Life Sciences, Год журнала: 2024, Номер 358, С. 123104 - 123104
Опубликована: Окт. 2, 2024
Язык: Английский
Опубликована: Янв. 1, 2025
Ethnopharmacological relevance: Sini Decoction (SND) is a traditional classic formula from Treatise on Fevers. SND has been proven to be an effective herbal decoction for the treatment of cardiovascular diseases. Current studies have shown that potential treat myocardial ischemiaAim study: Based network pharmacology, in vitro and vivo experiments, this study investigated therapeutic effects ischemia possible pharmacological mechanisms.Materials methods: Firstly, material basis admission components were detected by ultra - performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS), then pharmacology analysis was performed according experimental results. Myocardial target-related retrieved GeneCards database Traditional Chinese Medicine (TCM) System Pharmacology Database Analysis Platform (TCMSP) respectively. Then, protein interaction (PPI) constructed screening key targets. Moreover, gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses employed predict biological function mechanism anti-myocardial effect SND. In vitro, CCK-8 assay, determination cytokines (LDH, CK, CK MB, GSH, CAT, MDA, SOD), cell death rate laser confocal microscopy, apoptosis reactive oxygen species (ROS) flow cytometry utilized verify anti oxidation proliferation H9c2 cells. For rats PI3K/AKT MAPK signaling pathways Western blot experiments. Hematoxylin-eosin staining used observe pathological changes heart tissue, TUNEL assay detect cardiac cells.Results: Serum pharmacochemistry identified 17 active (13 prototype 4 metabolic components) serum containing Decoction. may play role through pathways. Through animal experiments can activate pathway, inhibit reduce oxidative stress, improve apoptosis, thereby achieving ischemia.Conclusions: The results indicate treats MI activating inhibiting pathway.
Язык: Английский
Процитировано
0
Journal of Cellular Neuroscience and Oxidative Stress, Год журнала: 2025, Номер 17(1), С. 1237 - 1244
Опубликована: Фев. 27, 2025
Chemotherapy drugs such as carboplatin are commonly used to treat various cancers, including testicular, lung, and ovarian cancer. Although primarily targets cancer cells, it can also damage healthy neurons, leading potential adverse effects. Notably, some side effects of therapy associated with nerve cells the nervous system. The aim our study was investigate protective chrysin (Chr) against carboplatin-induced toxicity in SH-SY5Y neuronal cells. In this study, ameliorative Chr on cellular were evaluated through cell viability assays, lipid peroxidation (LPO) analysis assess antioxidant capacity, TUNEL assay, immunohistochemistry (IHC) staining, western blotting examine anti-apoptotic activities. results indicated that mitigates by reducing LPO levels expression cytochrome c (Cyt c) Bax, while increasing protein Bcl-2. demonstrated caused apoptosis causing DNA strand breaks treatment alleviated these These findings suggest use antioxidants, particularly Chr, may diminish apoptotic could provide insights into therapeutic strategies for mitigating carboplatin.
Язык: Английский
Процитировано
0
Life, Год журнала: 2025, Номер 15(3), С. 459 - 459
Опубликована: Март 14, 2025
Vincristine (VCR) is a chemotherapeutic agent classified as vinca alkaloid. Royal jelly (RJ) significant bee product produced by worker bees, characterized its high protein content. This study aims to investigate the protective effects of RJ against VCR-induced liver damage. VCR was intraperitoneally administered at dose 0.1 mg/kg body weight (b.w.) and orally doses 150 300 b.w. Both treatments were applied rats on days 1–6 9–14. The composition analyzed using LC-MS/MS, revealing presence 15 different phytochemical compounds with strong antioxidant properties. Serum samples obtained from for ALT, ALP, AST levels. While these enzyme levels significantly elevated in group, notable reduction observed following administration. Additionally, SOD, CAT, GPx, GSH parameters, along MDA levels, evaluated tissue samples. results indicated decrease activities/levels an increase group. Furthermore, ELISA used assess JAK2, STAT3, mTOR/PI3K/AKT signaling pathways. administration led JAK2 STAT3 In addition, mRNA transcription inflammation (NF-κB, TNF-α, IL-1β), endoplasmic reticulum (ER) stress (IRE-1, GRP78, PERK, ATF-6), autophagy markers (LC3A LC3B) examined. A inflammation, ER stress, autophagy-related VCR-treated Lastly, expression Bax, Bcl-2, Caspase-3, NF-κB evaluated. treatment increased Caspase 3, whereas Bcl-2 decreased. However, administration, all parameters reversed, demonstrating improvements. conclusion, findings suggest that may exert effect
Язык: Английский
Процитировано
0
Nutrients, Год журнала: 2024, Номер 16(21), С. 3768 - 3768
Опубликована: Ноя. 2, 2024
Objective: To investigate the effects of licorice functional ingredient intake on blood pressure, explore its potential mechanisms action, and provide safety information for personalized nutritional interventions in special populations application licorice-derived foods. Methods: PubMed, Cochrane Library, Medline, Embase, EBSCO, ScienceDirect, Web Science databases were searched from inception to 31 August 2024. Randomized controlled trials (RCTs) investigating or components included. The range continuous variables was assessed using weighted mean difference (WMD) with 95% confidence intervals. Genes associated hypertension screened an online database. Machine learning, receiver operating characteristic(ROC) curve analysis, molecular docking, gene set enrichment analysis (GSEA) employed underlying licorice-induced pressure fluctuations. Results: Eight RCTs (541 participants) included meta-analysis, which indicated containing glycyrrhizic acid (GA) as main component increased systolic (SBP) diastolic (DBP) (SBP: WMD [95% CI] = 3.48 [2.74, 4.21], p < 0.001; DBP: 1.27 [0.76, 1.78], 0.001). However, dominated by flavonoids(LF) had no significant effect SBP DBP 0.58 [−1.15, 2.31], 0.511; 0.17 [−1.53, 1.88], 0.843). Three machine learning algorithms identified five biomarkers hypertension: calmodulin 3 (CALM3), cluster differentiation 9 (CD9), growth factor independence 1B transcriptional repressor (GFI1B), myosin light chain kinase (MYLK), Ras suppressor-1 (RSU1). After removing lower validity reliability, GFI1B, MYLK, RSU1 selected subsequent analysis. network toxicology results suggested that GA metabolite glycyrrhetinic may act RSU1, influencing fluctuations modulating nitrogen metabolism signaling pathways. Conclusions: There distinct differences pressure. Functional constituents shown increase both DBP, whereas those LF did not exhibit hypertensive mechanism involve modulation regulate metabolic
Язык: Английский
Процитировано
2
ACS Omega, Год журнала: 2024, Номер 9(49), С. 48821 - 48834
Опубликована: Ноя. 28, 2024
Glycyrrhetin urea has emerged as a privileged scaffold with anti-inflammatory activity for the treatment and prevention of acute kidney injury (AKI). In this study, structural modifications A ring glycyrrhetinic acid yielded series derivatives, among which compound 7o exhibited most promising activity. was confirmed to interact STING through cellular heat shift assay inhibit STING/NF-κB pathway in RAW264.7 cells. It acted on pathway, inhibited NF-κB phosphorylation, subsequently reduced level release inflammatory factors. Additionally, significantly increased survival rate renal tubular epithelial cells, demonstrating protective effect against cisplatin-induced cell death mitigating inflammation activation. The vivo AKI mouse model showed that downregulated serum creatinine (Scr), blood nitrogen (BUN), levels factors (IL-1β, IL-6, TNF-α), thereby improving function. Morphological analysis revealed attenuated injury. Therefore, represents lead AKI.
Язык: Английский
Процитировано
2
Life Sciences, Год журнала: 2024, Номер 358, С. 123104 - 123104
Опубликована: Окт. 2, 2024
Язык: Английский
Процитировано
1