Journal of Translational Medicine,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 9, 2025
The
partial
epithelial-mesenchymal
transition
(EMT)
is
emerging
as
a
significant
mechanism
in
diabetic
nephropathy
(DN).
LOX
copper
amine
oxidase
conventionally
thought
to
act
by
crosslinking
collagen.
However,
the
role
of
EMT
and
fibrotic
progression
has
not
been
investigated
experimentally.
bulk
RNA
sequencing
single-nuclei
(snRNA-seq)
analysis
were
explored
find
nephropathy.
We
then
possible
signaling
pathway
LOX,
both
vivo
vitro
inhibition
experiments
mice
HK-2
cells.
Besides,
we
further
assessed
kidney
fibrosis
renal
function.
expression
was
elevated
kidneys
mice.
Additionally,
snRNA-seq
results
indicated
that
higher
proximal
tubular
(PemtPT)
epithelial
Moreover,
found
increased
prompted
cells
(RTECs)
modulating
transcription
factor
Snail
vitro.
Remarkably,
effectively
mitigated
RTECs
mice,
thereby
attenuating
enhancing
identified
TGF-β
an
upstream
regulator
inhibiting
partially
reversed
program
induced
pathway.
Hyperglycemia
induces
via
TGF-β/LOX/Snail
axis,
contributing
fibrosis.
Inhibiting
can
reverse
RTECs,
diminish
fibrosis,
improve
Idiopathic
pulmonary
fibrosis
is
a
progressive
fibrotic
disease
characterized
by
excessive
deposition
of
(myo)fibroblast
produced
collagen
fibrils
in
alveolar
areas
the
lung.
Lysyl
oxidases
(LOXs)
have
been
proposed
to
be
central
enzymes
that
catalyze
cross-linking
fibers.
Here,
we
report
that,
while
its
expression
increased
lungs,
genetic
ablation
LOXL2
only
leads
modest
reduction
pathological
but
not
On
other
hand,
loss
another
LOX
family
member,
LOXL4,
markedly
disrupts
and
Furthermore,
knockout
both
Loxl2
Loxl4
does
offer
any
additive
antifibrotic
effects
when
compared
deletion
only,
as
LOXL4
deficiency
decreases
members
including
Loxl2.
basis
these
results,
propose
main
activity
underlying
lung
fibrosis.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Апрель 16, 2024
The
extracellular
matrix
(ECM)
is
a
complex
three-dimensional
structure
composed
of
proteins,
glycans,
and
proteoglycans,
constituting
critical
component
the
tumor
microenvironment.
Complex
interactions
among
immune
cells,
matrix,
cells
promote
development
metastasis,
consequently
influencing
therapeutic
efficacy.
Hence,
elucidating
these
interaction
mechanisms
pivotal
for
precision
cancer
therapy.
T
lymphocytes
are
an
important
system,
exerting
direct
anti-tumor
effects
by
attacking
or
releasing
lymphokines
to
enhance
effects.
ECM
significantly
influences
function
infiltration
within
microenvironment,
thereby
impacting
behavior
biological
characteristics
cells.
involved
in
regulating
synthesis,
degradation,
remodeling
through
secretion
cytokines
enzymes.
As
result,
it
affects
proliferation
invasive
ability
as
well
efficacy
immunotherapy.
This
review
discusses
underlying
lymphocyte-ECM
microenvironment
their
potential
application
It
provides
novel
insights
innovative
strategies
drug.
ACS Nano,
Год журнала:
2024,
Номер
18(32), С. 20861 - 20885
Опубликована: Июль 31, 2024
Liver
fibrosis
(LF)
is
a
pathological
repair
reaction
caused
by
chronic
liver
injury
that
affects
the
health
of
millions
people
worldwide,
progressing
to
life-threatening
cirrhosis
and
cancer
without
timely
intervention.
Due
complexity
LF
pathology,
multiple
etiological
characteristics,
deposited
extracellular
matrix,
traditional
drugs
cannot
reach
appropriate
targets
in
time-space
matching
way,
thus
decreasing
therapeutic
effect.
Nanoparticle
drug
delivery
systems
(NDDS)
enable
multidrug
co-therapy
develop
multifactor
strategies
targeting
processes,
showing
great
potential
therapy.
Based
on
pathogenesis
current
clinical
treatment
status
LF,
we
systematically
elucidate
mechanism
NDDS
used
LF.
Subsequently,
focus
progress
applications
for
including
combined
fibrotic
environment,
overcoming
biological
barriers,
precise
intracellular
regulation,
intelligent
responsive
microenvironment.
We
hope
this
review
will
inspire
rational
design
future
order
provide
ideas
methods
promoting
regression
cure.
Journal of Translational Medicine,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 9, 2025
The
partial
epithelial-mesenchymal
transition
(EMT)
is
emerging
as
a
significant
mechanism
in
diabetic
nephropathy
(DN).
LOX
copper
amine
oxidase
conventionally
thought
to
act
by
crosslinking
collagen.
However,
the
role
of
EMT
and
fibrotic
progression
has
not
been
investigated
experimentally.
bulk
RNA
sequencing
single-nuclei
(snRNA-seq)
analysis
were
explored
find
nephropathy.
We
then
possible
signaling
pathway
LOX,
both
vivo
vitro
inhibition
experiments
mice
HK-2
cells.
Besides,
we
further
assessed
kidney
fibrosis
renal
function.
expression
was
elevated
kidneys
mice.
Additionally,
snRNA-seq
results
indicated
that
higher
proximal
tubular
(PemtPT)
epithelial
Moreover,
found
increased
prompted
cells
(RTECs)
modulating
transcription
factor
Snail
vitro.
Remarkably,
effectively
mitigated
RTECs
mice,
thereby
attenuating
enhancing
identified
TGF-β
an
upstream
regulator
inhibiting
partially
reversed
program
induced
pathway.
Hyperglycemia
induces
via
TGF-β/LOX/Snail
axis,
contributing
fibrosis.
Inhibiting
can
reverse
RTECs,
diminish
fibrosis,
improve
