Astrocyte ensembles manipulated with AstroLight tune cue-motivated behavior

Nature Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

Язык: Английский

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A lactate-dependent shift of glycolysis mediates synaptic and cognitive processes in male mice

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 9, 2024

Astrocytes control brain activity via both metabolic processes and gliotransmission, but the physiological links between these functions are scantly known. Here we show that endogenous activation of astrocyte type-1 cannabinoid (CB1) receptors determines a shift glycolysis towards lactate-dependent production D-serine, thereby gating synaptic cognitive in male mice. Mutant mice lacking CB1 receptor gene astrocytes (GFAP-CB1-KO) impaired novel object recognition (NOR) memory. This phenotype is rescued by gliotransmitter its precursor L-serine, also lactate 3,5-DHBA, an agonist HCAR1. Such effect abolished when astrocyte-specific phosphorylated-pathway (PP), which diverts L-serine synthesis, blocked. Consistently, 3,5-DHBA promoted co-agonist binding site occupancy CA1 post-synaptic NMDA hippocampal slices PP-dependent manner. Thus, tight cross-talk astrocytic energy metabolism gliotransmission processes.

Язык: Английский

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Multiplex translaminar imaging in the spinal cord of behaving mice

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Март 21, 2023

While the spinal cord is known to play critical roles in sensorimotor processing, including pain-related signaling, corresponding activity patterns genetically defined cell types across laminae have remained challenging investigate. Calcium imaging has enabled cellular measurements behaving rodents but currently limited superficial regions. Here, using chronically implanted microprisms, we imaged sensory and motor-evoked regions at speeds inaccessible by other high-resolution techniques. To enable translaminar freely animals through additionally developed wearable microscopes with custom-compound microlenses. This system addresses multiple challenges of previous microscopes, their working distance, resolution, contrast, achromatic range. Using this system, show that dorsal horn astrocytes mice program-dependent lamina-specific calcium excitation. Additionally, tachykinin precursor 1 (Tac1)-expressing neurons exhibit acute mechanical pain not locomotion.

Язык: Английский

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Astrocyte structural heterogeneity in the mouse hippocampus

Glia, Год журнала: 2023, Номер 71(7), С. 1667 - 1682

Опубликована: Март 22, 2023

Abstract Astrocytes are integral components of brain circuits, where they sense, process, and respond to surrounding activity, maintaining homeostasis regulating synaptic transmission, the sum which results in behavior modulation. These interactions possible due their complex morphology, composed a tree‐like structure processes cover defined territories ramifying mesh‐like system fine leaflets unresolved by conventional optic microscopy. While recent reports devoted more attention dynamic with synapses, our knowledge about “backbone” physiological conditions is incomplete. Recent transcriptomic studies described astrocyte molecular diversity, suggesting structural heterogeneity regions such as hippocampus, crucial for cognitive emotional behaviors. In this study, we carried out analysis astrocytes across hippocampal subfields Cornu Ammonis area 1 (CA1) dentate gyrus dorsoventral axis. We found that display subfields, conserved along further appear contribute an exocytosis‐dependent manner signaling loop maintains backbone structure. findings reveal appears follow layer‐specific cues depend on neuro‐glial environment.

Язык: Английский

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Astrocytes adjust the dynamic range of cortical network activity to control modality-specific sensory information processing

Cell Reports, Год журнала: 2023, Номер 42(8), С. 112950 - 112950

Опубликована: Авг. 1, 2023

Cortical neuron-astrocyte communication in response to peripheral sensory stimulation occurs a topographic-, frequency-, and intensity-dependent manner. However, the contribution of this functional interaction processing inputs consequent behavior remains unclear. We investigate role astrocytes information at circuit behavioral levels by monitoring manipulating astrocytic activity vivo. show that control dynamic range cortical network activity, optimizing its responsiveness incoming inputs. The modulation contributes setting detection threshold for tactile thermal responses. mechanism such is mediated through inhibitory transmission adjust gain sensitivity responding networks. These results uncover maintaining an optimal associated with specific modalities.

Язык: Английский

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Astrocytes in memory formation and maintenance

Essays in Biochemistry, Год журнала: 2022, Номер 67(1), С. 107 - 117

Опубликована: Сен. 23, 2022

Learning and memory are fundamental but highly complex functions of the brain. They rely on multiple mechanisms including processing sensory information, formation, maintenance short- long-term memory, retrieval extinction. Recent experiments provide strong evidence that, besides neurons, astrocytes crucially contribute to these higher brain functions. However, interplay neurons in local neuron-glia assemblies is far from being understood. Although important basic cellular principles that govern link neuronal astrocytic have been established, additional clearly continue emerge. In this short essay, we first review current technologies allowing experimenter explore role behaving animals, with focus spatial memory. We then discuss signaling their learning also reveal gaps our knowledge currently prevent a comprehensive understanding how acquisition, storage by modulating circuits.

Язык: Английский

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Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Сен. 7, 2022

Astrocytes are key elements of brain circuits that involved in different aspects the neuronal physiology relevant to functions. Although much effort is being made understand how biology astrocytes affects circuits, astrocytic network heterogeneity and plasticity still poorly defined. Here, we have combined structural functional imaging astrocyte activity recorded mice using Ca2+-modulated photoactivatable ratiometric integrator specific optostimulation glutamatergic pathways map neuron-astrocyte circuitries nucleus accumbens (NAc). We showed pathway-specific responses induced by selective main inputs from prefrontal cortex, basolateral amygdala, ventral hippocampus. Furthermore, co-stimulation non-linear Ca2+-signaling integration, revealing integrative properties NAc astrocytes. All these results demonstrate existence NAc, providing an insight understanding integrates information.

Язык: Английский

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Gray and white matter astrocytes differ in basal metabolism but respond similarly to neuronal activity

Glia, Год журнала: 2022, Номер 71(2), С. 229 - 244

Опубликована: Сен. 5, 2022

Abstract Astrocytes are a heterogeneous population of glial cells in the brain, which adapt their properties to requirements local environment. Two major groups astrocytes protoplasmic residing gray matter as well fibrous white matter. Here, we compared energy metabolism cortex and corpus callosum representative regions, acute brain slices taking advantage genetically encoded fluorescent nanosensors for NADH/NAD + redox ratio ATP. presented more reduced basal ratio, lower cytosolic concentration ATP cortical astrocytes. In astrocytes, neurotransmitter glutamate increased extracellular concentrations K , typical correlates neuronal activity, induced ratio. While application decreased [ATP], combination resulted an increase levels. Strikingly, very similar regulation by was observed callosum. Finally, strong intrinsic activity provoked bicuculline withdrawal Mg 2+ caused shift state slight reduction [ATP] summary, shows distinct properties, but qualitatively responses probably reflecting different environment these regions.

Язык: Английский

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Thermodynamic model for memory

Biosystems, Год журнала: 2024, Номер 242, С. 105247 - 105247

Опубликована: Июнь 10, 2024

Язык: Английский

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Role of Astrocytes in Alzheimer’s Disease Pathogenesis and the Impact of Exercise-induced Remodeling

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 732, С. 150418 - 150418

Опубликована: Июль 17, 2024

Язык: Английский

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