Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment DOI Creative Commons
Nehal Mohamed,

Osama Fekry Ahmed Al Balah,

M. Refat

и другие.

The Journal of Basic and Applied Zoology, Год журнала: 2025, Номер 86(1)

Опубликована: Март 2, 2025

Abstract Background The combination of low level laser therapy (LLLT) and nano-sized particles (NPs) chitosan (Chit) is hypothesized to be more effective in skin wound healing. A total 63 CD1 mice were divided into the normal group injured groups that subjected a 21-day course exposure (650 ± 5 nm, power density 180 mw/cm 2 ), topically applied 2% Chit NPs both. Results On day 21, healing rate reached 100% treated by plus while other single treatments did not reach this record. After 48 h, with significantly enhanced re-epithelization, granulation, inflammation, angiogenesis scores compared non-treated group. 21 days, showed improvements some histological parameters, but combined treatment exhibited complete epidermal remodeling decrease inflammatory exudate. During late phase, count RBCs hemoglobin% was increased NPs. In contrast, differential neutrophils, lymphocytes, monocytes markedly lower than untreated Within received significant ( P < 0.001) elevated serum levels TNF-α IL-1β Conclusions Therapy or alone beneficial effects, both accelerated displayed highest improved lesion score.

Язык: Английский

Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment DOI Creative Commons
Nehal Mohamed,

Osama Fekry Ahmed Al Balah,

M. Refat

и другие.

The Journal of Basic and Applied Zoology, Год журнала: 2025, Номер 86(1)

Опубликована: Март 2, 2025

Abstract Background The combination of low level laser therapy (LLLT) and nano-sized particles (NPs) chitosan (Chit) is hypothesized to be more effective in skin wound healing. A total 63 CD1 mice were divided into the normal group injured groups that subjected a 21-day course exposure (650 ± 5 nm, power density 180 mw/cm 2 ), topically applied 2% Chit NPs both. Results On day 21, healing rate reached 100% treated by plus while other single treatments did not reach this record. After 48 h, with significantly enhanced re-epithelization, granulation, inflammation, angiogenesis scores compared non-treated group. 21 days, showed improvements some histological parameters, but combined treatment exhibited complete epidermal remodeling decrease inflammatory exudate. During late phase, count RBCs hemoglobin% was increased NPs. In contrast, differential neutrophils, lymphocytes, monocytes markedly lower than untreated Within received significant ( P < 0.001) elevated serum levels TNF-α IL-1β Conclusions Therapy or alone beneficial effects, both accelerated displayed highest improved lesion score.

Язык: Английский

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