Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 17, 2024
Abstract
Glycosylation
is
the
most
common
post‐translational
modification
of
proteins
and
regulates
a
myriad
fundamental
biological
processes
under
normal,
pathological
conditions.
Altered
protein
glycosylation
linked
to
malignant
transformation,
showing
distinct
glycopatterns
that
are
associated
with
cancer
initiation
progression
by
regulating
tumor
proliferation,
invasion,
metastasis,
therapeutic
resistance.
The
small
extracellular
vesicles
(sEVs)
released
cells
promising
candidates
for
monitoring
since
they
exhibit
similar
their
cell‐of‐origin.
However,
clinical
application
sEV
glycans
challenging
due
limitations
current
analytical
technologies
in
tracking
trace
amounts
sEVs
specifically
derived
from
tumors
circulation.
Herein,
GLYcan
PHenotype
(EV‐GLYPH)
assay
utilizes
microfluidic
platform
integrated
surface‐enhanced
Raman
scattering
multiplex
profiling
non‐small
cell
lung
clinically
validated.
For
first
time,
EV‐GLYPH
effectively
identifies
glycan
signatures
between
non‐transformed
malignantly
transformed
cells.
In
study
evaluated
on
40
patients,
successfully
differentiates
patients
early‐stage
nodules
benign
nodules.
These
results
reveal
potential
profile
noninvasive
diagnostics
prognostics,
opening
up
avenues
applications
understanding
role
cancer.
Journal of Extracellular Vesicles,
Год журнала:
2022,
Номер
11(9)
Опубликована: Сен. 1, 2022
Small
extracellular
vesicles
(sEVs)
provide
major
promise
for
advances
in
cancer
diagnostics,
prognostics,
and
therapeutics,
ascribed
to
their
distinctive
cargo
reflective
of
pathophysiological
status,
active
involvement
intercellular
communication,
as
well
ubiquity
stability
bodily
fluids.
As
a
result,
the
field
sEV
research
has
expanded
exponentially.
Nevertheless,
there
is
lack
standardisation
methods
isolation
from
cells
grown
serum-containing
media.
The
majority
researchers
use
media
harvest
employ
ultracentrifugation
primary
method.
Ultracentrifugation
inefficient
it
devoid
capacity
isolate
high
yields
without
contamination
non-sEV
materials
or
disruption
integrity.
We
comprehensively
evaluated
protocol
using
tangential
flow
filtration
size
exclusion
chromatography
sEVs
variety
human
murine
cell
lines,
including
HeLa,
MDA-MB-231,
EO771
B16F10.
directly
compared
performance
traditional
methods,
that
had
undergone
further
purification
by
chromatography,
separate
sEVs,
rigorously
characterised
properties
multiple
quantification
devices,
protein
analyses
both
image
nano-flow
cytometry.
enrich
consistent
populations,
with
similar
distributions
particles
ranging
up
200
nm.
However,
exceeds
isolating
significantly
higher
making
more
suitable
large-scale
applications.
Our
results
demonstrate
reliable
robust
approach
surpasses
yield,
reproducibility,
time,
costs
scalability.
These
advantages
allow
implementation
comprehensive
applications
downstream
investigations.
Bioactive Materials,
Год журнала:
2024,
Номер
38, С. 1 - 30
Опубликована: Апрель 23, 2024
Characterized
by
their
pivotal
roles
in
cell-to-cell
communication,
cell
proliferation,
and
immune
regulation
during
tissue
repair,
exosomes
have
emerged
as
a
promising
avenue
for
"cell-free
therapy"
clinical
applications.
Hydrogels,
possessing
commendable
biocompatibility,
degradability,
adjustability,
physical
properties
akin
to
biological
tissues,
also
found
extensive
utility
engineering
regenerative
repair.
The
synergistic
combination
of
hydrogels
holds
the
potential
not
only
enhance
efficiency
but
collaboratively
advance
repair
process.
This
review
has
summarized
advancements
made
over
past
decade
research
hydrogel-exosome
systems
regenerating
various
tissues
including
skin,
bone,
cartilage,
nerves
tendons,
with
focus
on
methods
encapsulating
releasing
within
hydrogels.
It
critically
examined
gaps
limitations
current
research,
whilst
proposed
future
directions
applications
this
innovative
approach.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(19)
Опубликована: Янв. 25, 2024
Abstract
The
evolution
of
extracellular
vesicle
(EV)
research
has
introduced
nanotechnology
into
biomedical
cell
communication
science
while
recognizing
what
is
formerly
considered
“dust”
as
constituting
an
entirely
new
universe
signaling
particles.
To
display
the
global
EV
landscape,
a
systematic
review
20
364
original
articles
selected
from
all
40
684
EV‐related
records
identified
in
PubMed
2013–2022
performed.
Machine‐learning
used
to
categorize
high‐dimensional
data
and
further
dissected
significant
associations
between
source,
isolation
method,
cargo,
function.
Unexpected
correlations
these
four
categories
indicate
prevalent
experimental
strategies
based
on
cargo
connectivity
with
function
interest
being
associated
certain
sources
or
strategies.
Conceptually
relevant
association
size‐based
protein
uptake
will
guide
strategic
conclusions
enhancing
future
product
development.
Based
this
study,
open‐source
database
built
facilitate
analysis
conventional
AI
tools
identify
additional
causative
interest.
Current Neuropharmacology,
Год журнала:
2023,
Номер
21(9), С. 1964 - 1979
Опубликована: Фев. 17, 2023
Exosomes,
as
nanoscale
biological
vesicles,
have
been
shown
to
great
potential
for
biomedical
applications.
However,
the
low
yield
of
exosomes
limits
their
application.
In
this
review,
we
focus
on
methods
increase
exosome
yield.
Two
main
strategies
are
used
production,
one
is
based
genetic
manipulation
biogenesis
and
release
pathway,
other
by
pretreating
parent
cells,
changing
culture
method
or
adding
different
components
medium.
By
applying
these
strategies,
can
be
produced
a
large
scale
facilitate
practical
application
in
clinic.
Pharmaceutics,
Год журнала:
2023,
Номер
15(2), С. 365 - 365
Опубликована: Янв. 21, 2023
The
article
is
divided
into
several
sections,
focusing
on
extracellular
vesicles’
(EVs)
nature,
features,
commonly
employed
methodologies
and
strategies
for
their
isolation/preparation,
characterization/visualization.
This
work
aims
to
give
an
overview
of
advances
in
EVs’
extensive
nanomedical-drug
delivery
applications.
Furthermore,
considerations
EVs
translation
clinical
application
are
summarized
here,
before
the
review
a
special
kind
vesicles,
ones
derived
from
red
blood
cells
(RBCEVs).
Generally,
employing
as
drug
carriers
means
managing
entities
with
advantageous
properties
over
synthetic
vehicles
or
nanoparticles.
Besides
fact
that
certain
also
reveal
intrinsic
therapeutic
characteristics,
regenerative
medicine,
nanosize,
lipidomic
proteomic
profiles
enable
them
pass
biologic
barriers
display
cell/tissue
tropisms;
indeed,
engineering
can
further
optimize
organ
targeting.
In
second
part
review,
we
focus
our
attention
RBCEVs.
First,
describe
biogenesis
composition
those
naturally
produced
by
(RBCs)
under
physiological
pathological
conditions.
Afterwards,
discuss
current
procedures
isolate
and/or
produce
RBCEVs
lab
load
specific
cargo
exploitation.
Finally,
disclose
most
recent
applications
at
vitro
preclinical
research
level
potential
industrial
conclusion,
be,
near
future,
very
promising
versatile
platform
pharmaceutical
exploitations.
Alzheimer s & Dementia,
Год журнала:
2023,
Номер
19(11), С. 4828 - 4840
Опубликована: Апрель 6, 2023
Abstract
Introduction
Extracellular
vesicles
(EVs)
may
propagate
and
modulate
Alzheimer's
disease
(AD)
pathology.
We
aimed
to
comprehensively
characterize
the
proteome
of
cerebrospinal
fluid
(CSF)
EVs
identify
proteins
pathways
altered
in
AD.
Methods
CSF
were
isolated
by
ultracentrifugation
(Cohort
1)
or
Vn96
peptide
2)
from
non‐neurodegenerative
controls
(
n
=
15,
16)
AD
patients
22,
20,
respectively).
subjected
untargeted
quantitative
mass
spectrometry‐based
proteomics.
Results
validated
enzyme‐linked
immunosorbent
assay
(ELISA)
Cohorts
3
4,
consisting
16,
43,
(Cohort3,
Cohort4)),
with
24,
100).
found
>
30
differentially
expressed
involved
immune‐regulation.
Increase
C1q
levels
compared
non‐demented
was
ELISA
(∼
1.5
fold,
p
3)
0.03,
4)
0.005).
Discussion
be
utilized
as
a
potential
biomarker
play
so
far
unprecedented
role
immune‐regulation
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 7041 - 7041
Опубликована: Июнь 27, 2024
Neuroinflammation,
crucial
in
neurological
disorders
like
Alzheimer's
disease,
multiple
sclerosis,
and
hepatic
encephalopathy,
involves
complex
immune
responses.
Extracellular
vesicles
(EVs)
play
a
pivotal
role
intercellular
inter-organ
communication,
influencing
disease
progression.
EVs
serve
as
key
mediators
the
system,
containing
molecules
capable
of
activating
molecular
pathways
that
exacerbate
neuroinflammatory
processes
disorders.
However,
from
mesenchymal
stem
cells
show
promise
reducing
neuroinflammation
cognitive
deficits.
can
cross
CNS
barriers,
peripheral
signals
influence
brain
function
via
EV-mediated
impacting
barrier
Understanding
EV
interactions
within
other
organs
could
unveil
novel
therapeutic
targets
for
