The proteome of circulating extracellular vesicles and their functional effect on platelets vary with the isolation method DOI Creative Commons

Eva M Fernández-Sáez,

Susana B. Bravo, Carmen Pena

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

Abstract Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and serve as source of biomarkers several pathologies. In this study, we aimed to characterize plasma-derived EVs isolated by ultracentrifugation (UC) or size exclusion chromatography (SEC) define the best method for proteomic functional studies. characterization included nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), detection western blotting, quantitative analysis. SEC-EVs samples had higher particle protein concentration, particle-to-protein ratio, smaller compared UC-EVs. A total 171 proteins were identified through Sequential Window Acquisition All Theoretical Mass Spectra (SWATH-MS) analysis, with 11 increased 5 The UC-EVs are complement immunoglobulins, while apolipoproteins present extracellular space. Functional studies from activated platelets confirmed that SEC exacerbate platelet aggregation, whereas there was no effect induced latter suggests obtained seem more suitable platelet-related those UC. results presented pave way future clinical orientated involving EVs.

Язык: Английский

Application of Multiomics Approach to Investigate the Therapeutic Potentials of Stem Cell-derived Extracellular Vesicle Subpopulations for Alzheimer's Disease DOI Creative Commons
Morteza Abyadeh, Alaattin Kaya

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 14, 2024

Alzheimer's disease (AD) presents a complex interplay of molecular alterations, yet understanding its pathogenesis remains challenge. In this study, we delved into the intricate landscape proteome and transcriptome changes in AD brains compared to healthy controls, examining 788 brain samples revealing common alterations at both protein mRNA levels. Moreover, our analysis revealed distinct protein-level aberrant energy metabolism pathways that were not evident level. This suggests expression could provide deeper representation pathogenesis. Subsequently, using comparative proteomic approach, explored therapeutic potential mesenchymal stem cell-derived extracellular vehicles (EVs), isolated through various methods, mitigating AD-associated Our particular EV-subtype can be utilized for compensating dysregulated mitochondrial proteostasis brain. By network biology approaches, further regulators key proteins. Overall, study illuminates significance identifies promise specific EV subpopulation with reduced pro-inflammatory cargo enriched proteins target proteostasis.

Язык: Английский

Процитировано

0
The proteome of circulating extracellular vesicles and their functional effect on platelets vary with the isolation method DOI Creative Commons

Eva M Fernández-Sáez,

Susana B. Bravo, Carmen Pena

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

Abstract Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and serve as source of biomarkers several pathologies. In this study, we aimed to characterize plasma-derived EVs isolated by ultracentrifugation (UC) or size exclusion chromatography (SEC) define the best method for proteomic functional studies. characterization included nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), detection western blotting, quantitative analysis. SEC-EVs samples had higher particle protein concentration, particle-to-protein ratio, smaller compared UC-EVs. A total 171 proteins were identified through Sequential Window Acquisition All Theoretical Mass Spectra (SWATH-MS) analysis, with 11 increased 5 The UC-EVs are complement immunoglobulins, while apolipoproteins present extracellular space. Functional studies from activated platelets confirmed that SEC exacerbate platelet aggregation, whereas there was no effect induced latter suggests obtained seem more suitable platelet-related those UC. results presented pave way future clinical orientated involving EVs.

Язык: Английский

Процитировано

0