Advances in Fluorescent Probes of Non-alcoholic Fatty Liver Disease

Talanta, Год журнала: 2025, Номер 287, С. 127694 - 127694

Опубликована: Фев. 4, 2025

Язык: Английский

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Xiaohua Funing decoction ameliorates non-alcoholic fatty liver disease by modulating the gut microbiota and bile acids

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 10, 2025

Introduction The gut microbiota and bile acids (BAs) have emerged as factors involved in the development of non-alcoholic fatty liver disease (NAFLD). Xiaohua Funing decoction (XFD) is a traditional Chinese medicine formula used for treatment NAFLD. Previous studies indicated that XFD protects function, but underlying mechanism remains unclear. Methods In this study, Wistar rat model NAFLD (Mod) was established via high-fat diet. effects obeticholic acid (OCA) on Mod rats were subsequently evaluated. control (Con) group fed standard There eight each group, lasted 12 weeks. Furthermore, metagenomic sequencing BA metabolomic analyses performed. Results Compared to Con presented significant differences body weights; serum total cholesterol (TC) triglyceride (TG) levels; TG, TC, salt hydrolase levels ( p < 0.05 or 0.01). Importantly, OCA administration normalized these indicators Pathology white fat steatosis observed significantly alleviated groups abundances Bacteroidales_bacterium , Prevotella_ sp., bacterium_0.1xD8-71 unclassified_g_Turicibacter different from those 0.01), whereas abundance greater group. A 17, 24, 24 differentially abundant BAs detected feces, liver, samples groups, respectively serum, 16, 23, 14 BAs, respectively, including glycochenodeoxycholic acid, deoxycholic murideoxycholic lithocholic 23-nordeoxycholic 3β-ursodeoxycholic acid. addition, identified potential biomarker Discussion summary, our experiments revealed regulates providing beneficial lipid accumulation

Язык: Английский

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Differences in Fatty Acid Metabolism between MCDD and HFD Induced Metabolic Dysfunction-associated Fatty Liver Disease Model Mice

Biological Procedures Online, Год журнала: 2025, Номер 27(1)

Опубликована: Апрель 14, 2025

The global incidence of metabolic dysfunction-associated fatty liver disease (MAFLD) is increasing annually, which has become a major public-health concern. MAFLD typically associated with obesity, hyperlipemia, or syndrome. Dietary induction one the most common methods for preparing animal models MAFLD. However, there are phenotypic differences between methionine-choline-deficient diet (MCDD) and high fat (HFD) models. To explore in hepatic acid metabolism MCDD HFD induced MAFLD, we analyzed serum tissue from two We found that accumulation function damage were pathological features both Furthermore, model, expression transport proteins increased, while efflux mRNA decreased, along decrease blood lipid levels. In uptake proteins, an increase Impaired oxidation increased play key roles pathogenesis inverse changes de novo lipogenesis may represent important mechanism leads to

Язык: Английский

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Cth/Mpst double ablation results in early onset fatty liver disease in lean mice

Redox Biology, Год журнала: 2025, Номер unknown, С. 103641 - 103641

Опубликована: Апрель 1, 2025

Metabolic dysfunction-associated fatty liver disease (MAFLD), a condition that stems from hepatic lipid accumulation in the absence of damage and overt inflammation, has become most common disorder worldwide. Hydrogen sulfide (H2S), gasotrasmitter, endogenously generated mainly by cystathionine-γ lyase (CTH), cystathionine-β synthase (CBS) 3-mercaptopyruvate sulfurtransferase (MPST) enzymes, exhibits protective effect steatosis. Herein, we have demonstrated CTH MPST play central role MAFLD pathogenesis. Young Cth/Mpst knockout (Cth/Mpst-/-) mice, fed normal diet, had increased mass caused enhanced accumulation. Decreased insulin glucose sensitivity was observed CTH/MPST-deficient mice. At cellular level, CTH/MPST inhibition resulted deposition uptake hepatocytes. Transcriptome analysis revealed significant upregulation cholesterol biosynthesis SREBP-related genes Cth/Mpst-/- Transcription factor enrichment differentially expressed between two genotypes, major impact LXR, RXR PPARA phenotype. Sulfide donor (SG1002) treatment attenuated Our findings underline importance produced H2S pathogenesis introduce mouse as new animal model early onset

Язык: Английский

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A thiocoumarin based self-reporting sulfide prodrug strategy with a favorable safety profile

European Journal of Medicinal Chemistry, Год журнала: 2025, Номер 289, С. 117426 - 117426

Опубликована: Фев. 20, 2025

Язык: Английский

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