Comparative Analysis of Host Cell Entry Efficiency and Neutralization Sensitivity of Emerging SARS-CoV-2 Lineages KP.2, KP.2.3, KP.3, and LB.1 DOI Creative Commons

Nianzhen Chen,

Katharina Decker,

Sebastian Schulz

и другие.

Vaccines, Год журнала: 2024, Номер 12(11), С. 1236 - 1236

Опубликована: Окт. 30, 2024

New SARS-CoV-2 lineages continue to evolve and may exhibit new characteristics regarding host cell entry efficiency potential for antibody evasion. Here, employing pseudotyped particles, we compared the efficiency, ACE2 receptor usage, sensitivity antibody-mediated neutralization of four emerging lineages, KP.2, KP.2.3, KP.3, LB.1. The XBB.1.5 JN.1 served as controls. Our findings reveal that LB.1 enter cells efficiently in an ACE2-dependent manner, KP.3 is more adept at entering Calu-3 lung than JN.1. However, variants differed their capacity employ orthologues from animal species entry, suggesting differences interactions. Moreover, demonstrate only two out seven therapeutic monoclonal (mAbs) preclinical development retain robust neutralizing activity against sublineages tested, while three mAbs displayed strongly reduced lacked any tested. Furthermore, our results show evade by antibodies induced infection or vaccination with greater JN.1, particularly individuals without hybrid immunity. This study indicates differ interactions suggest evasion drove emergence these variants.

Язык: Английский

Reverse mutational scanning of spike BA.2.86 identifies the epitopes contributing to immune escape from polyclonal sera DOI Creative Commons
Najat Bdeir,

Tatjana Lüddecke,

Henrike Maaß

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 3, 2024

ABSTRACT The recently detected Omicron BA.2.86 lineage contains more than 30 amino acid mutations relative to BA.2. and its JN.1 derivative evade neutralization by serum antibodies of fully vaccinated individuals. In this study, we elucidate epitopes driving the immune escape via pseudovirus neutralization. Thus, have generated 33 mutants, each reverting a single mutation back We use library in an approach that call reverse mutational scanning define distinct titers against epitope. Mutations within receptor binding domain at K356T, V483Δ, lesser extent N460K, A484K, F486P enhance escape. Interestingly, 16insMPLF spike N-terminal P621S S1/S2 also significantly contribute antibody BA.2.86. Upon XBB.1.5 booster vaccination, improve considerably, residual is driven 16insMPLF, E554K, P621S, A484K. EDITOR’S SUMMARY SARS COV2 has over compared parental BA.2 lineage. Here Bdeir colleagues apply determine which among these present are linked from recognition.

Язык: Английский

Процитировано

5
Reverse mutational scanning of SARS-CoV-2 spike BA.2.86 identifies epitopes contributing to immune escape from polyclonal sera DOI Creative Commons
Najat Bdeir,

Tatjana Lüddecke,

Henrike Maaß

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 18, 2025

The recently detected Omicron BA.2.86 lineage contains more than 30 amino acid mutations relative to BA.2. and its JN.1 derivative evade neutralization by serum antibodies of fully vaccinated individuals. In this study, we elucidate epitopes driving the immune escape via pseudovirus neutralization. Here generate 33 mutants, each reverting a single mutation back We use library in an approach that call reverse mutational scanning define distinct titers against epitope. Mutations within receptor binding domain at K356T, V483Δ, lesser extent N460K, A484K, F486P enhance escape. Interestingly, 16insMPLF spike N-terminal P621S S1/S2 also significantly contribute antibody BA.2.86. Upon XBB.1.5 booster vaccination, improve considerably, residual is driven 16insMPLF, E554K, P621S, A484K.

Язык: Английский

Процитировано

0
Five‐Year (2017–2022) Evolutionary Dynamics of Human Coronavirus HKU1 in Southern France With Emergence of Viruses Harboring Spike H512R Substitution DOI Creative Commons

Houmadi Hikmat,

Lorlane Le Targa,

Céline Boschi

и другие.

Journal of Medical Virology, Год журнала: 2025, Номер 97(2)

Опубликована: Фев. 1, 2025

ABSTRACT HCoV‐HKU1 diversity and evolution were scarcely studied. We performed next‐generation sequencing (NGS) analysis of genomes over 5 years. NGS used Illumina technology on NovaSeq 6000 following whole genome PCR amplification by an in‐house set primers designed using Gemi PrimalScheme. Genome assembly analyses CLC Genomics, Mafft, BioEdit, Nextstrain, Nextclade, MEGA, iTol bioinformatic tools. Spike molecular modeling dynamics simulations Molegro Molecular Viewer Hyperchem programs. Twenty‐eight systems allowed obtaining 158 including 69 89 genotypes A B, respectively. Both co‐circulated during the study period but one predominated each year. total 1683 amino acid substitutions 80 in ≥ 10 detected genotype relatively to a 2004 reference. H512R spike, first 2009 reported as involved antibody neutralization, was found all A, almost always with V387I K478N, predicted here significantly improve cellular TMPRSS2 protein binding. Also, 1802 64 B 2005 This substantially expands global genomes. Genomics structural contributed our understanding evolution.

Язык: Английский

Процитировано

0
Incursion of SARS-CoV-2 BA.2.86.1 variant into Israel: National-scale wastewater surveillance using a novel quantitative real-time PCR assay DOI
Oran Erster,

Itay Bar-Or,

Roberto Azar

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 933, С. 173164 - 173164

Опубликована: Май 11, 2024

Язык: Английский

Процитировано

2
Comparative Analysis of Host Cell Entry Efficiency and Neutralization Sensitivity of Emerging SARS-CoV-2 Lineages KP.2, KP.2.3, KP.3, and LB.1 DOI Creative Commons

Nianzhen Chen,

Katharina Decker,

Sebastian Schulz

и другие.

Vaccines, Год журнала: 2024, Номер 12(11), С. 1236 - 1236

Опубликована: Окт. 30, 2024

New SARS-CoV-2 lineages continue to evolve and may exhibit new characteristics regarding host cell entry efficiency potential for antibody evasion. Here, employing pseudotyped particles, we compared the efficiency, ACE2 receptor usage, sensitivity antibody-mediated neutralization of four emerging lineages, KP.2, KP.2.3, KP.3, LB.1. The XBB.1.5 JN.1 served as controls. Our findings reveal that LB.1 enter cells efficiently in an ACE2-dependent manner, KP.3 is more adept at entering Calu-3 lung than JN.1. However, variants differed their capacity employ orthologues from animal species entry, suggesting differences interactions. Moreover, demonstrate only two out seven therapeutic monoclonal (mAbs) preclinical development retain robust neutralizing activity against sublineages tested, while three mAbs displayed strongly reduced lacked any tested. Furthermore, our results show evade by antibodies induced infection or vaccination with greater JN.1, particularly individuals without hybrid immunity. This study indicates differ interactions suggest evasion drove emergence these variants.

Язык: Английский

Процитировано

1