The effect of dietary emulsifiers and thickeners on intestinal barrier function and its response to acute stress in healthy adult humans: A randomised controlled feeding study
Alimentary Pharmacology & Therapeutics,
Год журнала:
2024,
Номер
60(7), С. 863 - 875
Опубликована: Июль 27, 2024
Summary
Background
Although
dietary
emulsifiers
are
implicated
in
the
pathogenesis
of
Crohn's
disease,
their
effect
has
not
been
studied
humans.
Aim
To
determine
effects
high‐
and
low‐emulsifier
diets
(HED,
LED)
on
intestinal
barrier
function
healthy
subjects
unstressed
acutely
stressed
states.
Methods
We
conducted
a
single‐blinded,
cross‐over,
controlled
feeding
trial
22
adults.
After
recording
7
days
habitual
diet,
we
randomised
participants
to
HED
or
LED
with
≥3‐week
washout
between
diets.
On
completion,
acute
stress
was
induced
via
intravenous
corticotrophin‐releasing
hormone.
assessed
adherence,
2‐h
urinary
lactulose:
rhamnose
ratio
(LRR),
serum
concentrations
lipopolysaccharide‐binding
protein,
soluble‐CD14
markers
epithelial
injury
inflammation.
Results
Dietary
adherence
excellent.
In
an
state,
median
(interquartile
range)
LRR
during
0.030
(0.018–0.042);
LED,
this
0.042
(0.029–0.078;
p
=
0.04).
LPB
were
lower
than
(
0.026),
but
no
differences
observed
for
Under
stress,
increased
by
89%
(−1%
486%)
0.004),
differing
0.001)
from
39%
(1%–90%)
decrease
0.009).
Soluble‐CD14
also
<
0.001).
The
had
prolonged
carry‐over
suppressing
HED‐induced
changes
stress.
Similar
when
used
as
first
diet
(both
0.01).
Conclusion
High
intake
improved
permeability
without
evidence
A
protective
effect.
Язык: Английский
Review article: Evidence‐based dietary management of inflammatory bowel disease
Alimentary Pharmacology & Therapeutics,
Год журнала:
2024,
Номер
60(9), С. 1215 - 1233
Опубликована: Авг. 20, 2024
Summary
Background
Dietary
management
of
patients
with
inflammatory
bowel
disease
(IBD)
involves
more
than
defining
a
therapeutic
diet.
The
profusion
‘expert
advice’
is
not
necessarily
built
on
evidence.
Aims
To
provide
evidence‐based
guidance
all
clinically
relevant
aspects
nutritional
and
dietary
IBD.
Methods
A
comprehensive
review
the
published
literature
was
made.
Results
Four
pillars
should
be
considered
in
patients.
First,
status
optimised,
since
myopenia
visceral
obesity
are
associated
poorer
outcomes,
which
can
improved
attention
to
their
correction.
Accurate
point‐of‐care
measurement
body
composition
advocated
identify
problems,
guide
interventions
monitor
outcomes.
Second,
exclusive
enteral
nutrition
Crohn's
Disease
Exclusion
Diet
partial
reducing
intestinal
inflammation
have
sufficient
evidence
advocated.
Multiple
other
approaches,
while
promising,
insufficient
recommended.
Third,
approaches
important
symptomatic
control
many
non‐inflammatory
scenarios.
Finally,
following
healthy
diet
fundamental
general
health
advocated,
but
optimal
strategy
unclear.
precarious
IBD
together
risks
inadequacy
maladaptive
eating
behaviours
restrictive
diets
dictate
involvement
expert
dietitians
assessment
personalised
delivery
interventions.
Conclusions
require
specific
interventional
strategies
that
chosen
by
Optimal
requires
skills
specialised
dietitian.
Язык: Английский
Integrating the lactulose-mannitol test for intestinal permeability with untargeted metabolomics for drug monitoring through dual liquid chromatography-mass spectrometry
Analytical and Bioanalytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 27, 2025
In
this
study,
we
developed
a
customized
high-resolution
mass
spectrometry
metabolomics
workflow
integrating
the
dual
sugar
test
employing
lactulose
and
mannitol
as
probes
for
intestinal
permeability
assessment
with
untargeted
screening
of
small
molecules.
Urine
samples
were
collected
from
patients
major
depression
healthy
controls
part
clinical
study
at
psychiatric
department.
Using
injection/dual
chromatography
setup,
quantified
by
hydrophilic
interaction
liquid
(HILIC)
in
targeted
assay,
while
drugs
their
metabolites
profiled
an
manner
reversed-phase
separation.
Rigorous
method
development
validation
allowed
selective
separation
isomers
consequently
accurate
quantification
urine.
Internal
standardization
compound
specific
stable
isotope-labeled
standards
enabled
excellent
analytical
figures
merit
such
high
recoveries,
precision
(<
5%),
working
range
(5
orders
magnitude).
Within
one
run,
was
assessed
together
metabolites,
allowing
to
screen
confounding
patient
compliance
therapeutic
scheme.
Язык: Английский
Dietary intake in healthy older individuals is associated with lipopolysaccharide binding protein a biomarker of gut function: an exploratory cross-sectional study
Frontiers in Aging,
Год журнала:
2025,
Номер
6
Опубликована: Март 31, 2025
Diet,
physical
function
and
gut
health
are
important
modifiable
factors
in
ageing.
However,
it
is
unclear
how
ageing
affects
various
domains
of
function.
Aims
this
cross-sectional
study
were
to
explore
relationships
between
nutrient
intake,
function,
biomarkers
older
individuals.
Healthy
participants
(n
=
94,
mean
age
71.1
years
SD
5.10,
56%
female)
recruited
investigate
the
relationship
intake
(protein,
fibre,
carbohydrate,
fat),
(chair
rise
time,
handgrip
strength)
lipopolysaccharide
(LPS)
binding
protein
(LBP);
a
marker
permeability.
Linear
regression
models,
adjusted
for
age,
fat
mass/fat
free
mass
ratio,
weight
gender,
reported
LBP
changed
by;
-161.9
ng/mL
(95%
CI
-323.0,
-0.8)
every
1
g
increase
daily
fibre/1,000
kilocalories;
80.5
(6.7,
154.2)
1%
energy
as
fat;
-88.1
(-146.7,
-29.6)
carbohydrates.
When
further
C-reactive
(CRP),
inflammation,
decreased
by
an
additional
6.9
increased
4.0
3.7
carbohydrate.
These
findings
suggest
that
healthy
adults'
associated
with
LBP,
CRP
appears
slightly
modify
these
associations.
There
no
associations
strength
or
chair
time.
Results
fat,
carbohydrates
maintaining
potentially
mediated
inflammation
adults,
although
research
needed
implications
mediator.
Язык: Английский
Recent advances in measuring the effects of diet on gastrointestinal physiology: Sniffing luminal gases and fecal volatile organic compounds
JGH Open,
Год журнала:
2024,
Номер
8(8)
Опубликована: Июль 29, 2024
Abstract
Despite
the
huge
pool
of
ideas
on
how
diet
can
be
manipulated
to
ameliorate
or
prevent
illnesses,
our
understanding
specific
changes
in
influence
gastrointestinal
tract
is
limited.
This
review
aims
describe
two
innovative
investigative
techniques
that
are
helping
lift
veil
mystery
about
workings
gut.
First,
gas‐sensing
capsule
a
telemetric
swallowable
device
provides
unique
information
gastric
physiology,
small
intestinal
microbial
activity,
and
fermentative
patterns
colon.
Its
ability
accurately
measure
regional
whole‐gut
transit
times
ambulant
humans
has
been
confirmed.
Luminal
concentrations
hydrogen
carbon
dioxide
measured
by
sampling
through
tract,
such
application
enabled
mapping
relative
amounts
fermentation
carbohydrates
proximal‐
versus
‐distal
colon
after
manipulation
types
dietary
fiber.
Second,
smell
feces,
via
analysis
volatile
organic
compounds,
occur
response
diet,
presence
therapy
irritable
bowel
syndrome
inflammatory
disease.
Such
likely
aid
what
change
do
colonic
luminal
microenvironment,
may
value‐add
diagnosis
therapeutic
design.
In
conclusion,
methodologies
enable
more
complete
physiological
profile
created.
Systematic
description
various
cohorts
effects
interventions,
particularly
when
co‐ordinated
with
microbiome,
needed.
Язык: Английский