Modulation of NRF2/KEAP1 Signaling by Phytotherapeutics in Periodontitis
Antioxidants,
Год журнала:
2024,
Номер
13(10), С. 1270 - 1270
Опубликована: Окт. 18, 2024
Periodontitis
affects
up
to
40%
of
adults
over
60
years
old
and
is
a
consequence
gingivitis.
characterized
by
chronic
inflammation,
periodontal
damage,
alveolar
bone
resorption.
The
nuclear
factor
erythroid
2-related
2
(NFE2L2
or
NRF2)/Kelch-like
ECH-Associated
Protein
1
(KEAP1)
(NRF2/KEAP1)
signaling
pathway
plays
key
role
in
periodontitis
modulating
redox
balance
inflammation
the
periodontium.
However,
NRF2
expression
decreased
gingival
tissues
patients
with
while
oxidative
stress
significantly
increased
this
pathology.
Oxidative
lipopolysaccharide
(LPS)
produced
gram-negative
bacteria
favor
production
inflammatory
causing
favoring
bone.
In
review,
we
analyzed
current
literature
regarding
natural
synthetic
compounds
NRF2/KEAP1
vitro
vivo
models
order
evaluate
new
potential
treatments
that
can
improve
outcome
disease.
Язык: Английский
Core microbe Bifidobacterium in the hindgut of calves improves the growth phenotype of young hosts by regulating microbial functions and host metabolism
Microbiome,
Год журнала:
2025,
Номер
13(1)
Опубликована: Янв. 16, 2025
The
growth
and
health
of
young
ruminants
are
regulated
by
their
gut
microbiome,
which
can
have
lifelong
consequences.
Compared
with
subjective
grouping,
phenotypic
clustering
might
be
a
more
comprehensive
approach
to
revealing
the
relationship
between
calf
state
core
microbes.
However,
identification
beneficial
bacteria
its
internal
mechanisms
shaping
host
phenotype
differentiation
remains
unclear.
In
this
study,
calves
were
divided
into
two
clusters,
cluster1
cluster2,
based
on
29
indicators
using
cluster
analysis.
Calves
in
cluster2
showed
better
performance,
including
higher
body
weight
(BW),
average
daily
gain
(ADG),
dry
matter
intake
(DMI),
as
well
serum
high
level
total
superoxide
dismutase
(T-SOD),
interleukin-6
(IL-6),
insulin-like
factor-1
(IGF-1)
compared
those
cluster1.
Multi-omics
was
used
detect
microbial
features
among
different
clusters.
Distinct
differences
observed
clustered
microbiomes,
diversity
composition.
close
relationships
blood
metabolites,
microbiome
also
confirmed.
Bifidobacterium
members
dominant
contributors
metabolic
functions
abundance.
Furthermore,
pathways
involved
carbohydrate
degradation,
glycolysis,
biosynthesis
propionate
proteins
active,
while
methane
production
inhibited.
addition,
richness
hindgut
resistome
lower
than
isolation
culture
strain,
mice
experiment,
indicated
that
B.
longum
1109
from
feces
could
promote
hosts,
enhance
immunity
antioxidation,
improve
development
hindgut.
summary,
analysis
has
proved
feasible
reliable
for
identifying
subgroups
calves,
prompting
further
exploration
host-microbiome
interactions.
microbe
may
play
crucial
probiotic
role
differentiation.
This
study
enhances
our
comprehension
how
shapes
provides
new
insights
manipulation
colonizers
performance
productivity
ruminants.
Video
Abstract.
Язык: Английский
METTL3-mediated methylation of RAC2 contributes to cell motility, oxidative stress and inflammation in TNF‐α-stimulated rheumatoid arthritis fibroblast-like synovial cells
Journal of Orthopaedic Surgery and Research,
Год журнала:
2025,
Номер
20(1)
Опубликована: Фев. 20, 2025
Rheumatoid
arthritis
(RA)
is
a
widely
prevalent
rheumatic
condition
causing
joint
inflammation
and
damage.
RNA
methylation
plays
an
important
role
in
RA.
Herein,
we
intended
to
investigate
the
function
of
methyltransferase-like
3
(METTL3)
its
N6-methyladenosine
(m6A)
regulation
for
ras-related
C3
botulinum
toxin
substrate
2
(RAC2)
MH7A
cells
were
treated
with
TNF-α
establish
RA
cell
model.
The
expression
analysis
was
performed
by
RT-qPCR
western
blot.
Cellular
behaviors
examined
CCK-8
assay,
flow
cytometry,
wound
healing
assay
transwell
assay.
Oxidative
stress
assessed
detecting
associated
indicators.
Inflammatory
cytokines
measured
via
enzyme-linked
immunosorbent
(ELISA).
Interaction
between
METTL3
RAC2
analyzed
immunoprecipitation
(RIP)
MeRIP
highly
expressed
tissues
TNF-α-stimulated
cells.
Knockdown
enhanced
apoptosis
reduced
proliferation,
migration,
invasion
after
treatment.
downregulation
suppressed
oxidative
inflammatory
response
TNF-α-treated
promoted
through
m6A
methylated
modification,
METTL3/RAC2
could
activate
AKT
pathway.
overexpression
reversed
effects
knockdown
on
motility,
inflammation.
above
results
demonstrated
that
facilitated
progression
downregulating
dependent
mechanism
Язык: Английский
The double-edged sword of lncRNAs in rheumatoid arthritis: from controlling the disease to its progress
Clinical and Experimental Medicine,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 7, 2025
Rheumatoid
arthritis
(RA)
is
a
chronic
autoimmune
disease
characterized
by
inflammatory
responses
in
the
joints,
synovial
hyperplasia,
persistent
abnormal
proliferation
of
fibroblast-like
synoviocytes
(FLSs),
and
cartilage
erosion,
leading
to
joint
swelling
destruction.
The
underlying
mechanisms
this
entail
complex
interplay
factors,
with
long
noncoding
RNAs
(lncRNAs)
serving
as
main
contributors.
These
lncRNAs,
which
are
over
200
bp
length,
involved
regulating
responses,
damage,
FLS
growth.
Studies
have
shown
that
lncRNAs
dual
function
progression
RA,
they
can
both
promote
control
reduce
symptoms.
Nevertheless,
our
current
understanding
development
RA
incomplete,
exact
molecular
process
remain
unclear.
This
study
aims
elucidate
exert
their
inhibitory
stimulatory
effects,
well
explore
potential
diagnosing,
predicting
prognosis,
targeting
therapy
for
RA.
Язык: Английский
Mechanism of action of Nrf2 and its related natural regulators in rheumatoid arthritis
Journal of Orthopaedic Surgery and Research,
Год журнала:
2024,
Номер
19(1)
Опубликована: Ноя. 14, 2024
Rheumatoid
arthritis
(RA)
is
an
autoimmune
disease
characterized
by
synovitis
that
can
lead
to
joint
deformities.
To
date,
more
than
18
million
individuals
worldwide
have
been
diagnosed
with
RA,
making
it
one
of
the
most
prevalent
diseases
globally
and
posing
a
significant
threat
public
health
safety.
Due
complex
pathogenesis
disease,
which
involves
autoimmunity,
genetics,
inflammation
oxidative
stress
in
body's
tissues,
current
drug
therapy
generally
targets
single
molecule,
effective
efficient
drugs
involving
multiple
levels
are
lacking;
thus,
there
urgent
need
for
high-quality
research
treatment
this
field.
Nuclear
transcription
factor
erythroid
2-associated
2
(Nrf2)
plays
crucial
role
cellular
resistance
electrophilic
attacks
potential
pharmacological
target
chronic
treatment.
While
currently
no
Nrf2
approved
specifically
RA
treatment,
such
approach
holds
great
significance.
In
recent
years,
use
natural
products
treat
other
conditions
has
become
increasingly
widespread
because
their
superior
efficacy
minimal
side
effects.
Therefore,
article
provides
review
mechanism
summarizes
its
associated
pathways
aiming
offer
new
insights
strategies
prevention
management
RA.
Язык: Английский
Norisoboldine Reduces Arthritis Severity by Attenuating Inflammation, Oxidative Stress, and Extracellular Matrix Degradation in a Rat Model of Rheumatoid Arthritis
Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 8839 - 8852
Опубликована: Ноя. 1, 2024
Rheumatoid
arthritis
(RA)
is
a
chronic
autoimmune
disorder
marked
by
persistent
joint
inflammation,
pain,
and
tissue
degradation.
This
study
evaluates
the
therapeutic
potential
of
Norisoboldine
(NOR),
an
isoquinoline
alkaloid
from
Lindera
aggregata,
in
rat
model
RA.
Rats
were
divided
into
five
groups:
normal
control
(G1),
RA
(G2),
NOR-treated
groups
at
15
mg/kg
(G3)
30
(G4),
methotrexate-treated
group
(G5).
NOR's
anti-arthritic
effects
assessed
measuring
clinical
scores
inflammatory
markers
(RF,
CRP,
TNF-α,
IL-6,
IL-10).
Oxidative
stress
(MDA,
SOD,
catalase,
GPx)
pathways
(NF-κB/IKKβ
Nrf2/Keap1)
also
evaluated.
Histopathology
synovial
inflammation
NOR
treatment
significantly
reduced
severity,
as
evidenced
decreased
rats.
exhibited
strong
antioxidant
effects,
demonstrated
MDA
levels
enhanced
GPx
activities.
further
downregulated
matrix
metalloproteinases
(Mmp-2,
Mmp-3),
aggrecanases
(Adamts-4,
Adamts-5),
PCNA
expression.
confirmed
reductions
damage
groups.
These
findings
suggest
that
anti-inflammatory
properties
contribute
to
reducing
both
overall
severity
multifaceted
actions
support
its
novel
agent
for
demonstrates
protective
rats
oxidative
stress,
extracellular
degradation,
offering
promise
option
manage
pathology
comprehensively.
Язык: Английский