Acid-assisted polysaccharides extracted from Asparagus cochinchinensis protect against Alzheimer’s disease by regulating the microbiota-gut-brain axis DOI Creative Commons
Ruixue Li, Hui Wang, Qinjian Wang

и другие.

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Дек. 18, 2024

In this study, an acid-assisted extraction strategy was used to extract a novel polysaccharide (ACP) from Asparagus cochinchinensis, after which purified and subjected extensive characterization. ACP determined have average molecular weight of 15,580 Da in structural characterization studies, it found primarily consist glucose, galactose, L-fucose, fructose at 82.14:12.23:2.61:2.49 ratio. Trace amounts xylose, arabinose, rhamnose were also detected preparations 0.48:0.04:0.02 GC–MS analyses identified eight different sugar linkages within ACP, including Glc p -(1→, →2)-Glc →6)-Glc →4)-Glc →3, 4)-Glc →2,4) -Gal →4,6)-Gal →3,4,6)-Gal -(1 → present 23.70:1.30:3.55:50.77:6.91:1.10:11.50:1.18 molar percent ratios. One-dimensional NMR, two-dimensional methylation ultimately revealed that the is mainly composed →4)- β -D-Glc small amount→4,6)- α -D-Gal →3,4)- so on. Branched chain α-D-Glc 4)- connected residues 4)-β-D-Glc O-4 position or O-3 position. treatment SAMP8 mice associated with reductions oxidative stress brain pathology together enhanced cognitive function. treated presented increases Bacteroidota abundance reduced Firmicutes, Patescibacteria, Actinobacteriota, Campilobacterota abundance. Thus, can prevent Alzheimer’s disease by modulating microbe-gut-brain axis.

Язык: Английский

Resveratrol ameliorates ulcerative colitis by upregulating Nrf2/HO‑1 pathway activity: Integrating animal experiments and network pharmacology DOI Creative Commons

YU Xin-ya,

Xiaoxi Li,

Yunchun Xu

и другие.

Molecular Medicine Reports, Год журнала: 2024, Номер 29(5)

Опубликована: Март 14, 2024

Ulcerative colitis (UC) is a chronic idiopathic inflammatory condition affecting the rectum and colon. Inflammation compromisation of intestinal mucosal barrier are key in UC pathogenesis. Resveratrol (Res) naturally occurring polyphenol that exhibits anti‑inflammatory antioxidant properties. Nuclear factor erythroid‑2‑related 2/heme oxygenase 1 (Nrf2/HO‑1) pathway regulates occurrence development numerous types diseases through activity. However, it not clear whether Nrf2/HO‑1 involved treatment Res UC. Therefore, present study aimed to investigate modulates signaling attenuate mice. Dextran sulfate sodium (DSS) was used induce experimental male C57BL/6J Disease activity index (DAI) hematoxylin eosin (H&E) staning assessed magnitude colonic lesions ELISA) utilized quantify cytokines (IL‑6, IL‑1β, TNF‑α IL‑10) serum colon tissues. Immunohistochemistry Western blot were evaluate expression levels tight junction (TJ) proteins [zonula occludens (ZO)‑1 Occludin] Pharmacokinetic (PK) parameters derived from TCMSP database. Networkpharmacology employed identify biological function pharmacological mechanism process relieving UC, target screened. The binding ability verified by molecular docking. Finally, effectiveness substantiated blot. decreased DAI, ameliorated histopathological changes such as crypt loss, disappeatance epithelium, infiltration Additionally, pro‑inflammatory IL‑6, IL‑1β increased IL‑10 expression. also restored protein ZO‑1 occludin after DSS treatment, increasing integrity barrier. PK properties suggested possesses therapeutic potential for oral administration. Network pharmacology revealed alleviated pathways, confirmed Nrf2 has high affinity with against blotting demonstrated HO‑1. In conclusion, activated decrease clinical symptoms, responses, damage

Язык: Английский

Процитировано

8
Mechanism of Action and Therapeutic Implications of Nrf2/HO-1 in Inflammatory Bowel Disease DOI Creative Commons

Lingling Yuan,

Yingyi Wang, Na Li

и другие.

Antioxidants, Год журнала: 2024, Номер 13(8), С. 1012 - 1012

Опубликована: Авг. 20, 2024

Oxidative stress (OS) is a key factor in the generation of various pathophysiological conditions. Nuclear erythroid 2 (NF-E2)-related (Nrf2) major transcriptional regulator antioxidant reactions. Heme oxygenase-1 (HO-1), gene regulated by Nrf2, one most critical cytoprotective molecules. In recent years, Nrf2/HO-1 has received widespread attention as regulatory pathway for intracellular defense against oxidative stress. It considered potential target treatment inflammatory bowel disease (IBD). This review highlights mechanism action and therapeutic significance IBD complications (intestinal fibrosis colorectal cancer (CRC)), well phytochemicals targeting IBD. The results suggest that effects on mainly involve following aspects: (1) Controlling to reduce intestinal inflammation injury; (2) Regulation flora repair mucosal barrier; (3) Prevention ferroptosis epithelial cells. However, due complex role Nrf2/HO-1, more nuanced understanding exact mechanisms involved way forward future.

Язык: Английский

Процитировано

8
Resveratrol attenuates intestinal epithelial barrier dysfunction via Nrf2/HO‐1 pathway in dextran sulfate sodium‐induced Caco‐2 cells DOI Creative Commons

YU Xin-ya,

Yazhi Wang,

Yunchun Xu

и другие.

Immunity Inflammation and Disease, Год журнала: 2024, Номер 12(2)

Опубликована: Фев. 1, 2024

The intestinal tract serves as an innate barrier, safeguarding the internal milieu from microorganisms and toxins. Various inflammatory diseases have a strong association with barrier dysfunction. primary functional cells within tract, epithelial (IECs) their tight junctions (TJs), are crucial in preserving integrity of this mechanical barrier. Resveratrol (Res), plant-derived phenolic compound, exhibits range health-promoting benefits attributed to its anti-inflammatory properties. This study aims examine Res's efficacy bolstering IECs function.

Язык: Английский

Процитировано

7
Alleviating effect of methionine on intestinal mucosal injury induced by heat stress DOI
Shaohua Feng, Jie Zeng, Jia Li

и другие.

Journal of Thermal Biology, Год журнала: 2024, Номер 123, С. 103935 - 103935

Опубликована: Июль 1, 2024

Язык: Английский

Процитировано

4
Artemether Ameliorates Dss-Induced Ulcerative Colitis by Modulating Macrophage Polarization DOI
Xinxin Ren, Yi Li, Jia Xu

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Artemether ameliorates DSS-induced ulcerative colitis by modulating macrophage polarization DOI
Xinxin Ren, Yi Li, Jia Xu

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 161, С. 114991 - 114991

Опубликована: Июнь 5, 2025

Язык: Английский

Процитировано

0
Enhancing intestinal tight junction assembly by gallic acid as a subcellular basis for the pharmacological effect of Ocimum sanctum L. flower aqueous extract DOI Creative Commons

Wanapas Wachiradejkul,

Pichayapa Sukmak,

Supisara Treveeravoot

и другие.

Journal of Functional Foods, Год журнала: 2024, Номер 122, С. 106519 - 106519

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

2
Acid-assisted polysaccharides extracted from Asparagus cochinchinensis protect against Alzheimer’s disease by regulating the microbiota-gut-brain axis DOI Creative Commons
Ruixue Li, Hui Wang, Qinjian Wang

и другие.

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Дек. 18, 2024

In this study, an acid-assisted extraction strategy was used to extract a novel polysaccharide (ACP) from Asparagus cochinchinensis, after which purified and subjected extensive characterization. ACP determined have average molecular weight of 15,580 Da in structural characterization studies, it found primarily consist glucose, galactose, L-fucose, fructose at 82.14:12.23:2.61:2.49 ratio. Trace amounts xylose, arabinose, rhamnose were also detected preparations 0.48:0.04:0.02 GC–MS analyses identified eight different sugar linkages within ACP, including Glc p -(1→, →2)-Glc →6)-Glc →4)-Glc →3, 4)-Glc →2,4) -Gal →4,6)-Gal →3,4,6)-Gal -(1 → present 23.70:1.30:3.55:50.77:6.91:1.10:11.50:1.18 molar percent ratios. One-dimensional NMR, two-dimensional methylation ultimately revealed that the is mainly composed →4)- β -D-Glc small amount→4,6)- α -D-Gal →3,4)- so on. Branched chain α-D-Glc 4)- connected residues 4)-β-D-Glc O-4 position or O-3 position. treatment SAMP8 mice associated with reductions oxidative stress brain pathology together enhanced cognitive function. treated presented increases Bacteroidota abundance reduced Firmicutes, Patescibacteria, Actinobacteriota, Campilobacterota abundance. Thus, can prevent Alzheimer’s disease by modulating microbe-gut-brain axis.

Язык: Английский

Процитировано

1