Bacterial extracellular vesicles for gut microbiome-host communication and drug development

Acta Pharmaceutica Sinica B, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

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Harnessing the therapeutic potential of bacterial extracellular vesicles via functional peptides

Deleted Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 23, 2025

Abstract Bacterial extracellular vesicles (BEVs) are nanoscale secreted by bacteria. They possess lipid bilayer membranes and contain multiple periplasmic cytoplasmic components, facilitating their intercellular communications through transferring of various bioactive molecules. Considering intrinsic structure, delivery capacity, immunogenic property, facile production, versatile modification, more studies have exploited BEVs directly as therapeutic agents or engineered vehicles for the disease treatment. Nevertheless, unprecedented upsurge in highlighted burgeoning need tailoring them with enhanced efficacies including specific targeting, subcellular penetration, pathological site retention, so on. With this aspect, functional peptides either penetrating, immunostimulating, biofunctions, self‐assembly exhibit power to open new avenues. Functional can be modified on surface, encapsulated inside membranes, even self‐assembled into hydrogel embedding around fully unleash potential BEVs. Herein, present perspective is dedicated overview most recent advancements exploring bridging treatment, provide valuable insights future development innovative modalities.

Язык: Английский

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Catharanthine tartrate ameliorates osteoclastogenesis by destabilizing HIF-1α

Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111779 - 111779

Опубликована: Март 1, 2025

Язык: Английский

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Identification of the Micro‐Ribonucleic Acid Profiles of Plant‐Derived Extracellular Vesicles and their Potential Crosskingdom Regulation

Advanced NanoBiomed Research, Год журнала: 2025, Номер unknown

Опубликована: Март 30, 2025

Plant‐derived extracellular vesicles (pEVs) are nanosized that have comparable structure and properties to EVs derived from mammalian cells. Prior studies confirmed pEVs remarkable efficacy in the treatment of human diseases, such as cancer. As critical regulators gene expression, microRNAs (miRNAs) abundant pEVs. However, their potential functional roles regulatory mechanisms mediating crosskingdom regulation cells by remain undefined. In particular, similarities differences miRNA profiles various elusive. Herein, isolated grapefruit, ginger, lemon, grape, small RNA (sRNA) libraries constructed perform sRNA sequencing. Only 15 consistently expressed miRNAs identified these Furthermore, top 20 each pEV highly among total miRNAs, accounting for 79.93–87.12%. Through annotation analysis target genes, found be involved regulating progression cancer viral infection. Taken together, this study demonstrates contained play a role effects against genes highlights significant therapeutic applications diseases.

Язык: Английский

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Engineered Pseudomonas mirabilis-Derived Outer Membrane Vesicles Targeting Bone Microenvironment to Improve Osteoporosis

Biomedicines, Год журнала: 2025, Номер 13(4), С. 847 - 847

Опубликована: Апрель 2, 2025

Introduction: Osteoporosis (OP) is a prevalent condition marked by reduced bone density and heightened risk of fractures. Current treatments often have side effects, underscoring the need for safer alternatives. Recent research highlights significant role gut microbiota their metabolites in maintaining health. Notably, bacterial outer membrane vesicles (OMVs) emerged as promising platform due to nanoscale sizes, low toxicity, drug-loading capabilities, excellent biocompatibility. Methods: In this study, we developed delivery system using OMVs derived from Pseudomonas mirabilis (PM). By anchoring bone-targeting peptides PM-OMVs membrane, equipped these deliver endogenous miRNAs microenvironment effectively. Results Discussion: The bone-targeted (PM-OMVs-BT) demonstrated exceptional abilities exhibited favorable safety profile vivo. Additionally, LGG-OMVs-BT were successfully internalized marrow stromal cells (BMSCs) without cytotoxicity, effectively promoting osteogenic differentiation mineralization. conclusion, our study indicates that PM-OMVs-BT could offer safe effective treatment option OP.

Язык: Английский

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Intestinal bacteria-derived extracellular vesicles in metabolic dysfunction-associated steatotic liver disease: from mechanisms to therapeutics

Molecules and Cells, Год журнала: 2025, Номер unknown, С. 100216 - 100216

Опубликована: Апрель 1, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a progressive that affects the health of approximately one-third world's population. It primary cause end-stage disease, malignancy, and transplantation, resulting in great medical burden. No medications have yet been approved by US Food Drug Administration (FDA) for treating MASLD without inflammation or scarring. Therefore, development specific drugs to treat remains key task ongoing research objective. Extracellular vesicles (EVs) play an important role communication between organs, tissues, cells. Recent studies found intestinal microbiota are closely related pathogenesis progression MASLD. EVs produced bacteria (BEVs) indispensable this process. Thus, study provides new direction treatment. However, mechanism which BEVs affect unclear. investigated influence function Additionally, we focus on progress recent years, explain relationship from perspectives glucose lipid metabolism, immune responses, homeostasis. Finally, summarized potential therapeutic value other sources, such as adipocytes, immunocytes, stem cells, plants.

Язык: Английский

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Synthetically Engineered Bacterial Extracellular Vesicles and IL-4-Encapsulated Hydrogels Sequentially Promote Osteoporotic Fracture Repair

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Osteoporosis (OP) is a systemic disease characterized by decreased bone density and quality, leading to fragile bones osteoporotic fractures (OPF). Conventional treatments for OPF often exhibit limited therapeutic efficacy significant side effects. Synthetic biology-based bacterial extracellular vesicles (BEVs) offer safe effective alternative treatment. Here, we constructed bioengineered BEVs loaded with pBMP-2-VEGF (BEVs-BP) encapsulated them together IL-4 in GelMA hydrogels form IL-4/BEVs-BP@GelMA. Initially, alleviated chronic inflammation modulating immune cells, while BEVs-BP subsequently enhanced osteogenesis vascularization upregulating BMP-2 VEGF expression. In vitro, IL-4/BEVs-BP@GelMA polarized M1 macrophages toward the M2 phenotype, osteogenesis, increased angiogenesis. Moreover, effectively promoted maturation mineralization of organoids vivo. Finally, successfully accelerated fracture repair mice. summary, developed an easy-to-build powerful biomaterial, IL-4/BEVs-BP@GelMA, which offers strategy management.

Язык: Английский

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Engineered lung cell targeting and SLC7A11 siRNA expressing bacterial extracellular vesicles impair the progression of none‐small cell lung cancer

Bioengineering & Translational Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Abstract Non‐small cell lung cancer (NSCLC) presents significant therapeutic challenges, often characterized by aggressive proliferation and metastasis. This study investigates the role of SLC7A11, a ferroptosis‐related gene, in NSCLC progression potential engineered bacterial extracellular vesicles (BEVs) expressing SLC7A11‐targeting siRNA as strategy. Using TCGA GEO databases, we identified that SLC7A11 was significantly upregulated tissues. Functional assays demonstrated knockdown lines (NCI‐H2122 NCI‐H647) via qPCR, Western blot, immunofluorescence resulted impaired proliferation, migration, invasion abilities. In vivo xenograft models further revealed inhibited tumor growth metastasis, corroborated histological analyses. To enhance targeted delivery siRNA, BEVs with targeting peptide, verifying their structure function through transmission electron microscopy (TEM) nanoparticle tracking analysis (NTA). toxicity assessments indicated safety for these bioengineered vesicles. Importantly, treatment BEVs‐LCTP‐siSLC7A11 not only tumorigenesis but also activated ferroptosis pathways, evidenced altered expression levels transferrin metastatic Our findings suggest promising approach to inhibit while activating ferroptosis, offering insights into novel strategies against cancer.

Язык: Английский

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Bioengineered bacterial extracellular vesicles for targeted delivery of an osteoclastogenesis-inhibitory peptide to alleviate osteoporosis

Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113751 - 113751

Опубликована: Апрель 1, 2025

Язык: Английский

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Cold exposure-induced plasma exosomes impair bone mass by inhibiting autophagy

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Июнь 23, 2024

Recently, environmental temperature has been shown to regulate bone homeostasis. However, the mechanisms by which cold exposure affects mass remain unclear. In our present study, we observed that (CT) decreased and quality in mice. Furthermore, a transplant of exosomes derived from plasma mice exposed (CT-EXO) can also impair osteogenic differentiation BMSCs decrease inhibiting autophagic activity. Rapamycin, potent inducer autophagy, reverse or CT-EXO-induced loss. Microarray sequencing revealed increases miR-25-3p level CT-EXO. Mechanistic studies showed inhibit activity BMSCs. It is inhibition release downregulation suppress CT-induced This study identifies CT-EXO mediates osteoporotic effects through autophagy via targeting SATB2, presenting novel mechanism underlying effect on mass.

Язык: Английский

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