Synthesis and characterization of microbial mediated cadmium oxide nanoparticles

Microscopy Research and Technique, Год журнала: 2020, Номер 83(12), С. 1574 - 1584

Опубликована: Авг. 5, 2020

Abstract Microbial mediated synthesis of metallic nanoparticles constitutes as effective and promising approach for the development antibacterial materials in field bioengineering biomedicine. We prepared Cadmium oxide nanoaprticles (CdO NPs) utilizing Penicillium oxalicum , cadmium acetate solution via coprecipitate method. The elemental composition morphology these synthesized CdO NPs were examined through X‐ray diffraction (XRD), UV–Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Energy dispersive (EDS). Furthermore, we evaluated bactericidal potential using Escherichia coli ( E.coli ), Staphylococcus aureus S.aureus Bacillus cereus B.cereus Pseudomonas aeruginosa P. ). Dimethyl sulfoxide was used negative control while erythromycin positive control. XRD spectrum revealed cubic crystalline with 22.94 nm size UV showed absorbance peak at 297 2.5 eV band gap energy. FTIR depicted O─H carboxylic groups along stretching vibration. EDS presence organic compounds on Cd O over surface. SEM results spherical shape NPs. exhibited highly potent activity against selected strains demonstrated less optical density 0.086 after 24 hr. Owing to significant NPs, broad application prospects extensive biomedical applications is indicated.

Язык: Английский

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Complete chloroplast genomes of eight Delphinium taxa (Ranunculaceae) endemic to Xinjiang, China: insights into genome structure, comparative analysis, and phylogenetic relationships

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Июнь 26, 2024

Abstract Background Delphinium L. represents a taxonomically intricate genus of significant phylogenetic and economic importance in Ranunculaceae. Despite the existence few chloroplast genome datasets, comprehensive understanding structures selective pressures within remains unknown. Furthermore, several taxa this are exclusively found Xinjiang, China, region renowned for its distribution diversity Chinese Central Asian species. Therefore, investigating features genomes area will provide valuable insights into evolutionary processes relationships genus. Results In study, eight newly completed examined, ranging length from 153,979 bp to 154,284 bp. Alongside these, analysing six previously reported re-annotated , 111 unique genes identified across all samples. Genome structure, distributions simple sequence repeats short dispersed repeats, as well gene content similar among these taxa. Nine hypervariable intergenic spacers protein coding regions, including ndh F- trn L (TAG) rpl 16-intron, 33, rps 15, 18, K (TTT) - Q (TTG) P (TGG) psa J, T (GGT) psb D ycf 1, 13 perennial . Selective pressure codon usage bias plastid performed 14 Phylogenetic analysis based on plastomes, alongside two Aconitum (Ranunculaceae) species serving outgroup taxa, reveals monophyletic nature Our findings further discern distinct clades: (clade I) annual II). addition, compared with nrDNA ITS topology, cytological data morphological characters, D. mollifolium maackianum showed potential involvement hybridization or polyploidization processes. Excluding species, exhibits stronger consistency morphology-based system that utilized seed morphology. Conclusion This study first plastomic variations examination complete plastomes. The structure is other angiosperms possesses typical quadripartite conserved arrangement features. variation non-coding regions larger than genome. Through DNA divergence plastomes subsequent phylogenomic analyses 1 promising molecular markers. These highly variable loci held future phylogeographic studies whole concatenation 132 spacer 77 protein-coding ITS, support monophyly clusters together one clade crucial systematic, research studies.

Язык: Английский

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Research Advances in the Biological Characteristics and Systematic Classification of <i>Delphinium</i>

Botanical Research, Год журнала: 2025, Номер 14(03), С. 103 - 110

Опубликована: Янв. 1, 2025

Язык: Английский

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Foliar micromorphology and its role in identification of the Apocynaceae taxa

Microscopy Research and Technique, Год журнала: 2020, Номер 83(7), С. 755 - 766

Опубликована: Март 2, 2020

Abstract In the present study, we evaluate importance of foliar epidermal micromorphological characteristics Apocyanaceae for accurate identification and classification. The species were collected from University Peshawar's main campus in spring season to observe its qualitative quantitative features. length width guard cells, stomatal pore subsidiary trichomes, crypts on both sides leaf examined. Many observed be hypostomatic. Plumeria rubra , Raulfia serpentine Thevetia peruviana Trachelospermum lucidum Alstonia scholaris Catharanthus roseus demonstrated hypostomatic leaves. Nearly all investigated had anisocytic type stomata only or combination with other types upper lower epidermis. Carissa carandas anomocytic, anisocytic, cyclocytic epidermis, epidermis showed variations type, such as stephanocytic, brachyparacytic, hemiparacytic. Nerium oleander no specific shape but which enclosed inside many trichomes. taxonomic key based types, index value, statistical analysis, along shows link between selected plants species, will provide a baseline future anatomical studies. This study highlights undocumented characteristics. this helpful delimitation family Apocynaceae.

Язык: Английский

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Identification of novel, non‐edible oil seeds via scanning electron microscopy as potential feedstock for green synthesis of biodiesel

Microscopy Research and Technique, Год журнала: 2021, Номер 85(2), С. 708 - 720

Опубликована: Сен. 23, 2021

In the present era, environmental glitches associated with extensive emission of greenhouse gases (GHG) and energy crises caused by exhausting fossil fuel reservoirs has diverted researcher's interest toward alternative renewable sources. Biodiesel is a renewable, biodegradable, sustainable to petro-diesel. synthesized from non-edible seed oils preferred due its cost effectiveness eco-friendly nature. Hence, our study focused on investigation identification micromorphological characters six novel, oil feedstock for biodiesel production via scanning electron microscopy (SEM). Results light seeds revealed distinct variation in size (15.8-1.8 mm length 9.4-1.1 width), shape (round Cuneiform), color (from black yellowish green). Non-edible content fall range 28-38% (wt/wt). Free fatty acid (FFA) varied 0.56 2.06 mg KOH/g. Multivariate analysis was performed investigate correlation between three significant variables yielding such as potential synthesis, content, FFA principal component analysis. Ultra morphological surfaces SEM exhibited distinctive surface sculpturing, cell arrangement, shape, periclinal wall margins, protuberances, anticlinal shape. Seed sculpturing reticulate, semitectate, wrinkled, rugose, papillate, perforate, striate. Periclinal arrangements confirmed glabrous, raised, depressed, elevated, smooth, pentagonal, entire, ripple margins. Whereas, walls pattern demonstrated angular, wavy, deep, dentate, irregular, puzzled, elongated, curved, depressed. Finally, it concluded obtained results that could be possible useful tool disclosing veiled seeds, which provides information researchers their correct, authentic identification, classification modern synthetic system.

Язык: Английский

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Development of quality control parameters for two Bhutanese medicinal plants (Aster flaccidus Bunge and Aster diplostephioides (DC.) Benth. ex C.B.Clarke) using traditional and modern pharmacognostical platforms

Heliyon, Год журнала: 2024, Номер 10(3), С. e24969 - e24969

Опубликована: Янв. 22, 2024

Bhutan's scholarly traditional medical system is called Bhutanese Sowa Rigpa medicine (BSM). It was integrated with the modern healthcare in 1967. Over 200 medicinal plants are used to produce more than 100 poly-ingredient formulations. Although BSM supported by well-documented principles, pharmacopoeias, diagnostic procedures, treatment regimens, and quality assurance systems, control parameters have become essential distinguish closely related species prevent contamination from exogenous impurities. This study aims establish reliable analytical methods for Aster flaccidus Bunge diplostephioides (DC.) Benth. ex C.B. Clarke as ingredients BMS Furthermore, their reported phytochemicals biological activities also discussed this study. Standard pharmacognostic techniques, including macroscopical microscopical examinations of crude drugs, were employed two species. The physicochemical limits determined per World Health Organization (WHO)-recommended guidelines described Thai herbal pharmacopoeia. A high-performance thin-layer liquid chromatography (HPTLC) develop a comparative chromatogram/phytochemical fingerprint extracts obtained literature review conducted record isolated activities. Two possess macro- microscopic features such colour, appearance, shape. Physicochemical analysis drugs HPTLC fingerprinting methanol yielded adequate data differentiate confirm before adding them From review, only A. found be studied its phytochemical constituents, whereby 11 pure compounds aerial parts roots. current revealed distinct species-specific distinguishing features, ecological adaptation, micromorphology, anatomy, values, chromatograms. These can authenticate identity adulterations, thereby improving safety

Язык: Английский

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Micromorphological study of some Salsola species (Amaranthaceae) in Iran and its systematic significance using scanning electron microscopy

Microscopy Research and Technique, Год журнала: 2024, Номер 87(11), С. 2603 - 2624

Опубликована: Июнь 24, 2024

Abstract Using scanning electron microscopy (SEM), six Salsola species from Iran were examined for their epidermis, seed, and fruit micromorphology. Among them S. brachiata section Heterotricha , S dendroides incanescens orientalis Caroxylon kali Kali turcomanica Physurus . Epidermal cells are divided into three types. There diamond, irregular, polygonal cells, as well straight undulated walls. Studied of have smooth or sculptured surfaces, there main types surface ornamentation. is a significant difference between these based on the type hair density fruit. Seed shape color little systematic significance. The seed epidermis composed polygonal, elongated diamond cells. Although irregular testa most common, size can provide additional information useful diagnostic characteristics at both specific infraspecific levels. For taxonomic separation, current study provides novel insights micromorphological Research Highlights This article reports halophyte shown models adaptation to extreme habitats. These plants placed among ecological communities xerophytes. Here, first time, microstructural analysis has been investigated. Additionally, it new plant species' response conditions, possible strategies level.

Язык: Английский

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Scanning electron microscopic identification of ten novel, non‐edible oil seeds for bioenergy production

Microscopy Research and Technique, Год журнала: 2022, Номер 85(9), С. 3245 - 3255

Опубликована: Июнь 28, 2022

Abstract Biodiesel is a sustainable, inexpensive, and alternative energy source produced from vegetable oils animal fats. Precise authentic identification of oil yielding plant species very crucial. Therefore, scanning electron microscopy (SEM) was employed in our current investigation to study micromorphological characteristics ten novel seeds for their reliable identification. Macromorphological characters sample were explored by light microscopy. Seed size varied 16 6.2 mm length 18.4–4.5 width. shape ovoid cordial color beige brown. content ranged 25% 45% (w/w). Whereas free fatty acid (FFA) seed 0.42 3.4 mg KOH/g. potential Chamaerops humilis found be highest (98%) all. Besides, ultra‐structural observation demonstrated variation surface sculpturing which rugous, reticulate, perforate, striate, webby. Periclinal wall arrangements rough, ridged, depressed, thick curved whereas, anticlinal walls pattern showed wavy, smooth, raised, deep depressed. It ultimately concluded that Scanning could serve as an advanced tool representing hidden seeds. offers significant knowledge researchers local community accurate genuine Research Highlights Non‐edible promising bioenergy. Variation Micromorphological among selected Classification non‐edible plants via characters.

Язык: Английский

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Pollen diversity and its implications to the systematics of mimosaceous species by LM and SEM

Microscopy Research and Technique, Год журнала: 2020, Номер 84(1), С. 42 - 55

Опубликована: Авг. 27, 2020

Abstract In this study we have discussed pollen morphology of 14 mimosaceous species belonging to five genera by using light microscopy (LM) and scanning electron (SEM). The palynological features were determined both qualitative quantitative characters. characters include shape, colpi arrangement, exine sculpturing. Oblate spheroidal shaped pollens noted in Albizia procera , lebbeck Acacia tortilis ampliceps modesta subprolate Leucaena leucocephala Prosopis julifera nilotica prolate farnesiana glandulosa while spheroidal, sub oblate grains observed catechu Mimosa himalayana cineraria respectively. Sparsely foveolate, reticulate, scabrate, scrobiclate sculpturing observed. Colpi with sunken ornamentation seen P. juliflora L. absent rest the studied species. statistically SPSS software. current gives important morpho‐palynological for identifying validation close related similar taxa which will aid phylogenetic analysis Mimosaceae family.

Язык: Английский

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Implication of scanning electron microscopy as a tool for identification of novel, nonedible oil seeds for biodiesel production

Microscopy Research and Technique, Год журнала: 2021, Номер 85(5), С. 1671 - 1684

Опубликована: Дек. 16, 2021

Biodiesel is a promising, bio-based, renewable, nontoxic, environment friendly, and alternative fuel for petroleum derived fuels which helps to reduce dependency on conventional fossil fuels. In this study, six novel, nonedible seed oil producing feedstock were explored their potential sustainable production of biodiesel. It very important correctly identify yielding plant species. Scanning electron microscopy (SEM) was used as reliable tool authentic identification seeds. Macromorphological characters seeds studied with light (LM). Outcomes LM exposed distinctive variation in size from 16.3 3.2 mm length 12.4 0.9 width, shape varied oval triangular, color black brown. Oil content ranged 25 30% (w/w). Free fatty acid 0.32 2.5 mg KOH/g. Moreover, ultra structural study via SEM showed surface sculpturing, cell arrangement, shape, periclinal wall margins, protuberances, anticlinal shape. Surface sculpturing rugged, reticulate, varrucose, papillate, striate. Periclinal arrangements confirmed rough, wavy, raised, depressed, smooth, elevated whereas, walls pattern profuse undulating, grooved, deep, curved, depressed. concluded that could be latent advanced technique unveiling hidden micromorphological delivers valuable information researchers indigenous people precise recognition.

Язык: Английский

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