Cytokine, Год журнала: 2024, Номер 183, С. 156746 - 156746
Опубликована: Сен. 5, 2024
Язык: Английский
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 23, 2025
Articular cartilage injuries can lead to pain, stiffness, and reduced mobility, may eventually progress osteoarthritis (OA). Despite substantial research efforts, effective therapies capable of regenerating are still lacking. Mesenchymal stromal cells (MSCs) known for their differentiation immunomodulatory capabilities, yet challenges such as limited survival post-injection inconsistent therapeutic outcomes hinder clinical application. Recent evidence suggests that the beneficial effects MSCs largely mediated by secreted small extracellular vesicles (sEVs), which have been shown promote tissue repair reduce inflammation. MSC-derived sEVs promise in mitigating degradation chondrocyte apoptosis, positioning them a promising alternative MSC-based OA treatment. This review explores potential limitations regeneration. systematic was conducted following PRISMA guidelines, with comprehensive search Web Science Scopus databases studies published between 2019 2024. A total 223 records were identified, 132 articles assessed eligibility based on general selection criteria. After full-text screening, 60 initially selected, comprising 58 vitro 40 vivo studies. Following further exclusion specific criteria, 33 28 from 47 scientific papers included final qualitative synthesis. Most indicate enhance proliferation, improve matrix composition, matrix-degrading enzymes inflammation, thereby delaying progression. growing body supports use tool preventing progression, most reporting structure function. However, remain optimizing standardizing isolation, dosage, delivery methods Further is necessary elucidate mechanisms underlying sEVs-mediated regeneration facilitate translation into therapies.
Язык: Английский
Процитировано
1
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 870 - 870
Опубликована: Янв. 21, 2025
Osteoarthritis (OA) is a prevalent joint disorder that lacks effective therapies to halt cartilage degeneration. Mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) are being investigated as promising chondroprotective agents. Compared primary MSCs, induced pluripotent stem (iPSC)-derived MSCs (MLCs) offer superior scalability and enhanced paracrine activity. The aim of this study was explore the feasibility using autologous MLC-derived sEVs potential therapeutic strategy for OA through analysis their protein cargo. iPSCs from an patient healthy donor were differentiated into MLCs. isolated these MLCs characterized, with particular focus on Both iPSC lines successfully MLCs, which secreted comparable size distributions yields. differentially expressed proteins revealed high abundance associated pathology degradation in compared those persistence OA-associated signatures may limit efficacy. These findings underscore importance carefully evaluating disease-specific profiles regenerative applications.
Язык: Английский
Процитировано
0
Toxicology and Applied Pharmacology, Год журнала: 2025, Номер unknown, С. 117241 - 117241
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Advanced Functional Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 21, 2025
Abstract Bone defects remain challenging to treat, with common therapies still relying on invasive approaches. Mesenchymal stem cell‐derived extracellular vesicles (MSC‐EVs) offer a promising alternative due their regenerative and immunomodulatory properties, but challenges in scalable EV production limit clinical translation. Nanoghosts (NGs) are an emerging class of EV‐mimetics synthesized through the physical distortion ghost cells that innate bioactivity similar EVs while having more yields. In this study, osteogenic potential NGs made from MSC ghosts (MSC‐NGs) is explored for first time contrasted conventional MSC‐EVs. MSC‐NGs generated sonication, yielding two‐fold compared MSC‐EVs same number cells. Unlike MSC‐EVs, significantly enhanced differentiation MSCs, evidenced by increased alkaline phosphatase (ALP) activity early mineralization. Proteomic analysis further revealed enriched osteogenesis‐related proteins than vivo, treatment 0.5 mm mouse femoral osteotomy accelerated fracture healing, showing callus mineralization day 14 improved bone marrow reconstitution 21, along reduced osteoclastic activity. These findings demonstrate as effective therapeutics tissue engineering, offering advantages over future healing strategies.
Язык: Английский
Процитировано
0
Frontiers in Pharmacology, Год журнала: 2025, Номер 16
Опубликована: Март 13, 2025
Background With the rapid development of extracellular vesicles (EVs) in regenerative medicine research, they have become a promising new direction mechanistic, diagnosis and treatment studies degenerative musculoskeletal diseases (DMDs), has attracted increasing attention. However, there is currently lack comprehensive objective summary analysis to help researchers quickly conveniently understand trajectory future trends this field. Method This study collected articles reviews published from 2006 2024 on EVs DMDs Web Science database. Bibliometric visual was conducted using several tools, including Microsoft Excel Office, VOSviewer, CiteSpace, Pajek, R packages. Results 1,182 publications were included 2024. Notably, increase number starting 2016, suggesting that field remains developmental stage. Co-authorship revealed China ranked first terms publications, whereas United States led citations. The journal with highest International Journal Molecular Sciences (INT J MOL SCI). most prolific authors Ragni, E 23 while cited author Toh, WS. Additionally, nine top 10 institutions China, Shanghai Jiao Tong University leading publications. article “MSC exosomes mediate cartilage repair by enhancing proliferation, attenuating apoptosis modulating immune reactivity”, authored Zhang, S, BIOMATERIALS 2018. Conclusion study, through bibliometric analysis, clearly illustrates collaborative relationships among countries, authors, institutions, journals, providing valuable insights for seeking academic collaboration opportunities. Moreover, keywords citations allows better key research hotspots frontiers field, points toward directions research. growing interest EV over recent years indicates attention dynamic progression
Язык: Английский
Процитировано
0
Cytokine, Год журнала: 2024, Номер 183, С. 156746 - 156746
Опубликована: Сен. 5, 2024
Язык: Английский
Процитировано
2