Mlc1-Expressing Perivascular Astrocytes Promote Blood–Brain Barrier Integrity DOI Creative Commons
John E. Morales, Arpan De, Alexandra A. Miller

и другие.

Journal of Neuroscience, Год журнала: 2021, Номер 42(8), С. 1406 - 1416

Опубликована: Дек. 29, 2021

In the mammalian brain, perivascular astrocytes (PAs) closely juxtapose blood vessels and are postulated to have important roles in control of vascular physiology, including regulation blood–brain barrier (BBB). Deciphering specific functions for PAs BBB biology, however, has been limited by ability distinguish these cells from other astrocyte populations. order characterize selective vivo , a new mouse model generated which endogenous megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1) gene drives expression Cre fused mutated estrogen ligand-binding domain (Mlc1-T2A-CreERT2). This knock-in model, we term MLCT, allows identification tracking postnatal brain. We also demonstrate that MLCT-mediated ablation causes severe defects integrity, resulting premature death. PA loss results aberrant localization Claudin 5 -VE-Cadherin endothelial cell junctions as well robust microgliosis. Collectively, data reveal essential Mlc1-expressing regulating integrity mice indicate primary cause breakdown may contribute human neurologic disorders. SIGNIFICANCE STATEMENT Interlaced among billions neurons glia brain is an elaborate network vessels. Signals parenchyma unique permeability properties cerebral known However, understand very little about relative contributions different neural types functions. Here, show subpopulation leading junctions, breakdown, deficits.

Язык: Английский

Role of Vitronectin and Its Receptors in Neuronal Function and Neurodegenerative Diseases DOI Open Access

Yelizhati Ruzha,

Junjun Ni, Zhenzhen Quan

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(20), С. 12387 - 12387

Опубликована: Окт. 16, 2022

Vitronectin (VTN), a multifunctional glycoprotein with various physiological functions, exists in plasma and the extracellular matrix. It is known to be involved cell attachment, spreading migration through binding integrin receptor, mainly via RGD sequence. VTN also widely used maintenance expansion of pluripotent stem cells, but its effects go beyond that. Recent evidence shows more functions nervous system as it participates neural differentiation, neuronutrition neurogenesis, well regulating axon size, supporting guiding neurite extension. Furthermore, was proved play key role protecting brain can reduce permeability blood–brain barrier by interacting receptors vascular endothelial cells. Moreover, suggests that associated neurodegenerative diseases, such Alzheimer’s disease, function has not been fully understood. This review summarizes neurons describes diseases.

Язык: Английский

Процитировано

22
Astrocytic chloride regulates brain function in health and disease DOI Creative Commons
Verena Untiet

Cell Calcium, Год журнала: 2024, Номер 118, С. 102855 - 102855

Опубликована: Фев. 7, 2024

Chloride ions (Cl−) play a pivotal role in synaptic inhibition the central nervous system, primarily mediated through ionotropic mechanisms. A recent breakthrough emphathizes significant influence of astrocytic intracellular chloride concentration ([Cl−]i) regulation, field still its early stages exploration. Typically, [Cl−]i most animal cells is maintained at lower levels than extracellular [Cl−]o, critical balance to prevent cell swelling due osmotic pressure. Various Cl− transporters are expressed differently across types, fine-tuning [Cl−]i, while gradients utilised by several families channels. Although passive distribution within governed basic biophysical principles, astrocytes actively expend energy sustain much higher those achieved passively, and neuronal [Cl−]i. Beyond volume dynamically linked brain states influences signalling behaving animals. As vital component function, also plays development disorders where inhibitory transmission disrupted. This review synthesises latest insights into elucidating modulating function implications various pathophysiological conditions.

Язык: Английский

Процитировано

4
Laminins and the Blood-Brain Barrier DOI

Ava Nasrollahi,

Yao Yao

Matrix Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
TRPV4 in cerebral small vessel disease: A key interacting partner DOI Creative Commons
Sara Lambrichts,

Robert J. van Oostenbrugge,

Sébastien Foulquier

и другие.

Vascular Pharmacology, Год журнала: 2025, Номер unknown, С. 107492 - 107492

Опубликована: Март 1, 2025

Cerebral small vessel disease (cSVD) is a major cause of vascular cognitive impairment and dementia. The underlying mechanisms are centered around the dysfunction neurovascular unit include an blood-brain barrier (BBB) permeability, decreased cerebrovascular reactivity cerebral hypoperfusion. cells composing express wide variety mechanosensitive ion channels that relevant for these processes. Recent research has increasingly focused on mechanobiology microvessels with recent evidence pointing towards significant role transient receptor potential vanilloid 4 (TRPV4). This Ca2+-permeable channel regulates key physiological functions, including tone, angiogenesis, BBB integrity neuroinflammation. Beyond its role, implicates TRPV4 in pathological processes such as remodelling, impaired reactivity, dysfunction. In this review, we explore multiple roles within unit, interactions molecular partners, discuss contribution to cSVD.

Язык: Английский

Процитировано

0
Postnatal Development of Perivascular Astrocytic Processes and Detection of Local mRNA and Translation DOI

Katia Avila-Gutierrez,

Héloïse Monnet,

Philippe Mailly

и другие.

Methods in molecular biology, Год журнала: 2025, Номер unknown, С. 107 - 121

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Orchestrating the neuroglial compartment: Ontogeny and developmental interaction of astrocytes, oligodendrocytes, and microglia DOI
Imke Schuurmans, Annika Mordelt, Lot D. de Witte

и другие.

Handbook of clinical neurology, Год журнала: 2025, Номер unknown, С. 27 - 47

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Electroacupuncture Serum Alleviates Ogd/R-Induced Astrocyte Damage by Regulating the AQP4 Via m6A Methylation of lncRNA MALAT1 DOI
H. Zhang, Xiaomeng Xu, Xinying Li

и другие.

Neurochemical Research, Год журнала: 2025, Номер 50(2)

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
From energy metabolism to mood regulation: The rise of lactate as a therapeutic target DOI Creative Commons
Sen Zhang, Jie Xia,

Wenke He

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Neuroplastic Effect of Exercise Through Astrocytes Activation and Cellular Crosstalk DOI Creative Commons
Fengwu Li, Xiaokun Geng,

Ho Jun Yun

и другие.

Aging and Disease, Год журнала: 2021, Номер 12(7), С. 1644 - 1644

Опубликована: Янв. 1, 2021

Physical exercise is an effective therapy for neurorehabilitation.Exercise has been shown to induce remodeling and proliferation of astrocyte.Astrocytes potentially affect the recruitment function neurons; they could intensify responses neurons bring more process neuroplasticity.Interactions between astrocytes, microglia modulate neuroplasticity and, subsequently, neural circuit function.These cellular interactions promote number synapses, neurogenesis, cerebrovascular remodeling.However, roles crosstalk astrocytes with any subsequent neuroplastic effects have not studied extensively in exercise-induced settings.This article discusses impact physical on astrocyte highlights interplay neurons.The these cells may enhance neuroplasticity, leading exercise.

Язык: Английский

Процитировано

26
Astrocytes Transplanted during Early Postnatal Development Integrate, Mature, and Survive Long Term in Mouse Cortex DOI Creative Commons

Sabrina Chierzi,

J. Benjamin Kacerovsky,

Albert Hiu Ka Fok

и другие.

Journal of Neuroscience, Год журнала: 2023, Номер 43(9), С. 1509 - 1529

Опубликована: Янв. 20, 2023

Astrocytes have complex structural, molecular, and physiological properties form specialized microenvironments that support circuit-specific functions in the CNS. To better understand how astrocytes acquire their unique features, we transplanted immature mouse cortical into developing cortex of male female mice assessed integration, maturation, survival. Within days, developed morphologies acquired territories tiling behavior typical astrocytes. At 35-47 d post-transplantation, appeared morphologically mature expressed levels EAAT2/GLT1 similar to nontransplanted Transplanted also supported excitatory/inhibitory (E/I) presynaptic terminals within territories, displayed normal Ca2+ events. showed initially reduced expression aquaporin 4 (AQP4) at endfeet elevated EAAT1/GLAST, with both proteins showing normalized by 110 one year respectively. specific brain regions astrocytic integration cerebellum. Cortical interlaced Bergmann glia (BG) cerebellar molecular layer establish discrete territories. However, retained many features including higher EAAT2/GLT1, lower absence AMPAR subunit GluA1. Collectively, our findings demonstrate integrate, mature, survive (more than year) following transplantation retain properties. Astrocytic can be useful for investigating cell-autonomous (intrinsic) non-cell-autonomous (environmental) mechanisms contributing development/diversity, determining optimal timing transplanting cellular delivery or replacement regenerative medicine.SIGNIFICANCE STATEMENT The enable diverse structural remain understood. In this study, systematically analyzed early postnatal brain. We found cerebral during development integrate properties, show long-term survival vivo year). contrast, display altered ability more cerebellum, This study demonstrates developmental potential provides an approach tease apart determine phenotype

Язык: Английский

Процитировано

9