Advancing nuclear transfer cloning in zebrafish (Danio rerio) into a translational pathway using interdisciplinary tools DOI Creative Commons
Sarah Bodenstein,

William Poulos,

Francisco Jiménez

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(12), С. e0312672 - e0312672

Опубликована: Дек. 30, 2024

The Zebrafish International Resource Center (ZIRC) is an NIH-funded national stock center and germplasm repository that maintains distributes genetically modified wild-type zebrafish ( Danio rerio ) lines to the biomedical research community. ZIRC its community would benefit from incorporating somatic cell nuclear transfer (SCNT) cloning which allow preservation of diploid genomes. goal this study was advance a SCNT protocol into reproducible community-level pathway by use process mapping simulation modeling approaches address training requirements, constraints, quality management gaps. Training, for most steps in protocol, could be completed within two months; however, involved micromanipulation eggs required more than four months training. Dechorionation embryos egg were identified as major constraints because processes performed manually advanced operator manual skills. Chemical dechorionation microfluidic devices aid ways eliminate these constraints. Finally, control record initial collected recommended prevent production defects harmonize across multiple facilities. By beginning enhance reproducibility pathway, technique can implemented facilities work with other models.

Язык: Английский

Advanced sperm preservation techniques in yellow spotted mountain newts Neurergus derjugini enhance genetic management and conservation efforts DOI Creative Commons

Zeynab Taheri-Khas,

Ahmad Gharzi, Somaye Vaissi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 18, 2025

Advances in cold storage and cryopreservation of amphibian sperm are critical for the genetic management conservation threatened species. This study represents first investigation into yellow spotted mountain newt (Neurergus derjugini), focusing on both short-term long-term future reproductive efforts. We examined effects seven extenders motility over time at three temperatures (4 ± 1 °C, 9 20 °C). Additionally, we assessed impact 16 cryoprotectants morphology post-thawing. Following identification most effective freezing medium, evaluated DNA fragmentation to ensure viability. Our results indicate that 10% Holtfreter's solution is optimal extender all temperatures, maintaining up 15 days 4 °C. For storage, a combination DMSO was found best preserve motility, morphology, minimize after thawing. These findings underscore importance specific temperature treatments enhancing functionality, thereby supporting successful assisted technologies (ART) endangered

Язык: Английский

Процитировано

0
Trawling aquatic life for new models in biomedical research and evolutionary developmental biology DOI
Patricia N. Schneider, Frauke Seemann, Matthew P. Harris

и другие.

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Год журнала: 2024, Номер 342(3), С. 123 - 125

Опубликована: Апрель 30, 2024

Язык: Английский

Процитировано

0
Advancing nuclear transfer cloning in zebrafish (Danio rerio) into a translational pathway using interdisciplinary tools DOI Creative Commons
Sarah Bodenstein,

William Poulos,

Francisco Jiménez

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(12), С. e0312672 - e0312672

Опубликована: Дек. 30, 2024

The Zebrafish International Resource Center (ZIRC) is an NIH-funded national stock center and germplasm repository that maintains distributes genetically modified wild-type zebrafish ( Danio rerio ) lines to the biomedical research community. ZIRC its community would benefit from incorporating somatic cell nuclear transfer (SCNT) cloning which allow preservation of diploid genomes. goal this study was advance a SCNT protocol into reproducible community-level pathway by use process mapping simulation modeling approaches address training requirements, constraints, quality management gaps. Training, for most steps in protocol, could be completed within two months; however, involved micromanipulation eggs required more than four months training. Dechorionation embryos egg were identified as major constraints because processes performed manually advanced operator manual skills. Chemical dechorionation microfluidic devices aid ways eliminate these constraints. Finally, control record initial collected recommended prevent production defects harmonize across multiple facilities. By beginning enhance reproducibility pathway, technique can implemented facilities work with other models.

Язык: Английский

Процитировано

0