The sow vaginal and gut microbiota associated with longevity and reproductive performance

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 7, 2025

Abstract Background Sow longevity and reproductivity are essential in the modern swine industry. Although many studies have focused on genetic genomic factors for selection, little is known about associations between microbiome sows with reproduction. Results In this study, we collected sequenced rectal vaginal swabs from 48 sows, nine of which completed up to four parities (U4P group), exhibiting reproductive longevity. We first identified predictors sow rectum (e.g., Akkermansia ) vagina Lactobacillus U4P group using RandomForest early breeding stage parity. Interestingly, these bacteria showed decreased predicted KEGG gene abundance involved biosynthesis amino acids. Then, tracked longitudinal changes over sows. LEfSe analysis revealed parity-associated that existed both Streptococcus Parity 1, 2, Veillonella 4). also patterns bacterial change (d 0) d 110, such as , was all parties. Furthermore, potential better performance. Finally, discovered Prevotellaceae NK3B31 total number piglets born throughout vagina. Conclusions This study highlights how reproduction within parities. The identification parity-associated, pregnancy-related, performance-correlated provides foundation targeted modulation improve animal production.

Язык: Английский

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Characterisation of Proteus mirabilis isolates from the poultry production chain in Zhejiang Province, China: antimicrobial resistance, virulence factors and genotypic profiling

British Poultry Science, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Янв. 24, 2025

1. This study investigated antimicrobial resistance, virulence factors and genotypic profiling among Proteus mirabilis isolated from three sources (poultry farms, slaughterhouses retail markets) in the poultry production chain Zhejiang Province, China, to assess its potential risk public health.

Язык: Английский

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The Overall Quality Changes of Chinese Sauced Ducks at Different Stages During Processing and Storage

Foods, Год журнала: 2025, Номер 14(5), С. 834 - 834

Опубликована: Фев. 28, 2025

This study reveals the physicochemical, microbial, flavor, and sensory changes in sauced duck from marinating phase to end of storage, divided into six stages (stages A-F). The color, total plate count, volatile basic nitrogen, thiobarbituric acid reactive substance at different were clarified. We utilized 16S rRNA gene sequencing, GC-IMS, GC-MS explore bacterial flora, fatty composition, flavor characteristics. dominant bacteria identified A-C included Psychrobacter, Flavobacterium, Pseudomonas, while Lactobacillus Staphylococcus dominated during D-F. Aldehydes, esters, alcohols, ketones emerged as main compounds. Several unsaturated acids significantly (p < 0.05) decreased stage A F. quality improved. potential reactions determined, correlation analysis samples across was performed. 3-Methy-1-butanol could be a crucial indicator duck's overall quality. research support treatment optimization products.

Язык: Английский

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Investigating the correlation between phenotypes, adrenal transcriptome, and serum metabolism in laying ducks exhibiting varying behaviors under the same stressor

animal, Год журнала: 2024, Номер 18(11), С. 101343 - 101343

Опубликована: Сен. 24, 2024

Язык: Английский

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The Catalog of Microbial Genes and Metagenome-Assembled Genomes from the Gut Microbiomes of Five Typical Crow Species on the Qinghai–Tibetan Plateau

Microorganisms, Год журнала: 2024, Номер 12(10), С. 2033 - 2033

Опубликована: Окт. 8, 2024

While considerable progress has been made in understanding the complex relationships between gut microbiomes and their hosts, especially mammals humans, functions of these microbial communities avian species remain largely unexplored. This gap knowledge is particularly notable, given critical roles are known to play facilitating crucial physiological functions, such as digestion, nutrient absorption, immune system development. Corvidae birds omnivorous widely distributed across various habitats, exhibiting strong adaptability often displaying traits accompanying humans. However, date, information on composition, sequenced genomes, functional characteristics crow microbes lacking. Herein, we constructed first relatively comprehensive crows gene catalog (2.74 million genes) 195 high-quality medium-quality metagenome-assembled genomes using 53 metagenomic samples from five typical (

Язык: Английский

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Metagenome-Assembled Genomes of Pig Fecal Samples in Nine European Countries: Insights into Antibiotic Resistance Genes and Viruses

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2409 - 2409

Опубликована: Ноя. 24, 2024

Gut microbiota plays a crucial role in the health and productivity of pigs. However, spread antibiotic resistance genes (ARGs) viruses within pig intestinal poses significant threats to animal public health. This study utilized 181 samples from nine European countries employed metagenomic assembly methods investigate dynamics distribution ARGs microbiota, aiming observing their associations with potential bacterial hosts. We identified 4605 metagenome-assembled genomes (MAGs), corresponding 19 phyla, 97 families, 309 genera, total 449 species. Additionally, 44 MAGs were classified as archaea. Analysis revealed 276 ARG types across 21 classes, Glycopeptide being most abundant class, followed by class Multidrug.

Язык: Английский

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Linking growth performance and carcass traits with enterotypes in Muscovy ducks

Animal Bioscience, Год журнала: 2024, Номер 37(7), С. 1213 - 1224

Опубликована: Апрель 25, 2024

Objective: Enterotypes (ETs) are the clustering of gut microbial community structures, which could serve as indicators growth performance and carcass traits. However, ETs have been sparsely investigated in waterfowl. The objective this study was to identify ileal explore correlation with traits Muscovy ducks.Methods: A total 200 ducks were randomly selected from a population 5,000 at 70-day old, weighed slaughtered. traits, including body weight, dressed weight evidenced percentage, percentage apparent yield, breast muscle leg muscle, determined. contents ileum collected for isolation DNA 16S rRNA gene sequencing. identified based on sequencing data performed by Spearman analysis.Results: Three (ET1, ET2, ET3) observed microbiota significant differences number features α-diversity among these (p<0.05). Streptococcus, Candida Arthritis, Bacteroidetes presentative genus ET1 ET3, respectively. Correlation analysis revealed that Lactococcus Bradyrhizobium significantly correlated eviscerated yield (p<0.05) while found close association ducks. different did not show difference (p>0.05). be yield.Conclusion: Our findings substantial variation associated It is implied might potential valuable biomarker assessing duck would provide novel insights into interaction

Язык: Английский

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A 16S RNA Analysis of Yangzhou Geese with Varying Body Weights: Gut Microbial Difference and Its Correlation with Body Weight Parameters

Animals, Год журнала: 2024, Номер 14(14), С. 2042 - 2042

Опубликована: Июль 12, 2024

China is a major goose-raising country, and the geese industry plays significant role in animal husbandry. Therefore, goose growth performance (body weight) critical topic. Goose gut microbiota influences weight gain by regulating its energy metabolism digestion. Additionally, impact of cecal microbial community structure on development, metabolism, immunity has been examined. However, most studies have used different additives or feeds as variables. Improving understanding dynamic changes communities body weights during their development correlation with host’s necessary. In this study, healthy Yangzhou large (L) small (S) weights, all at same age (70 days old) under feeding conditions, were sequenced using 16S rRNA. The sequencing results annotated QIIME2 (classify-sklearn algorithm) software, linkET package was to explore between intestinal microorganisms (Spearman). At phylum level, Firmicutes/Bacteroidetes ratio group approximately 20% higher than that group, Bacteroidetes Firmicutes exhibiting highly negative correlation. genus Bacteroides constituted abundant both groups, although Prevotellaceae_Ga6A1_group exhibited abundance group. Spearman analysis analyze microflora production indicators showed differences two groups birth significantly positively correlated Deferribacterota level. leg chest muscle positive correlations Prevotellace-ae_Ga6A1_group, suggesting promoting muscles. A observed [Ruminococ-cus]_torque Prevotellaceae_Ga6A1_group. These findings offer crucial theoretical foundation for study gastrointestinal provide insights into formulation poultry probiotics.

Язык: Английский

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Unraveling the gut microbiota of Tibetan chickens: insights into highland adaptation and ecological advantages

Microbiology Spectrum, Год журнала: 2024, Номер unknown

Опубликована: Сен. 30, 2024

ABSTRACT Tibetan animals have several unique advantages owing to the harsh ecological conditions under which they live. However, compared mammals, understanding of and underlying mechanisms representative high-latitude bird, chicken ( Gallus gallus , TC), remains limited. The gut microbiota has been conclusively shown be closely related both host health environmental adaptation. This study aimed explore relationships between cecal microbiome TCs based on comparisons among three populations: native residing plateau, domestic living in plain, one plain species. Metatranscriptomic sequencing revealed a significant enrichment active Bacteroidetes but loss Firmicutes TCs. Additionally, upregulated expression genes showed enriched pathways energy metabolism, glycan immune response. Furthermore, involved biosynthesis short-chain fatty acids (SCFAs) secondary bile (SBAs) was Data from targeted metabolomics further confirmed elevated levels certain SCFAs SBAs cecum Based multi-omics association analysis, we proposed that higher ratio Bacteroidetes/Firmicutes may attributed efficient metabolism stronger immunological activity Our findings provide better interactions highland adaptation, novel insights into by chickens adapt plateau hypoxic environment. IMPORTANCE composition function were significantly different population population. Higher response found exhibited acids, resulting content these metabolites. contribute high-altitude adaptive advantage

Язык: Английский

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Effect of Melatonin on the Production Performance, Blood Biochemical Parameters, Nutrient Digestibility, and Gastrointestinal Microbiome of Liaoning Cashmere Goats

Agriculture, Год журнала: 2024, Номер 14(11), С. 1983 - 1983

Опубликована: Ноя. 5, 2024

Melatonin’s capacity to improve cashmere production and quality in goats is well established, but its underlying mechanisms, particularly those concerning the gastrointestinal microbiome, remain inadequately understood. This study aims elucidate effects of melatonin implantation on performance, blood biochemical parameters, nutrient digestibility, microbiome Liaoning goats. Thirty newborn goat lambs were selected randomly assigned control groups using a paired test design. The group received three implantations at 15, 75, 135 days age, respectively, with dosage 2 mg/kg body weight, while no treatment. Digestive metabolism tests conducted 150 300 age; prior these tests, blood, rumen fluid, rectal feces collected. Apparent digestibility indexes determined, fluid analyzed microbial 16S rRNA sequencing. results indicated that significantly reduced daily weight gain 60 (p < 0.05) increasing 0.05). Additionally, it increased length fineness Melatonin enhanced nitrogen deposition 0.05), elevated plasma levels T-AOC, CAT, GSH-PX, BUN MDA, GOT, GPT, AKP Moreover, Ace Chao1 indices genera beneficial for feed digestion absorption, including Prevotella, Lachnospiraceae, Ruminococcus, Synergistaceae 0.05); abundance positively correlated improved antioxidant activity, liver kidney function. In conclusion, enhances by modulating microbiota, function, provides theoretical foundation melatonin’s role microbiota modulation, which essential promoting high-quality sustainable development industry.

Язык: Английский

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