Digital Surface-Enhanced Raman Spectroscopy–Lateral Flow Test Dipstick: Ultrasensitive, Rapid Virus Quantification in Environmental Dust

Environmental Science & Technology, Год журнала: 2024, Номер 58(11), С. 4926 - 4936

Опубликована: Март 7, 2024

This study introduces a novel surface-enhanced Raman spectroscopy (SERS)-based lateral flow test (LFT) dipstick that integrates digital analysis for highly sensitive and rapid viral quantification. The SERS–LFT dipsticks, incorporating gold–silver core–shell nanoparticle probes, enable pixel-based of large-area SERS scans. Such an approach enables ultralow-level detection viruses readily distinguishes positive signals from background noise at the pixel level. developed SERS–LFTs demonstrate limits (LODs) 180 fg SARS-CoV-2 spike protein, 120 nucleocapsid 7 plaque forming units intact virus, all within <30 min. Importantly, methods maintain their robustness LODs in presence indoor dust, thus underscoring potential accurate reliable virus diagnosis quantification real-world environmental settings.

Язык: Английский

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Sleep condition detection and assessment with optical fiber interferometer based on machine learning

iScience, Год журнала: 2023, Номер 26(7), С. 107244 - 107244

Опубликована: Июнь 30, 2023

The prevalence of sleep disorders has increased because the fast-paced and stressful modern lifestyle, negatively impacting quality human life work efficiency. It is crucial to address problems. However, current practice diagnosing using polysomnography (PSG) limitations such as complexity, large equipment, low portability, hindering its practicality for daily use. To overcome these challenges, in this article an optical fiber sensor proposed a viable solution monitoring. This device offers benefits like power consumption, non-invasiveness, absence interference, real-time health We introduce with interferometer capture ballistocardiography (BCG) electrocardiogram (ECG) signals from body. Furthermore, new machine learning method condition detection. Experimental results demonstrate superior performance architecture model monitoring assessing quality.

Язык: Английский

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表面增强拉曼光谱技术在流行性病毒检测领域的研究进展

Chinese Journal of Lasers, Год журнала: 2024, Номер 51(9), С. 0907006 - 0907006

Опубликована: Янв. 1, 2024

近几年新型冠状病毒COVID-19的迅速传播,引起了全球对传染病防控和快速病毒检测技术的高度关注。表面增强拉曼光谱(SERS)作为一种光学分析技术,凭借其独特的分子指纹特性和高检测灵敏度的特点,成为生物医学检测领域的有力工具,对可能大规模暴发的流行性病毒灵敏迅速的检测以及监控提供新颖、高效的光学解决方案。本文对从2021年以来开展的DNA、RNA病毒,尤其是威胁人类生命健康的流行性病毒检测工作当中使用的标记、非标记SERS技术进行梳理,从SERS基底结构建构及功能化修饰,分子探针的设计,高速响应、高灵敏度检测模型构建,生物技术、机器学习方法的联合使用等方面,特别是基于便携式、手持式拉曼光谱仪的研究,对SERS技术在病毒检测领域的应用进展进行了总结和展望。

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Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus

Small Science, Год журнала: 2024, Номер 4(11)

Опубликована: Авг. 10, 2024

Lateral flow immunoassays (LFIAs) are easy-to-use antigen tests that provide different signal readouts, with colorimetric readouts being the most commonly used. However, these analytical devices have relatively low sensitivity and produce semiquantitative results, limiting their diagnostic applications. Herein, we address challenges by implementing a digital surface-enhanced Raman spectroscopy (SERS)-based LFIA for accurate ultrasensitive quantitative detection of SARS-CoV-2 nucleocapsid (N) protein. Compared average SERS intensity measurements, approach allowed to overcome fluctuations in scattering signals, thereby increasing assay. Our method exhibited quantification range viral protein nasal swabs from 0.001 10 pg mL-1, limit down 1.9 aM (0.9 fg mL-1), improving LFIAs conventional-SERS-based several orders magnitude. Importantly, this shows an 0.03 TCID50 which is greater than reported other immunoassays. In conclusion, successfully demonstrate robust SARS-CoV-2N at ultralow concentrations. The improvement may pave way translate technology into arena microbes disease biomarkers.

Язык: Английский

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Near-infrared responsive gold nanorods for highly sensitive colorimetric and photothermal lateral flow immuno-detection of SARS-CoV-2

Analytical Methods, Год журнала: 2024, Номер 16(16), С. 2597 - 2605

Опубликована: Янв. 1, 2024

We developed a colorimetric and photothermal dual-mode LFIA platform for sensitive selective SARS-CoV-2 N protein detection based on gold nanorods.

Язык: Английский

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Optical lateral flow assays in early diagnosis of SARS-CoV-2 infection

Analytical Sciences, Год журнала: 2024, Номер 40(9), С. 1571 - 1591

Опубликована: Май 17, 2024

So far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have advantages rapid detection, simple operation, low cost, ease mass production, no need for special devices professional operators, which make them suitable self-testing at home. This review focuses on SARS-CoV-2 based optical LFAs including colorimetric, fluorescent (FL), chemiluminescent (CL), surface-enhanced Raman scattering (SERS) detection antigens nucleic acids. types recognition components, modes used antigen labels employed in different LFAs, strategies improve sensitivity were reviewed. Meanwhile, coupled with acid amplification techniques CRISPR–Cas systems acids summarized. We hope this provides research mentalities developing highly sensitive that can be home infection.

Язык: Английский

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Establishment of a Raman nanosphere based immunochromatographic system for the combined detection of Influenza A and B viruses’ antigens on a single T-line

Nanotechnology, Год журнала: 2024, Номер 35(50), С. 505501 - 505501

Опубликована: Сен. 25, 2024

A simple and rapid system based on Raman nanosphere (R-Sphere) immunochromatography was developed in this study for the simultaneous detection of Influenza A, B virus antigens a single test line (T-line). Two types R-Sphere with different characteristic spectrum were used as signal source, which labeled monoclonal antibodies against FluA, FluB (tracer antibodies), respectively. mixture containing anti-FluA antibody anti-FluB (capture antibody) sprayed T-line goat anti-chicken IgY coated C-line, antigen solution known concentration detected by strip lateral flow surface-enhanced spectroscopy (SERS). The scanned spectrometer SERS signals collected. Simultaneous specific recognition FluA achieved analyzing signals. findings indicated that could identify qualitative manner just 15 minutes, minimum threshold 0.25 ng ml

Язык: Английский

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Development of an Electrochemical Sensor for SARS-CoV-2 Detection Based on Loop-Mediated Isothermal Amplification

Biosensors, Год журнала: 2023, Номер 13(10), С. 924 - 924

Опубликована: Окт. 11, 2023

The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) more than 6 million deaths all over world, demonstrating need for a simple, fast and cost-effective point-of-care (POC) test detection of virus. In this work, we developed an electrochemical sensor SARS-CoV-2 virus on clinical samples based loop-mediated isothermal amplification (LAMP). With development novel sensor, time each measurement is significantly reduced avoiding DNA extraction step replacing it with inactivation sample heating at 95 °C 10 min. To make reaction compatible pre-treatment, RNase inhibitor was added directly to premix. LAMP product measured in novel, easy-to-use manufactured containing custom-made screen-printed carbon electrode. Electrochemical performed portable potentiostat, methylene blue used as redox-transducing molecule. achieved limit 62 viral copies 100% specific performance validated nasopharyngeal samples, obtaining sensibility specificity compared gold standard RT-PCR method.

Язык: Английский

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A sensitive gold nanoflower-based lateral flow assay coupled with gold staining technique for the detection of SARS-CoV-2 antigen

Microchimica Acta, Год журнала: 2024, Номер 191(7)

Опубликована: Июнь 29, 2024

Язык: Английский

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Molecular Understanding of the Surface-Enhanced Raman Spectroscopy Salivary Fingerprint in People after Sars-COV-2 Infection and in Vaccinated Subjects

Chemosensors, Год журнала: 2024, Номер 12(7), С. 136 - 136

Опубликована: Июль 11, 2024

The rapid spread of SARS-COV-2 and the millions worldwide deaths hospitalizations have prompted an urgent need for development screening tests capable rapidly accurately detecting virus, even in asymptomatic people. easy collection biomarker content saliva, together with label-free informative power surface-enhanced Raman spectroscopy (SERS) analysis driven creation point-of-care platforms identifying people COVID-19. Indeed, different salivary fingerprints were observed between uninfected infected Hence, we performed a retrospective SERS spectra from samples COVID-19-infected -vaccinated subjects to understand if viral components and/or immune response are implicated spectral variations. high sensitivity proposed SERS-based method highlighted persistence molecular alterations saliva up one month after first positive swab, when subject tested negative antigenic test. Nevertheless, no specific patterns attributable some proteins immunoglobulins involved COVID-19 infection its progression found, differences peak intensity, presence, position fingerprint.

Язык: Английский

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