bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 12, 2024
Abstract
The
mode
of
actions
antibiotics
can
be
broadly
classified
as
bacteriostatic
and
bactericidal.
leads
to
the
arrested
growth
cells
while
bactericidal
causes
cell
death.
In
this
work,
we
report
applicability
Deuterium
stable
isotope
probing
(DSIP)
in
combination
with
Raman
spectroscopy
(Raman
DSIP)
for
discrimination
among
on
basis
their
action
at
community
level.
We
optimized
concentration
deuterium
oxide
required
metabolic
activity
monitoring
without
compromising
microbial
growth.
also
identified
a
novel
carbon-deuterium
qualitative
spectral
marker
biofingerprint
region.
This
used
early
identification
antibiotic’s
action.
Our
results
explores
new
perspective
which
supports
utility
based
vibrational
tags
field
clinical
spectroscopy.
Understanding
bacterial
short
objective
manner
significantly
enhance
management
abilities
infectious
diseases
may
help
personalised
antimicrobial
therapy.
Figure
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 10, 2025
The
ubiquitous
use
of
plastics
demands
thoughtfulness
about
their
fate
in
the
environment.
Biodegradability
is,
therefore,
a
prerequisite
for
future
many
applications,
including
agriculture.
Here,
we
bring
forward
stable
isotope
(resonance)
Raman
microspectroscopy
at
single-cell
level
to
broaden
mechanistic
understanding
microbial
degradation
(micro)plastics
natural
systems.
We
selected
perdeuterated
d-polylactic
acid
(dPLA)
as
model
plastic,
synthesized
from
d-lactic
acid-d4,
via
enantioselective,
biocatalytic
reduction
pyruvate-d3.
With
dPLA
hand,
traced
deuterium
label
during
incubation
experiments
into
biomass
using
C–D
vibrations
(appear
Raman-silent
region
undeuterated
biomass,
2050–2300
cm–1).
2068
cm–1
band
was
indicative
strongly
deuterated
lipids
enabling
detection
metabolic
differences
with
(i.e.,
stronger
lipid
and
weaker
protein
deuteration)
compared
glucose-d12
D2O
alternative
D
sources.
Single-cell
analysis
key
detecting
phenotypic
heterogeneity
classifying
cells
naturally
occurring
bacterium
Sphingomonas
koreensis
two
clusters:
one
showed
significantly
deuteration
than
Escherichia
coli
control,
whereas
other
nonlabeled.
could
even
be
detected
strong
resonance
signal
carotenoids,
highlighting
potential
high
throughput
technologies
like
imaging
cell
sorting.
To
further
demonstrate
transferability
environmental
samples,
experiment
repeated
soil
bacteria
isolates,
uptake
observed
after
2
weeks.
The Analyst,
Год журнала:
2024,
Номер
149(10), С. 2833 - 2841
Опубликована: Янв. 1, 2024
Sensing
and
visualization
of
metabolites
metabolic
pathways
in
situ
are
significant
requirements
for
tracking
their
spatiotemporal
dynamics
a
non-destructive
manner.
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology,
Год журнала:
2023,
Номер
16(1)
Опубликована: Июль 30, 2023
Abstract
Protein
unfolding
and
aggregation
are
often
correlated
with
numerous
diseases
such
as
Alzheimer's,
Parkinson's,
Huntington's,
other
debilitating
neurological
disorders.
Such
adverse
events
consist
of
a
plethora
competing
mechanisms,
particularly
interactions
that
control
the
stability
cooperativity
process.
However,
it
remains
challenging
to
probe
molecular
mechanism
protein
dynamics
aggregation,
monitor
them
in
real‐time
under
physiological
conditions.
Recently,
Raman
spectroscopy
its
plasmon‐enhanced
counterparts,
surface‐enhanced
(SERS)
tip‐enhanced
(TERS),
have
emerged
sensitive
analytical
tools
potential
perform
studies
functional
groups
showing
significant
promise
probing
related
aggregation.
We
summarize
fundamental
working
principles
Raman,
SERS,
TERS
nondestructive,
easy‐to‐perform,
fast
for
Finally,
we
highlight
utility
these
techniques
analysis
vibrational
spectra
proteins
from
various
sources
tissues,
pathogens,
food,
biopharmaceuticals,
lastly,
biological
fouling
retrieve
precise
chemical
information,
which
can
be
potentially
translated
practical
applications
point‐of‐care
(PoC)
devices.
This
article
is
categorized
under:
Therapeutic
Approaches
Drug
Discovery
>
Emerging
Technologies
Diagnostic
Tools
Nanodevices
Nanotechnology
Biology
Nanoscale
Systems
Journal of Biophotonics,
Год журнала:
2023,
Номер
17(2)
Опубликована: Ноя. 28, 2023
Abstract
Global
proteome
changes
in
microbes
affect
the
survival
and
overall
production
of
commercially
relevant
metabolites
through
different
bioprocesses.
The
existing
methods
to
monitor
level
are
destructive
nature.
Stable
isotope
probing
(SIP)
coupled
with
Raman
spectroscopy
is
a
relatively
new
approach
for
analysis.
However,
applying
this
monitoring
large
culture
volume
not
cost‐effective.
In
study,
first
time
we
presenting
novel
method
combining
reverse
SIP
using
13
C‐glucose
Deuterium
spectroscopy.
findings
study
revealed
visible
(blue
shifts)
related
peaks
that
can
be
used
dynamics,
is,
synthesis
nascent
amino
acids
its
turnover
non‐destructive,
cost‐effective,
label‐free
manner.
Our
research
is
focused
on
evaluating
the
potentials
of
Raman
reverse
stable
isotope
probing
for
sensing
and
visualizing
Shikimate
pathway
activity
in
prokaryotic
eukaryotic
model
systems
a
cost-effective
non-destructive
manner
at
community
single
cell
level
situ.
In
this
work,
we
have
successfully
demonstrated
that
by
using
unlabeled
12C-glucose
as
sole
carbon
source
13C
labelled
microbial
culture
medium
can
be
monitored
qualitatively
quasi-quantitatively.
The
Pathway
links
central
metabolism
to
biosynthesis
aromatic
ring
containing
metabolites
such
amino
acids,
folates,
salicylic
acid,
others.
incorporation
12C
metabolome
pool
causes
blue
shift
spectra.
Phenylalanine
was
selected
representative
band
shikimate
marker.
Temporal
changes
intensity
bands
over
period
0
24
hours
been
analyzed
demonstrate
feasibility
approach.
We
also
visualized
phenylalanine
turnover
hyperspectral
imaging
aided
multivariate
curve
resolution.
novelty
our
study
lies
fact
first
time
has
situ
cost-effective,
extraction
free,
manner.
overall
findings
are
very
encouraging
support
prospective
utility
approach
monitoring
pathways
related
rings
metabolites.
This
wide
range
applications
commercially
medically
relevant
ACS Omega,
Год журнала:
2024,
Номер
9(22), С. 23753 - 23760
Опубликована: Май 22, 2024
The
mode
of
action
antibiotics
can
be
broadly
classified
as
bacteriostatic
and
bactericidal.
leads
to
the
arrested
growth
cells,
while
bacteriocidal
causes
cell
death.
In
this
work,
we
report
applicability
deuterium
stable
isotope
probing
(DSIP)
in
combination
with
Raman
spectroscopy
(Raman
DSIP)
for
discriminating
at
community
level.
Photochem,
Год журнала:
2024,
Номер
4(4), С. 417 - 433
Опубликована: Сен. 26, 2024
Surface-enhanced
Raman
scattering
(SERS)
is
a
powerful
tool
for
biosensing
with
high
sensitivity,
selectivity,
and
capability
of
multiplex
monitoring
both
in
vivo
vitro
studies.
This
has
been
applied
the
identification
detection
different
biological
metabolites
such
as
lipids,
nucleic
acids,
proteins.
The
present
review
article
explores
vast
applications
metallic
nanoparticles
SERS-based
biosensing.
We
have
summarized
discussed
fundamental
principles,
theories,
developments,
challenges,
perspectives
field
using
metal
nanoparticle
substrates
namely
gold,
silver,
copper,
bimetallic
nanoparticles.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(35), С. 45888 - 45900
Опубликована: Авг. 20, 2024
Photoinduced
enhanced
Raman
spectroscopy
(PIERS)
has
emerged
as
an
efficient
technique
for
enhancing
the
vibrational
modes
of
analyte
molecules
adsorbed
on
a
plasmonic
nanoparticle-semiconductor
hybrid
material
through
chemical
enhancement
governed
by
electron
transfer
from
semiconductor
to
nanoparticles
under
additional
ultraviolet
(UV)
preirradiation
step.
The
increase
in
is
imperative
analyzing
and
detecting
pharmaceutically
important
moieties,
such
amino
acids
proteins,
with
low
scattering
cross
section,
even
complex
biological
environments.
Herein,
we
demonstrate
that
UV
induced
creation
oxygen
vacancies
introducing
concentration
(≈1%)
Ni
dopant
2D
platelike
morphology
BiOCl
semiconductor;
i.e.,
defect
states
can
induce
charge
nanoparticles.
This
phenomenon
facilitates
surface.
Additionally,
have
shown
usefulness
this
method
protein
immobilization
substrate
surface,
followed
identification
specific
mixture
proteins.
Proteins
containing
cysteine
residues
capture
these
electrons
form
surface-bound
thiol
group
via
transient
disulfide
adduct
radical.
allows
differential
binding
depending
surface
Through
PIERS
principal
component
analysis,
possibility
probing
distinguishing
biomolecules
based
their
composition
secondary
structure
components
mixtures,
thus
paving
way
analysis
systems.
