Jurnal Ilmiah Teknik Elektro Komputer dan Informatika,
Год журнала:
2022,
Номер
8(3), С. 342 - 342
Опубликована: Окт. 13, 2022
Scanning
electron
microscope
(SEM)
images
are
highly
valuable
in
different
scientific
applications
because
they
can
depict
extremely
small
entities.
SEM
sometimes
obtained
blurry,
that
such
an
issue
reduces
the
clarity
and
hampers
detection
of
important
features
image.
One
way
processing
unwanted
blurring
effect
is
to
use
image
sharpening,
which
aims
at
emphasizing
edges
so
output
appears
more
significant
observer
with
better-highlighted
details.
Many
sharpening
methods
exist,
but
not
all
efficient
as
may
introduce
artifacts,
unnatural
appearance,
contrast/brightness
modifications,
or
be
complicated
require
a
high
computational
cost.
algorithm
interest
generalized
unsharp
mask
(GUSM),
avoids
generation
artifacts
many
own
have
somewhat
simple
structure.
Still,
when
GUSM
applied
images,
it
provides
sharpness
modifies
contrast
brightness
well.
This
undesirable
proper
required
for
information.
Hence,
adapted
introduced
this
article
natural
without
modifying
filtered
images.
The
main
contribution
study
remove
enhancement
procedure
replace
smoothing
process
deliver
sharpness.
developed
AGUSM
verified
real-unclear
its
performance
appraised
against
methods,
outcomes
comparisons
evaluated
by
utilizing
advanced
metrics.
For
performed
experiments,
provided
satisfying
performances
appear
acutance
look
compared
original
counterparts
comparison
methods.
Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Июнь 24, 2022
Members
of
Polygonatum
are
perennial
herbs
that
have
been
widely
used
in
traditional
Chinese
medicine
to
invigorate
Qi,
moisten
the
lung,
and
benefit
kidney
spleen
among
patients.
However,
phylogenetic
relationships
intrageneric
taxonomy
within
long
controversial
because
complexity
their
morphological
variations
lack
high-resolution
molecular
markers.
The
chloroplast
(cp)
genome
is
an
optimal
model
for
deciphering
related
families.
In
present
study,
complete
cp
26
species
Trib.
Polygonateae
were
de
novo
assembled
characterized;
all
exhibited
a
conserved
quadripartite
structure,
is,
two
inverted
repeats
(IR)
containing
most
ribosomal
RNA
genes,
unique
regions,
large
single
sequence
(LSC)
small
(SSC).
A
total
8
highly
variable
regions
(rps16-trnQ-UUG,
trnS-GCU-trnG-UCC,
rpl32-trnL-UAG,
matK-rps16,
petA-psbJ,
trnT-UGU-trnL-UAA,
accD-psaI,
trnC-GCA-petN)
might
be
useful
as
potential
markers
identifying
identified.
clock
analysis
results
showed
divergence
time
occur
at
∼14.71
Ma,
verticillate
leaf
ancestral
state
this
genus.
Moreover,
based
on
88
genomes
strongly
supported
monophyly
Polygonatum.
also
suggested
Heteropolygonatum
may
sister
group
Polygonatum,
but
Disporopsis,
Maianthemum,
Disporum
diverged
earlier.
This
study
provides
valuable
information
further
identification,
evolution,
research
Microscopy Research and Technique,
Год журнала:
2022,
Номер
85(6), С. 2045 - 2060
Опубликована: Янв. 29, 2022
The
family
Euphorbiaceae
is
one
of
the
most
taxonomically
complex
and
diverse
among
angiosperm.
Therefore,
this
study
aims
to
document
pollen
morphology
taxa
collected
from
different
geographical
regions
Hainan
Island
South
China,
strengthen
taxonomy
support
subfamilial
classification.
includes
23
species
10
genera
bi-ovulate
subfamily
Phyllanthoideae
tri-ovulate
Crotonoideae,
Euphorbioideae,
Acalyphoideae
were
examined
under
light
scanning
electron
microscopy.
principal
component
analysis
was
performed
by
using
six
variables
show
variation
relationships
between
species.
A
significant
found
in
(exine
colpus
sculpturing).
croton-type
pattern
diagnostic
trait
Crotonoideae.
Euhphorboideae
mostly
homogenous
however,
muri
pattern.
Acalyphoideae,
have
fossulate
scabrate
verrucate
exine
sculpturing
that
can
delimit
rest
sub-families.
Phyllanthoideae,
showed
reticulate
exine,
tri
tetra-colporate
having
various
morphology.
maximum
size
Croton
hirtus
(59.49
×
60.86
μm)
minimum
Acalypha
indica
(10.86
73
μm).
Similarly,
width
observed
Euphorbia
pulcherrima
(8.3
Hence
traits
relevance
phylogenetic
define
boundaries
at
taxonomic
levels.
This
subfamilies
fill
palynological
gap
these
will
contribute
future
systematic
studies
Euphorbiaceae.Some
selected
four
including
(Euphorbiaceae)
Island.
both
qualitative
features
surface
membrane
morphology)
quantitative
(polar
axis,
equatorial
diameter,
length
width,
polar
area
clava
diameter.
Based
on
traits,
our
suggested
eurypalynous
condition
Euphorbiaceae.
Pollen
a
potential
be
used
as
an
additional
tool
ranks
classification
Agronomy,
Год журнала:
2022,
Номер
12(5), С. 1122 - 1122
Опубликована: Май 6, 2022
Mimosoideae
is
one
of
the
taxonomically
complex
subfamilies
Fabaceae.
Several
studies
have
reported
pollen
morphology
taxa
and
their
taxonomic
relevance,
but
no
such
study
was
found
specifically
for
Hainan
Island
in
southern
China.
Therefore,
present
designed
to
investigate
selected
explore
new
palynological
traits
support
strengthen
systematics
using
multiple
microscopic
techniques.
The
polar
axis,
equatorial
diameter
grains,
colpus
length
width
were
measured.
smallest
grain
size
Mimosa
pudica
(7.8
×
7.75
µm),
while
largest
Albizia
lebback
(87.54
77.97
µm).
Similarly,
significant
variation
exine
surface
patterns.
subfamily
considered
eurypalynous
because
traits.
In
addition,
also
quantitative
Comparatively,
features
be
helpful
at
genus
species
levels,
as
well
correct
identification
discrimination
taxa.
Hence,
this
gives
a
detailed
account
morphologies
certain
collected
from
different
geographical
regions
on
Island.
morphological
proven
potential
can
used
additional
tools
These
results
will
provide
basis
further
systematic
studies.
Microscopy Research and Technique,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 16, 2025
ABSTRACT
The
Papilionoideae
is
a
taxonomically
complex
subfamily
within
the
Fabaceae.
Pollen
characteristics
are
often
used
as
additional
diagnostic
features
in
taxonomy
of
Papilionoideae.
Unfortunately,
pollen
morphology
India
remains
poorly
understood.
This
study
aims
to
bridge
palynological
knowledge
gap
taxa,
focusing
on
Northeast
India.
In
this
investigation,
light
and
scanning
electron
microscopy
(SEM)
were
employed
examine
10
species
belonging
nine
genera
Papilionoideae:
Aeschynomene
americana
,
Cajanus
scarabaeoides
Calopogonium
mucunoides
Crotalaria
calycina
spectabilis
Grona
triflora
Lablab
purpureus
Pueraria
phaseoloides
Tephrosia
candida
Uraria
crinita
.
grains
prepared
using
Wodehouse's
method;
photomicrographs
taken
describe
measure
traits.
traits
such
size,
aperture
number,
shape
stable
traits,
while
exine
sculptures,
P/E
ratio,
between
two
adjacent
colpi
(GBC)
can
differentiate
taxa.
Most
(nine
species)
had
tricolporate
except
with
syncolporate
pollen.
Medium‐sized
was
observed
most
studied
(eight
species),
small‐sized
(
A.
C.
).
Three
distinct
shapes
identified
among
species:
oblate‐spheroidal
(six
prolate‐spheroidal
(three
prolate
Different
types
sculpture
reticulate,
fossulate,
fossulate‐verrucate,
perforate,
foveolate‐reticulate,
foveolate‐granulate,
rugulate‐perforate,
psilate‐perforate.
first
principal
component
analysis
(PCA)
axis
showed
60.3%
total
data
variance.
Polar
(PA),
equatorial
diameter
(ED),
apocolpium,
mesocolpium,
GBC,
breadth
(ColpiB),
P/E,
nexine
(>
0.7)
contribute
significantly
PCA.
Further,
taxonomic
key
has
been
based
morphological
characters
for
correct
identification.
strengthens
validates
subfamily's
status
will
serve
foundation
more
phylogenetic
molecular
research
Microscopy Research and Technique,
Год журнала:
2021,
Номер
85(4), С. 1410 - 1420
Опубликована: Ноя. 30, 2021
Pollen
micromorphology
is
not
only
used
to
check
the
functional
and
structural
evolution
in
plants
but
also
solve
taxonomic
problem
related
classification
of
plants.
Therefore,
keeping
view
significance
pollen
traits,
selected
taxa
subfamily
Caesalpiniaceae
was
collected
from
different
geographical
regions
Pakistan.
The
species
were
then
analyzed
under
both
light
microscopy
scanning
electron
techniques
investigate
importance
micromorphological
characters
identification
species.
Great
variation
recorded
equatorial
shape,
surface
ornamentation,
tectum,
polar
diameter,
exine
thickness.
However,
little
observed
type,
fertility
pollen.
shape
five
types
observed:
prolate,
prolate-spheroidal,
spheroidal-subprolate,
subspheroidal-prolate,
subspheroidal.
Four
psilate,
granulate,
clavate,
perforate,
recorded.
Tectum
types,
intactate,
reticulate
regulate,
medium
reticulate,
tactate,
striate,
observed.
Sexine
thicker
than
nexine
all
studied
largest
diameter
Caesalpinia
pulcherrima
64.1
μm
while
smallest
Parkinsonia
aculeata
26.1
μm.
found
C.
70.25
whereas
P.
27.57
All
pollens
tricolporate.
have
a
ratio
more
90%.
A
key
developed
show
features
delimit
for
correct
identification.
In
conclusion,
traits
useful
define
boundaries
at
various
ranks
will
strengthen
taxonomy
this
subfamily.
Besides,
study
explored
palynological
their
implication
Caesalpiniaceae.
Microscopy Research and Technique,
Год журнала:
2022,
Номер
85(6), С. 2026 - 2044
Опубликована: Янв. 24, 2022
Herbal
medicines
are
gaining
popularity
worldwide
for
human
healthcare
because
of
their
therapeutic
potential.
However,
adulteration
and
use
unauthentic
raw
herbals
as
substitutes
have
become
a
major
issue
the
local
communities
industry
reasons
safety
efficacy.
Therefore,
authentication
medicinal
plants
before
in
herbal
is
need
time.
Hence,
present
study
was
designed
with
an
aim,
to
authenticate
Lamiaceous
taxa
by
using
pollen
traits
observed
under
scanning
electron
microscopy.
Pollen
micro-morphological
studies
solve
problem
through
discrimination
correct
identification
Lamiaceae
species
from
adulterants.
Based
on
features,
were
further
divided
into
two
sub-families
Lamioideae
(tricolpate)
Nepetoideae
(hexa-colpate).
The
grains
found
small
medium-sized,
tricolpate,
radially/bilateral
symmetrical,
sub-spheroidal
oblate
shape.
Besides,
exine
patterns
bireticulate,
reticulate
micro-reticulate,
colpus
surface
sculpturing
psilate,
gemmate,
scabrate,
verrucate
also
been
reported.
A
significant
variation
pattern
reticulum,
thickness,
number
secondary
lumina
per
primary
lumen.
Similarly,
has
hexa
zono-colpate
but
tri
tetra
observed.
this
contributes
medicinally
important
microscopic
techniques
can
help
problem.
Highlights
Authentication
Lamiceous
carried
out
techniques.
Chemotaxonomic
characterization
used
accurate
taxa.
medicinal,
palynological
phytochemical
significance
evaluated.
seen
that
determination
species.
chemotaxonomic
characterization,
our
reason
