Biomarkers for sepsis: more than just fever and leukocytosis—a narrative review

Critical Care, Год журнала: 2022, Номер 26(1)

Опубликована: Янв. 6, 2022

Язык: Английский

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Sepsis Biomarkers: Advancements and Clinical Applications—A Narrative Review

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(16), С. 9010 - 9010

Опубликована: Авг. 19, 2024

Sepsis is now defined as a life-threatening syndrome of organ dysfunction triggered by dysregulated host response to infection, posing significant challenges in critical care. The main objective this review evaluate the potential emerging biomarkers for early diagnosis and accurate prognosis sepsis management, which are pivotal enhancing patient outcomes. Despite advances supportive care, traditional like C-reactive protein procalcitonin have limitations, recent studies identified novel with increased sensitivity specificity, including circular RNAs, HOXA distal transcript antisense RNA, microRNA-486-5p, C, triiodothyronine, prokineticin 2. These hold promising detection prognostication sepsis. They play crucial role not only but also guiding antibiotic therapy evaluating treatment effectiveness. introduction point-of-care testing technologies has brought about paradigm shift biomarker application, enabling swift real-time evaluation. these advancements, persist, notably concerning variability lack standardized thresholds. This summarizes latest advancements research, spotlighting progress clinical implications. It emphasizes significance multi-biomarker strategies feasibility personalized medicine management. Further verification on large scale their integration into practice advocated maximize efficacy future treatment.

Язык: Английский

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An Update on Sepsis Biomarkers

Infection and Chemotherapy, Год журнала: 2020, Номер 52(1), С. 1 - 1

Опубликована: Янв. 1, 2020

Sepsis is a dysregulated systemic reaction to common infection, that can cause lifethreatening organ dysfunction.Over the last decade, mortality rate of patients with sepsis has decreased as long are treated according recommendations Surviving Campaign, but still unacceptably high.Patients at risk should therefore be identified prior onset dysfunction and this requires rapid diagnosis prompt initiation treatment.Unfortunately, there no gold standard for traditional culture methods time-consuming.Recently, in order overcome these limitations, biomarkers which could help predicting prognosis sepsis, well being useful monitoring response treatments, have been identified.In addition, recent advances led development newly classes such microRNAs, long-non-coding RNAs, human microbiome.This review focuses on latest information used predict sepsis.

Язык: Английский

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Long Noncoding RNA: Regulatory Mechanisms and Therapeutic Potential in Sepsis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2021, Номер 11

Опубликована: Май 12, 2021

Sepsis is a life-threatening organ dysfunction caused by dysregulated host response to infection and characterized hyperinflammatory state accompanied immunosuppression. Long noncoding RNAs (lncRNAs) are longer than 200 nucleotides have important roles in mediating various biological processes. Recently, lncRNAs were found exert both promotive inhibitory immune functions sepsis, thus participating sepsis regulation. Additionally, several studies revealed that involved sepsis-induced dysfunctions, including cardiovascular dysfunction, acute lung injury, kidney injury. Considering the lack of effective biomarkers for early identification specific treatment may be promising even targets therapies. This review systematically highlights recent advances regarding sheds light on their use as potential sepsis.

Язык: Английский

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Long Non-coding RNA MEG3 Promotes Renal Tubular Epithelial Cell Pyroptosis by Regulating the miR-18a-3p/GSDMD Pathway in Lipopolysaccharide-Induced Acute Kidney Injury

Frontiers in Physiology, Год журнала: 2021, Номер 12

Опубликована: Апрель 29, 2021

Background and Objective: Acute kidney injury (AKI) is a complication of sepsis. Pyroptosis gasdermin D (GSDMD)-mediated tubular epithelial cells (TECs) play important roles in pathogenesis sepsis-associated AKI. Long non-coding RNA (lncRNA) maternally expressed gene 3 ( MEG3 ), an imprinted involved tumorigenesis, implicated pyroptosis occurring multiple organs. Herein, we investigated the role mechanisms regulation TEC lipopolysaccharide (LPS)-induced Materials Methods: Male C57BL/6 mice primary human TECs were treated with LPS for 24 h to establish animal cell models, respectively, sepsis-induced Renal function was assessed by evaluation serum creatinine urea levels. tubule score Periodic acid-Schiff staining. evaluating expression caspase-1, GSDMD, inflammatory factors IL-1β IL-18. Cellular analyzing release rate LDH, IL-1β, IL-18, using EtBr EthD2 renal tissues detected RT-qPCR. The molecular LPS-induced AKI through bioinformatics analysis, RNA-binding protein immunoprecipitation, dual luciferase reporter assays, rescue experiment. Results: both these models significantly up-regulated. -knockdown showed decreased number pyroptotic cells, down-regulated secretion compared those controls; however, there no difference caspase-1 between knockdown controls. Bioinformatics analysis screened out miR-18a-3P, further experiments demonstrated that controls GSDMD acting as ceRNA miR-18a-3P promote pyroptosis. Conclusion: Our study demonstrates lncRNA promoted regulating miR-18a-3p/GSDMD pathway

Язык: Английский

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Regulatory Role of Non-Coding RNAs on Immune Responses During Sepsis

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Дек. 9, 2021

Sepsis is resulted from a systemic inflammatory response to bacterial, viral, or fungal agents. The induced by these microorganisms can lead multiple organ system failure with devastating consequences. Recent studies have shown altered expressions of several non-coding RNAs such as long (lncRNAs), microRNAs (miRNAs) and circular (circRNAs) during sepsis. These transcripts also been found participate in the pathogenesis through different mechanisms. NEAT1, MALAT1, THRIL, XIST, MIAT TUG1 are among lncRNAs that pathoetiology sepsis-related complications. miR-21, miR-155, miR-15a-5p, miR-494-3p, miR-218, miR-122, miR-208a-5p, miR-328 miR-218 examples miRNAs participating Finally, tens circRNAs circC3P1, hsa_circRNA_104484, hsa_circRNA_104670 circVMA21 circ-PRKCI affect In current review, we describe role three classes noncoding

Язык: Английский

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Long Non-Coding RNAs as Biomarkers and Therapeutic Targets in Sepsis

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Сен. 22, 2021

Sepsis, an infection-induced systemic inflammatory disorder, is often accompanied by multiple organ dysfunction syndromes with high incidence and mortality rates, those who survive are left long-term sequelae, bringing great burden to social economy. Therefore, novel approaches solve this puzzle urgently needed. Previous studies revealed that long non-coding RNAs (lncRNAs) have exerted significant influences on the process of sepsis. The aim review summarize our understanding lncRNAs as potential sepsis-related diagnostic markers therapeutic targets, provide new insights into diagnosis treatment for In study, we also introduced current sepsis discussed their limitations, while research advances in promising biomarkers prognosis Furthermore, roles sepsis-induced were illustrated terms different systems. Nevertheless, further should be carried out elucidate underlying molecular mechanisms pathological

Язык: Английский

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Early Diagnosis of Sepsis: The Role of Biomarkers and Rapid Microbiological Tests

Seminars in Respiratory and Critical Care Medicine, Год журнала: 2024, Номер 45(04), С. 479 - 490

Опубликована: Июль 1, 2024

Abstract Sepsis is a medical emergency resulting from dysregulated response to an infection, causing preventable deaths and high burden of morbidity. Protocolized accurate interventions in sepsis are time-critical. Therefore, earlier recognition cases allows for preventive interventions, early treatment, improved outcomes. Clinical diagnosis by clinical scores cannot be considered diagnosis, given that underlying molecular pathophysiological mechanisms have been activated the preceding hour or days. There lack widely available tool enhancing preclinical sepsis. Sophisticated technologies prediction several limitations, including costs. Novel fast microbiological focusing on bedside point-of-care combined testing reach most settings where represents challenge.

Язык: Английский

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Navigating the Modern Landscape of Sepsis: Advances in Diagnosis and Treatment

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 7396 - 7396

Опубликована: Июль 5, 2024

Sepsis poses a significant threat to human health due its high morbidity and mortality rates worldwide. Traditional diagnostic methods for identifying sepsis or causative organisms are time-consuming contribute rate. Biomarkers have been developed overcome these limitations currently used diagnosis, prognosis prediction, treatment response assessment. Over the past few decades, more than 250 biomarkers identified, of which in clinical decision-making. Consistent with diagnosing sepsis, there is no specific sepsis. Currently, general conservative includes timely antibiotic use hemodynamic support. When planning sepsis-specific treatment, it important select most suitable patient, considering heterogeneous nature This comprehensive review summarizes current evolving therapeutic approaches

Язык: Английский

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LncRNA MEG3 suppresses hepatocellular carcinoma by stimulating macrophage M1 polarization and modulating immune system via inhibiting CSF-1 in vivo/vitro studies

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 281, С. 136459 - 136459

Опубликована: Окт. 11, 2024

Язык: Английский

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