Novel Insights Into Telocyte Presence and Distribution in the Cornea of the Laughing Dove (Spilopelia senegalensis) Using Immunofluorescence Analysis

Microscopy Research and Technique, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

ABSTRACT The eye of the laughing dove ( Spilopelia senegalensis ) is uniquely adapted to thrive in arid environments, withstanding airborne particles and harsh conditions, demonstrating its remarkable structural functional resilience. This study aimed explore cellular composition dove's cornea, focusing on telocytes—a cell type previously unreported avian corneas. work represents first demonstration telocytes cornea any bird species. We used hematoxylin eosin (H&E) staining comprehensively visualize corneal layers, while silver staining, methylene blue, toluidine blue immunofluorescence with telocyte‐specific markers (CD34, PDGFRα, vimentin) were utilized identify confirm their distribution. was shown consist five unique layers: epithelium, Bowman's layer, stroma, Descemet's membrane, endothelium. Telocytes detected using silver, stains as spindle‐shaped cells branched extensions known telopodes. Double for PDGFRα CD34 confirmed existence across all layers. In intraepithelial sparsely distributed, possibly contributing epithelial maintenance signaling. identified, likely supporting integrity intercellular interactions. stroma exhibited highest abundance telocytes, highlighting critical role extracellular matrix remodeling connectivity. contribute stability this basement endothelium, they appear play roles endothelial repair, fluid regulation, signaling processes. vimentin revealed distinct localization patterns. While identified expression confined predominantly superficial region. Vimentin specifically localized telopodes, more evenly strong both central bodies partial co‐expression suggests specialization within telopode structure long‐range Our findings underscore function, particularly contributions adaptation environmental conditions. not only identifies time but also highlights presence suggesting broader maintaining homeostasis

Язык: Английский

Novel identification of telocytes in the skin of the desert tortoise (Testudo graeca): A correlative study using semithin section, transmission electron microscopy, and immunofluorescence

Research in Veterinary Science, Год журнала: 2025, Номер 191, С. 105689 - 105689

Опубликована: Май 6, 2025

Язык: Английский