.
This
study
evaluates
the
impact
of
quercetin
on
NAFLD
and
assesses
roles
autophagy-related
proteins
in
progression.
Methods:
Forty-seven
male
C57BL/6J
mice
were
fed
high-fat
diet
(HFD)
for
12
weeks
to
induce
NAFLD,
followed
by
treatment
4
weeks.
Mice
divided
into
baseline,
control,
two
groups,
receiving
low
(10
mg/kg)
high
(50
doses.
Liver
histology
was
scored
using
Activity
Score
(NAS).
Immunohistochemistry
immunoblotting
performed
analyze
autophagy
markers.
Results:
Quercetin-treated
groups
showed
significant
reductions
NAS
compared
controls
(p
=
0.011),
mainly
steatosis
steatohepatitis.
indicated
increased
expression
markers
LCA
p62
groups.
Western
blot
analysis
revealed
elevations
LC3A
treated
suggesting
improved
autophagic
activity
lipid
degradation.
Conclusions:
Quercetin
effectively
reduces
severity
modulates
proteins.
These
findings
suggest
that
enhances
flux,
supporting
its
therapeutic
NAFLD.
Additional
research
needed
clarify
molecular
mechanisms
determine
optimal
dosing
clinical
application.
Язык: Английский