Aggregation-Induced Emission Luminogen: Role in Biopsy for Precision Medicine

Chemical Reviews, Год журнала: 2024, Номер 124(20), С. 11242 - 11347

Опубликована: Окт. 9, 2024

Biopsy, including tissue and liquid biopsy, offers comprehensive real-time physiological pathological information for disease detection, diagnosis, monitoring. Fluorescent probes are frequently selected to obtain adequate on processes in a rapid minimally invasive manner based their advantages biopsy. However, conventional fluorescent have been found show aggregation-caused quenching (ACQ) properties, impeding greater progresses this area. Since the discovery of aggregation-induced emission luminogen (AIEgen) promoted advancements molecular bionanomaterials owing unique high quantum yield (QY) signal-to-noise ratio (SNR),

Язык: Английский

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Proteomic comparison between non‐purified cerebrospinal fluid and cerebrospinal fluid‐derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia

Journal of Extracellular Vesicles, Год журнала: 2023, Номер 12(12)

Опубликована: Дек. 1, 2023

Abstract Dementia is a leading cause of death worldwide, with increasing prevalence as global life expectancy increases. The most common neurodegenerative disorders are Alzheimer's disease (AD), dementia Lewy bodies (DLB) and Parkinson's (PDD). With this study, we took an in‐depth look at the proteome (non‐purified) cerebrospinal fluid (CSF) CSF‐derived extracellular vesicles (EVs) AD, PD, PD‐MCI (Parkinson's mild cognitive impairment), PDD DLB patients analysed by label‐free mass spectrometry. This has led to discovery differentially expressed proteins that may be helpful for differential diagnosis. We observed greater number in EV samples ( N = 276) compared non‐purified CSF 169), minimal overlap between both datasets. finding suggests more suitable phase biomarker due removal abundant proteins, resulting narrower dynamic range. As disease‐specific markers, selected total 39 candidates identified CSF, 37 across different diseases under investigation data. After further exploration validation these they can used differentiate included dementias offer new avenues research into pharmacological therapeutics.

Язык: Английский

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Proteomics profiling of extracellular vesicle for identification of potential biomarkers in Alzheimer's disease: A comprehensive review

Ageing Research Reviews, Год журнала: 2024, Номер 99, С. 102359 - 102359

Опубликована: Май 29, 2024

Язык: Английский

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Emerging Role of Extracellular Vesicles as Biomarkers in Neurodegenerative Diseases and Their Clinical and Therapeutic Potential in Central Nervous System Pathologies

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 10068 - 10068

Опубликована: Сен. 19, 2024

The emerging role of extracellular vesicles (EVs) in central nervous system (CNS) diseases is gaining significant interest, particularly their applications as diagnostic biomarkers and therapeutic agents. EVs are involved intercellular communication secreted by all cell types. They contain specific markers a diverse cargo such proteins, lipids, nucleic acids, reflecting the physiological pathological state originating cells. Their reduced immunogenicity ability to cross blood-brain barrier make them promising candidates for both In context CNS diseases, have shown promise isolable from different body fluids, providing non-invasive method diagnosing monitoring disease progression. This makes useful early detection Alzheimer's, Parkinson's, amyotrophic lateral sclerosis, where alterations content can be detected. Additionally, derived stem cells show potential promoting tissue regeneration repairing damaged tissues. An evaluation has been conducted on current clinical trials studying focusing application, treatment protocols, obtained results. review aims explore carriers highlighting advantages ongoing evaluating efficacy.

Язык: Английский

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Filter-aided extracellular vesicle enrichment (FAEVEr) for proteomics

Molecular & Cellular Proteomics, Год журнала: 2025, Номер 24(2), С. 100907 - 100907

Опубликована: Янв. 21, 2025

Язык: Английский

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Characterization of RNA cargo from extracellular vesicles obtained from cerebrospinal fluid and plasma samples in schizophrenia participants and healthy volunteers

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Abstract Biomarkers that are clinically useful for the diagnosis and treatment of schizophrenia lacking. critical tools reduce incidence misdiagnosis, identify subgroups patients, assist in proper characterization patient phenotypes, predict response to or development side effects, can serve as targets novel therapeutic interventions. In this study, we evaluated small (< 200 nucleotide) long (> RNAs found extracellular vesicles (EVs) isolated from cerebrospinal fluid (CSF) plasma individuals with spectrum disorders (SSD) healthy volunteers (HV). As EVs carry cargo all tissues body, they act a potential proxy tissue origin, including cells brain. We compared transcriptomic features these two biofluids examined their ability discriminate between SSD HV participants, identifying total 141 differentially expressed genes, some which have been previously associated SSD. Next, cell-types give rise SSD-associated CSF RNA cargo, majority were predominantly excitatory neurons. Our results highlight both source relevant biomarkers, molecular insight into disease mechanisms.

Язык: Английский

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Extracellular vesicles: new horizons in neurodegeneration

EBioMedicine, Год журнала: 2025, Номер 113, С. 105605 - 105605

Опубликована: Март 1, 2025

Extracellular vesicles (EVs) are lipid-enclosed nanovesicles secreted by diverse cell types that orchestrate intercellular communication through cargo delivery. Their pivotal roles span from supporting the development of normal central nervous system (CNS) to contributing pathogenesis neurological diseases. Particularly noteworthy is their involvement in propagation pathogenic proteins, such as those involved neurodegenerative disorders, and nucleic acids, closely linking them disease onset progression. Moreover, EVs have emerged promising diagnostic biomarkers for disorders tools staging, owing ability traverse blood-brain barrier specific, stable, accessible properties. This review comprehensively explores realm CNS-derived found peripheral blood, encompassing detection methods, transport mechanisms, various Furthermore, we evaluate potentials limitations clinical applications highlight prospective research directions this rapidly evolving field.

Язык: Английский

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Therapeutic Approaches and Potential Mechanisms of Small Extracellular Vesicles in Treating Vascular Dementia

Cells, Год журнала: 2025, Номер 14(6), С. 409 - 409

Опубликована: Март 11, 2025

Small extracellular vesicles (sEVs), including exosomes as a subtype, with diameter typically less than 200 nm and originating from the endosomal system, are capable of transporting diverse array bioactive molecules, proteins, nucleic acids, lipids, thereby facilitating intercellular communication modulating cellular functions. Vascular dementia (VaD) represents form cognitive impairment attributed to cerebrovascular disease, characterized by complex multifaceted pathophysiological mechanism. Currently, therapeutic approach VaD predominantly emphasizes symptom management, no specific pharmacological treatment exists cure condition. Recent investigations have illuminated significant role sEVs in pathogenesis vascular dementia. This review seeks provide comprehensive analysis characteristics functions sEVs, particular focus on their involvement its underlying mechanisms. The objective is advance understanding interplays between dementia, offering novel insights for future research strategies.

Язык: Английский

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Alzheimer’s disease and related dementias diagnosis: a biomarkers meta-analysis of general and CNS extracellular vesicles

Опубликована: Март 12, 2025

Abstract Accurate differential diagnosis of Alzheimer’s disease (AD) and related dementias (ADRD) continues to challenge neurologists. Extracellular vesicles (EVs) have emerged as a popular tool for their capacity encapsulate disease-specific signatures, particularly in neurodegenerative neurological disorders. We performed PRISMA-guided systematic review meta-analysis, utilizing sophisticated statistical modeling evaluate the diagnostic accuracy, variance, heterogeneity ( I 2 ), publication bias using biomarkers derived from general EVs n = 44) or speculative CNS-enriched 18). Biomarkers demonstrated superior less heterogeneity, than those EVs. The accuracy was low differentiating among different dementia disorders negative controls. However, analysis distinguishing AD vascular cognitive impairment achieved highest accuracy. Meta-regressions revealed importance several preanalytical factors methods. Lastly, we highlight important guidelines future studies take into consideration.

Язык: Английский

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Exosomes as Biomarkers and Therapeutic Agents in Neurodegenerative Diseases: Current Insights and Future Directions

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Abstract Neurodegenerative diseases (NDs) like Alzheimer’s, Parkinson’s, and ALS rank among the most challenging global health issues, marked by substantial obstacles in early diagnosis effective treatment. Current diagnostic techniques frequently demonstrate inadequate sensitivity specificity, whilst conventional treatment strategies encounter challenges related to restricted bioavailability insufficient blood–brain barrier (BBB) permeability. Recently, exosomes—nanoscale vesicles packed with proteins, RNAs, lipids—have emerged as promising agents potential reshape therapeutic approaches these diseases. Unlike drug carriers, they naturally traverse BBB can deliver bioactive molecules affected neural cells. Their molecular cargo influence cell signaling, reduce neuroinflammation, potentially slow neurodegenerative progression. Moreover, exosomes serve non-invasive biomarkers, enabling precise while allowing real-time disease monitoring. Additionally, engineered exosomes, loaded molecules, enhance this capability targeting diseased neurons overcoming barriers. By offering enhanced reduced immunogenicity, an ability bypass physiological limitations, exosome-based present a transformative advantage over existing approaches. This review examines multifaceted role of NDDs, emphasizing their capabilities, intrinsic functions, advanced vehicles.

Язык: Английский

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