RiboTag RNA Sequencing Identifies Local Translation of HSP70 In Astrocyte Endfeet After Cerebral Ischemia DOI Open Access

Bosung Shim,

Prajwal Ciryam,

Çiğdem Tosun

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Abstract Brain ischemia causes disruption in cerebral blood flow and blood-brain barrier (BBB) integrity which are normally maintained by the astrocyte endfeet. Emerging evidence points to dysregulation of translatome during ischemia, but its effects on endfoot unknown. In this study, we aimed investigate early a rodent model ischemia-reperfusion. To do so, immunoprecipitated astrocyte-specific tagged ribosomes (RiboTag IP) from mechanically isolated brain microvessels. mice subjected middle artery occlusion reperfusion contralateral controls, sequenced ribosome-bound RNAs perivascular endfeet identified 205 genes that were differentially expressed after ischemia. Pathways associated with differential expressions included proteostasis, inflammation, cell cycle, metabolism. Transcription factors whose targets enriched amongst upregulated translating HSF1, master regulator heat shock response. The most highly HSF1-dependent Hspa1a Hspa1b , encode inducible HSP70. We found HSP70 is coinciding an increase ubiquitination across proteome. These findings suggest robust proteostasis response proteotoxic stress Modulating may be strategy preserve function BBB ischemic stroke.

Язык: Английский

RiboTag RNA Sequencing Identifies Local Translation of HSP70 in Astrocyte Endfeet After Cerebral Ischemia DOI Open Access

Bosung Shim,

Prajwal Ciryam,

Çiğdem Tosun

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 309 - 309

Опубликована: Янв. 1, 2025

Brain ischemia causes disruption in cerebral blood flow and blood–brain barrier integrity, which are normally maintained by astrocyte endfeet. Emerging evidence points to dysregulation of the translatome during ischemia, but its effects on endfoot unknown. In this study, we aimed investigate early a rodent reperfusion model stroke. To do so, immunoprecipitated astrocyte-specific tagged ribosomes (RiboTag IP) from mechanically isolated brain microvessels. mice subjected middle artery occlusion contralateral controls, sequenced ribosome-bound RNAs perivascular endfeet identified 205 genes that were differentially expressed after ischemia. The main biological processes associated with these included proteostasis, inflammation, cell cycle/death, metabolism. Transcription factors whose targets enriched amongst upregulated translating HSF1, master regulator heat shock response. most highly HSF1-dependent Hspa1a Hspa1b, encode inducible HSP70. Using qPCR, Western blot, immunohistochemistry, confirmed HSP70 is This coincided an increase ubiquitination across proteome suggests induces proteostasis These findings suggest robust response proteotoxic stress Modulating may be strategy preserve function BBB integrity ischemic

Язык: Английский

Процитировано

0
Postnatal Development of Perivascular Astrocytic Processes and Detection of Local mRNA and Translation DOI

Katia Avila-Gutierrez,

Héloïse Monnet,

Philippe Mailly

и другие.

Methods in molecular biology, Год журнала: 2025, Номер unknown, С. 107 - 121

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
RiboTag RNA Sequencing Identifies Local Translation of HSP70 In Astrocyte Endfeet After Cerebral Ischemia DOI Open Access

Bosung Shim,

Prajwal Ciryam,

Çiğdem Tosun

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Abstract Brain ischemia causes disruption in cerebral blood flow and blood-brain barrier (BBB) integrity which are normally maintained by the astrocyte endfeet. Emerging evidence points to dysregulation of translatome during ischemia, but its effects on endfoot unknown. In this study, we aimed investigate early a rodent model ischemia-reperfusion. To do so, immunoprecipitated astrocyte-specific tagged ribosomes (RiboTag IP) from mechanically isolated brain microvessels. mice subjected middle artery occlusion reperfusion contralateral controls, sequenced ribosome-bound RNAs perivascular endfeet identified 205 genes that were differentially expressed after ischemia. Pathways associated with differential expressions included proteostasis, inflammation, cell cycle, metabolism. Transcription factors whose targets enriched amongst upregulated translating HSF1, master regulator heat shock response. The most highly HSF1-dependent Hspa1a Hspa1b , encode inducible HSP70. We found HSP70 is coinciding an increase ubiquitination across proteome. These findings suggest robust proteostasis response proteotoxic stress Modulating may be strategy preserve function BBB ischemic stroke.

Язык: Английский

Процитировано

0