GlycoPCT: Pressure Cycling Technology-Based Quantitative Glycoproteomics Reveals Distinctive N-Glycosylation in Human Liver Biopsy Samples of Nonalcoholic Fatty Liver Disease DOI
Wei Jiang, Min Liu, Tao Su

и другие.

Journal of Proteome Research, Год журнала: 2024, Номер 24(1), С. 202 - 209

Опубликована: Ноя. 27, 2024

Protein N-glycosylation is vital in the human liver and influences functions such as lipid metabolism, apoptosis, inflammation. However, site-specific patterns variations biopsy samples between healthy individuals those with nonalcoholic fatty disease (NAFLD) remain incompletely characterized, primarily due to limitations of current clinical glycoproteomic methods, including a large demand for samples, low efficiency tissue protein extraction, recovery rate intact N-glycopeptides (IGPs). To address this issue, we developed GlycoPCT, quantitative method based on pressure cycling technology. It enables efficient IGPs accurate analysis trace samples. Our research revealed total 4,459 unique 361 glycans from 758 glycoproteins. High-mannose type, complex fucosylation sialylation type N-glycans were significantly upregulated NAFLD group (p < 0.001, t test). Notably, also identified 182 67 proteins 0.05, FC > 1.50) 108 downregulated 44 0.67) group. Furthermore, highlighted an essential acute phase glycoprotein, alpha-1-acid glycoprotein 1 (A1TA), which synthesized plays significant role progression. These novel glyco-signatures provide crucial clues diagnosis pathogenesis NAFLD.

Язык: Английский

iMetaOmics: Advancing human and environmental health through integrated meta‐omics DOI Creative Commons
Chun‐Lin Shi, Tong Chen, Canhui Lan

и другие.

iMetaOmics., Год журнала: 2024, Номер 1(1)

Опубликована: Авг. 8, 2024

iMetaOmics is a quarterly international journal with the priority of publishing research work within scope One Health, featured well-designed omics. The also welcomes systematic integration extensive public datasets, new and meaningful perspectives, re-analysis high-impact data yielding different/valuable conclusions. microbiome plays critical roles in human [1], animal [2], plant nutrition health [3], food [4], environment [5], as well other aspects society (Figure 1). Metaomics, including metabarcoding, metagenomics, metatranscriptomics, metaproteomics, metabolomics, provide unique powerful tools to better understand taxonomy functions microbiome, biology, [6]. Aiming fill gap lacking top journals field Asia, iMeta Science Society its thousands Chinese scientist members found young but journal—iMeta (ISSN: 2770-5986; eISSN: 2770-596X). It has published more than 200 high-quality papers since 2022, original articles, reproducible methods protocols, systemic reviews peers updated novel findings, easy-to-use analytic tools, knowledge [7]. These publications include are not limited gut pertaining diseases [8], microbiota animals (e.g., chicken [9], pig [10], panda [11]), pan-cancer [12] pan-genome [13], soil associated diversity defense [14, 15], popular for metaomics analyses [16-18]. With great efforts contributions our stakeholders, like publisher, editorial board members, authors, reviewers, received first impact factor 23.7 2024, gradually accumulating broad diverse audiences readership, especially attracting explosive growth submissions recent days. To cope significantly increased submissions, we have from bimonthly journal. However, publication space maintaining high standard iMeta, still reject 90% although many them did an excellent work. Due success officially launching sister (eISSN: 2996-9514, ISSN: 2996-9506), further publish articles [19-23], [24-28], state-of-the-art methodologies [29, 30] satisfy increasing submission demand iMeta. Taking advantage same team, rejected manuscripts valuable/meaningful findings will be transferred iMetaOmics, together detailed comments reviewers. In this context, following review process shortened promote rapid publishing. Before official launch, 12 already been upon approval by authors issue. Currently, featuring reanalysis expected that establish reputation soon, attract wide range interest readers, contribute progress Human Environmental Health studies. Authors' own priority, while utilization analysis robust experimental validation can considered. Multiple evidence required support conclusion. New strategies advantage, indispensable. All used without reasonable concerns should publicly/temporally accessible reviewers editors. A video tutorial, software download, installation, operation, result displaying, highly recommended bioinformatic tools. Additionally, necessary ethics numbers, clear layouts, figures, significant expected. Chun-Lin Shi: Writing—original draft; writing—review editing. Tong Chen: Writing—review Canhui Lan: Ren-You Gan: Jun Yu: Fangqing Zhao: Yong-Xin Liu: editing; conceptualization. Zhao Yu hold position Editor-in-Chief iMetaOmics. Liu, Chen, Lan, Shi Gan Executive Editors

Язык: Английский

Процитировано

6
Glycosylation profiling of monkeypox virus structural proteins with poly Ser-Arg materials DOI
Gaohui Du, Cheng Chen,

Yun Cui

и другие.

The Analyst, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Glycosylation profiling of MPXV structural proteins with poly SR.

Язык: Английский

Процитировано

0
Deciphering N-Glycosylation Dynamics of Serum Monoclonal Immunoglobulins in Multiple Myeloma via EThcD-sceHCD-MS/MS DOI
Huixian Li,

Wanhong Lu,

Jin Qian

и другие.

Journal of Proteome Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Serum glycoprotein glycosylation changes can indicate disease onset and progression. However, the site-specific N-glycosylation of monoclonal immunoglobulins (M-proteins) in multiple myeloma (MM) its clinical implications are unclear. In this study, we isolated pathogenic micromonoclonal IgA or IgG (approximately 2 μg) from IgA-MM patients (n = 22) IgG-MM 30), normal polyclonal healthy controls (HCs) 16). Using EThcD-sceHCD-MS/MS, dynamics serum M-proteins MM were determined. Compared with IgA1 HCs, had higher fucosylation (58.1% vs 32.1%, p < 0.001), sialylation (68.0% 50.8%, 0.011), mannosylation (1.5% 0.3%, 0.001). While, IgG1 (97.8% 95.3%, addition, specific N-glycan abundances correlated features: for IgA1, HexNAc5Hex5Fuc1NeuAc1 was associated hypocomplementemia; IgG1, HexNAc4Hex3Fuc1 albumin level (r -0.363, 0.049) estimated glomerular filtration rate -0.433, 0.017); HexNAc4Hex5 therapeutic prognosis. conclusion, their isotypes HCs have distinct profiles, N-glycans characteristics

Язык: Английский

Процитировано

0
Clinical glycoproteomics: methods and diseases DOI Creative Commons
Yujia Wang,

Kaixin Lei,

Lijun Zhao

и другие.

MedComm, Год журнала: 2024, Номер 5(10)

Опубликована: Окт. 1, 2024

Abstract Glycoproteins, representing a significant proportion of posttranslational products, play pivotal roles in various biological processes, such as signal transduction and immune response. Abnormal glycosylation may lead to structural functional changes glycoprotein, which is closely related the occurrence development diseases. Consequently, exploring protein can shed light on mechanisms behind disease manifestation pave way for innovative diagnostic therapeutic strategies. Nonetheless, study clinical glycoproteomics fraught with challenges due low abundance intricate structures glycosylation. Recent advancements mass spectrometry‐based have improved our ability identify abnormal glycoproteins samples. In this review, we aim provide comprehensive overview foundational principles recent glycoproteomic methodologies applications. Furthermore, discussed typical characteristics, underlying functions, diseases, brain cardiovascular cancers, kidney metabolic Additionally, highlighted potential avenues future glycoproteomics. These insights provided review will enhance comprehension methods diseases promote elucidation pathogenesis discovery novel biomarkers targets.

Язык: Английский

Процитировано

3
GlycoPCT: Pressure Cycling Technology-Based Quantitative Glycoproteomics Reveals Distinctive N-Glycosylation in Human Liver Biopsy Samples of Nonalcoholic Fatty Liver Disease DOI
Wei Jiang, Min Liu, Tao Su

и другие.

Journal of Proteome Research, Год журнала: 2024, Номер 24(1), С. 202 - 209

Опубликована: Ноя. 27, 2024

Protein N-glycosylation is vital in the human liver and influences functions such as lipid metabolism, apoptosis, inflammation. However, site-specific patterns variations biopsy samples between healthy individuals those with nonalcoholic fatty disease (NAFLD) remain incompletely characterized, primarily due to limitations of current clinical glycoproteomic methods, including a large demand for samples, low efficiency tissue protein extraction, recovery rate intact N-glycopeptides (IGPs). To address this issue, we developed GlycoPCT, quantitative method based on pressure cycling technology. It enables efficient IGPs accurate analysis trace samples. Our research revealed total 4,459 unique 361 glycans from 758 glycoproteins. High-mannose type, complex fucosylation sialylation type N-glycans were significantly upregulated NAFLD group (p < 0.001, t test). Notably, also identified 182 67 proteins 0.05, FC > 1.50) 108 downregulated 44 0.67) group. Furthermore, highlighted an essential acute phase glycoprotein, alpha-1-acid glycoprotein 1 (A1TA), which synthesized plays significant role progression. These novel glyco-signatures provide crucial clues diagnosis pathogenesis NAFLD.

Язык: Английский

Процитировано

1