Oral supplementation of curcumin-encapsulated chitosan nanoconjugates as an innovative strategy for mitigating nickel-mediated hepatorenal toxicity in rats

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Abstract Nickel pollution adversely affects human health and causes various disorders, mainly hepatic renal dysfunction. The present work focused on a comparative evaluation of the pure form curcumin (CU) with curcumin-encapsulated chitosan nanoconjugates (CS/CU NCs), mitigation delirious effects Ni hepatorenal tissue. Forty-two male rats were allocated into 6 groups (n = 7 for each) as follows: (1) control, (2) CU, (3) CS/CU NCs, (4) Ni, (5) + (6) NCs. After 30 days, blood tissue (liver kidneys) collected to measure biomarkers, oxidant/antioxidant balance, inflammatory gene expression, liver kidney histopathology, immunohistochemistry. Results revealed disruption functions, oxidative stress, markers at biochemical molecular levels associated severe histopathological alterations abnormal immunohistochemical expression caspase-3 cyclooxygenase-2. On contrary, treatment Ni-intoxicated NCs markedly mitigated adverse effect via regulation inflammatory, apoptotic markers. study provides novel nanoformulation using CS NPs encapsulation that selectively targets injured cells improves beneficial CU enhancing antioxidant activity regulating both

Язык: Английский

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Epigallocatechin gallate-rich fraction alleviates histamine-induced neurotoxicity in rats via inactivating caspase-3/JNK signaling pathways

Food and Chemical Toxicology, Год журнала: 2024, Номер 193, С. 115021 - 115021

Опубликована: Сен. 23, 2024

Язык: Английский

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Pink1/Parkin signaling mediates pineal mitochondrial autophagy dysfunction and its biological role in a comorbid rat model of depression and insomnia

Brain Research Bulletin, Год журнала: 2024, Номер unknown, С. 111141 - 111141

Опубликована: Дек. 1, 2024

Using a chronic unpredictable mild stress (CUMS) combined with multi-platform water environment sleep deprivation (SD) as an animal model, the occurrence and development of human depression insomnia were simulated. The abnormal mitochondrial autophagy signaling caused by putative kinase 1/Parkin E3 ubiquitin protein ligase (Pink1/Parkin) pathway directly affects normal secretion melatonin pineal gland, which may explain pathogenesis insomnia. This study aims to explore depression-like behavior, changes, central oxidative response, damage, secretion, histopathological changes expression Pink1/Parkin signaling-related factors in CUMS+SD rats. results showed that levels reactive oxygen species (ROS) cerebrospinal fluid rats significantly increased along inflammatory Interleukin-1β (IL-1β) nuclear factor kappa-B (NF-κB) fluid. In addition, number gland cells decreased, cell boundaries became blurred, volume shrank, apoptotic bodies appeared tissue under HE staining, indicating inflammation. Sleep further disrupted damage factors, including histamine (MDA), glutathione (GSH), catalase (CAT), CUMS + SD Transmission electron microscopy revealed autophagy. 5-hydroxytryptamine (5-HT) aromatic amine-N-acetyltransferase (AANAT) fluid, well decreased. Additionally, IL-1β, NF-κB, Pink1, Parkin increased. microtubule-associated 1 light chain 3-β (LC3), selective adaptor (P62), cytochrome c oxidase IV (COXIV), outer membrane translocation enzyme 20 (TOM20) proteins downstream was enhanced, while brain-derived neurotrophic (BDNF), Beclin 1, BCL2 interacting 3 (BNIP3) negatively regulated. specifically respond affecting synthesis gland. summary, rats, is closely related specific impairment pathway, mediate

Язык: Английский

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Betaine alleviates methomyl-triggered oxidative stress-mediated cardiopulmonary inflammation in rats through iNOS/Cox2 and Nrf2/HO1/Keap1 signaling pathway

Toxicology and Applied Pharmacology, Год журнала: 2024, Номер 495, С. 117223 - 117223

Опубликована: Дек. 31, 2024

Язык: Английский

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