Diagnostics,
Год журнала:
2024,
Номер
14(13), С. 1448 - 1448
Опубликована: Июль 7, 2024
Head
and
neck
cancer
(HNC)
represents
a
significant
global
health
challenge,
with
squamous
cell
carcinomas
(SCCs)
accounting
for
approximately
90%
of
all
HNC
cases.
These
malignancies,
collectively
referred
to
as
head
carcinoma
(HNSCC),
originate
from
the
mucosal
epithelium
lining
larynx,
pharynx,
oral
cavity.
The
primary
risk
factors
associated
HNSCC
in
economically
disadvantaged
nations
have
been
chronic
alcohol
consumption
tobacco
use.
However,
more
affluent
countries,
landscape
has
shifted
identification
human
papillomavirus
(HPV)
infection,
particularly
HPV-16,
major
factor,
especially
among
nonsmokers.
Understanding
evolving
distinct
biological
behaviors
HPV-positive
HPV-negative
is
critical
developing
targeted
treatment
strategies
improving
patient
outcomes
this
complex
diverse
group
cancers.
Accurate
diagnosis
essential
comprehensive
model
that
integrates
molecular
characteristics,
immune
microenvironment,
clinical
outcomes.
aim
review
was
summarize
current
knowledge
advances
DNA,
RNA,
protein
biomarkers
bodily
fluids
tissues
introduced
new
possibilities
minimally
or
non-invasive
diagnosis,
monitoring,
assessment
therapeutic
responses.
Infectious Agents and Cancer,
Год журнала:
2025,
Номер
20(1)
Опубликована: Март 7, 2025
Cervical
cancer
is
a
significant
public
health
concern,
disproportionately
affecting
women
in
less
developed
regions
due
to
limited
access
screening
and
vaccination
programs.
Despite
advancements
cervical
prevention
treatment,
there
remains
need
for
efficient
cost-effective
diagnostic
tools.
This
study
aimed
develop
multiplex
real-time
PCR
assay
rapidly
accurately
identify
15
human
papillomavirus
(HPV)
genotypes.The
primary
objective
was
design
method
capable
of
simultaneously
detecting
HPV
types
16
18,
which
account
over
70%
cancers,
as
well
other
clinically
relevant
high-risk
probable/possible
high-risk.
To
validate
the
assay's
performance,
we
compared
its
results
with
those
obtained
using
commercially
available
INNO-LiPA
Genotyping
Extra
II
Assay
kit
(FujireBio,
Tokyo,
Japan).
The
successfully
identified
genotypes
single
reaction.
Analysis
150
positive
40
negative
clinical
samples
demonstrated
excellent
concordance
between
two
assays.
in-house
test
exhibited
sensitivity
98%
specificity
100%,
indicating
reliability
accuracy
genotyping.
tool
screening,
multiple
simultaneously.
It
enhances
efficiency
accuracy,
improving
early
detection
management
HPV-related
diseases.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 29, 2025
Purpose
The
persistent
infection
of
high-risk
HPV
(HR-HPV)
is
intricately
linked
to
the
onset
and
progression
cervical
cancer.
This
research
endeavored
develop
a
high-throughput
2D
PCR
method
for
closed-tube
genotyping
11
HR-HPVs.
Methods
Base-quenched
probes
were
specifically
designed
FAM,
VIC,
CY5
channels.
system
underwent
optimization,
with
its
detection
performance
assessed
in
terms
specificity
sensitivity.
Plasmid
mixtures
was
used
simulate
multiple
infections
HPV,
providing
preliminary
insights
into
efficacy
throughput
technology.
Ultimately,
capability
this
using
clinical
samples.
Results
sequenced
tags,
when
paired
primers,
could
generate
Tm
differences
exceeding
3°C.
These
then
integrated
fluorescent
channel
differentiate
identify
target
genes
upon
detection.
refined
confirmed
be
free
from
cross-reactions
exhibited
high
specificity,
capable
detecting
12
within
single
tube.
A
total
294
exfoliated
cell
samples
tested
flow
fluorescence
hybridization
method.
overall
concordance
between
two
methods
96.17%
(Kappa
=
0.910).
Conclusion
method,
which
integrates
asymmetric
amplification
melting
curve
analysis,
has
capacity
detect
types
HR-HPVs
across
three
approach
offers
several
benefits
including
throughput,
straightforward
operation,
low
cost.
Consequently,
it
can
effectively
utilized
early
screening
prevention
Journal of Medical Virology,
Год журнала:
2025,
Номер
97(3)
Опубликована: Март 1, 2025
ABSTRACT
Human
papillomavirus
type
16
(HPV‐16)
is
a
key
driver
in
the
development
of
cervical
carcinoma,
with
integration
its
genome
into
host
DNA
marking
critical
step
disease
progression.
Monitoring
physical
state
HPV‐16,
particularly
transition
from
episomal
to
integrated
forms,
essential
for
evaluating
risk
malignancy
cervix.
This
study
presents
duplex
electrochemical
biosensor
simultaneous
detection
E2
and
E6
genes
HPV‐16.
Using
one‐step
sandwich
hybridization
assay,
was
able
detect
HPV‐16
sensitivity
8
copies/mL
12
respectively
distinguish
between
forms
based
on
E2/E6
ratio
(cut‐off
0.77,
100%
sensitivity/specificity).
The
sensor
validated
30
clinical
tissue
samples,
providing
results
comparable
qPCR
method.
novel
offers
rapid
efficient
platform
monitoring
potential
applications
cancer
screening
prognosis.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 31, 2025
Abstract
The
early
detection
of
high‐risk
human
papillomavirus
(HR‐HPV)
is
crucial
for
the
assessment
and
improvement
prognosis
in
cervical
cancer.
However,
existing
PCR‐based
screening
methods
suffer
from
inadequate
accessibility,
which
dampens
enthusiasm
among
grassroots
populations,
especially
resource‐limited
areas,
contributes
to
persistently
high
mortality
rate
Here,
a
portable
system
proposed
multiplexed
nucleic
acid
detection,
termed
R‐CHIP
,
that
integrates
R
ecombinase
polymerase
amplification
(RPA),
C
RISPR
H
and‐driven
microfluidics,
an
artificial
I
ntelligence
P
latform.
can
go
sample
pre‐processing
results
readout
less
than
hour
with
simple
manual
operation.
Optimized
sensitivity
10
−17
M
HPV‐16
−18
HPV‐18,
has
accuracy
over
95%
300
tests
on
clinical
samples.
In
addition,
smartphone
microimaging
combined
ResNet‐18
deep
learning
model
used
improve
efficiency
convenience
system,
initial
prediction
accuracies
96.0%
98.0%
respectively.
R‐CHIP,
as
user‐friendly
intelligent
platform,
great
potential
community‐level
HR‐HPV
resource‐constrained
settings,
prevention
diagnosis
other
diseases.
Journal of Medical Virology,
Год журнала:
2024,
Номер
96(10)
Опубликована: Окт. 1, 2024
Abstract
Human
papillomaviruses
(HPVs)
represent
a
diverse
group
of
double‐stranded
DNA
viruses
associated
with
various
types
cancers,
notably
cervical
cancer.
High‐risk
HPVs
exhibit
their
oncogenic
potential
through
the
integration
into
host
genome.
This
event
contributes
significantly
to
genomic
instability
and
progression
malignancy.
However,
traditional
detection
methods,
such
as
immunohistochemistry
or
PCR‐based
assays,
face
inherent
challenges,
thus
alternative
tools
are
being
developed
fasten
simplify
analysis.
Our
study
introduces
an
innovative
biosensing
platform
that
combines
loop‐mediated
amplification
electrochemical
(EC)
analysis
for
specific
HPV16
integration.
By
targeting
key
elements
like
E7
mRNA,
central
player
in
HPV
integration,
E2
viral
gene
transcript
lost
upon
we
show
clear
distinction
between
episomal
integrated
forms
HPV16.
EC
data
confirmed
higher
expression
HPV16‐positive
cell
lines
having
genome,
while
was
diminished
cells
fully
genomes.
Moreover,
revealed
distinct
patterns
tissue
patients,
correlating
well
digital
droplet
PCR,
qRT‐PCR,
immunohistochemical
staining.
offers
insights
clinical
samples
facilitates
further
advancements
cancer
research
diagnostics.
International Journal of Surgery,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 16, 2024
Cervical
cancer
ranks
as
the
fourth
most
common
among
women
globally,
posing
a
significant
mortality
risk.
Persistent
infection
with
high-risk
human
papillomavirus
(HPV)
is
primary
instigator
of
cervical
development,
often
alongside
co-infection
other
viruses,
precipitating
various
malignancies.
This
study
aimed
to
explore
recent
biotechnological
advances
in
understanding
HPV
dynamics,
host
interactions,
and
its
role
oncogenesis.
The
gathered
data
shed
light
on
biology,
host-virus
interplay,
viral
co-infections,
cellular
transformations
leading
HPV-associated
cancers.
Recent
years
have
seen
introduction
diverse
vaccination
strategies,
including
live
attenuated,
subunit,
DNA-based
vaccines,
complemented
by
innovative
nanotechnology
plant-based
products.
Despite
rich
addressing
research
inquiries,
urgent
calls
echo
for
implementation
contemporary
screening
therapeutic
modalities
at
clinical
levels.
Moreover,
extensive
public
awareness
campaigns
are
imperative
alleviate
burden
HPV-related
diseases,
emphasizing
necessity
proactive
intervention
strategies
combating
this
global
health
challenge.
Pathogens,
Год журнала:
2024,
Номер
13(8), С. 653 - 653
Опубликована: Авг. 2, 2024
The
human
papillomavirus
(HPV)
is
a
non-enveloped
DNA
virus
transmitted
through
skin-to-skin
contact
that
infects
epithelial
and
mucosal
tissue.
It
has
over
200
known
genotypes,
classified
by
their
pathogenicity
as
high-risk
low-risk
categories.
High-risk
HPV
genotypes
are
associated
with
the
development
of
different
types
cancers,
including
cervical
cancer,
which
leading
cause
mortality
in
women.
In
clinical
practice
market,
principal
tests
used
to
detect
based
on
cytology,
hybrid
detection,
qPCR.
However,
these
methodologies
may
not
be
ideal
for
required
timely
diagnosis.
Tests
have
been
developed
isothermal
nucleic
acid
amplification
(INAATs)
alternatives.
These
offer
multiple
advantages
qPCR,
such
requiring
specialized
laboratories,
highly
trained
personnel,
or
expensive
equipment
like
thermocyclers.
This
review
analyzes
INAATs
applied
detection
HPV,
considering
specific
characteristics
each
test,
gene
target,
limit
(LOD),
methods,
time.
Additionally,
we
discuss
available
market
approved
Food
Drug
Administration
(FDA).
Finally,
address
challenges
potential
solutions
large-scale
implementation
INAATs,
particularly
rural
underserved
areas.
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 25, 2024
Abstract
High‐risk
human
papillomavirus
(HPV)
screening
is
crucial
for
cervical
cancer
prevention.
However,
laboratory‐based
nucleic
acid
amplification
tests
(NAATs)
require
costly
equipment,
designated
lab
space,
and
skilled
personnel.
Additionally,
swabs
collected
by
healthcare
professionals
can
be
inconvenient,
uncomfortable,
reduce
privacy,
limiting
broader
application
patient
compliance.
A
SlipChip‐based
Integrated
Point‐of‐Care
(SIPOC)
system
featuring
an
injection‐molded
SlipChip
presented
with
preloaded
reagents
extraction
a
portable
four‐channel
real‐time
quantitative
PCR
instrument
detection.
This
incorporates
self‐sampling
method
that
allows
participants
to
collect
their
own
vaginal
swabs,
the
β‐Globin
gene
as
control.
After
testing
130
HPV‐16
HPV‐18,
97.7%
of
self‐collected
samples
are
valid.
Among
valid
samples,
25
tested
positive
9
HPV‐18.
Compared
Roche's
standard
HPV
test,
SIPOC
shows
100%
predictive
value
(PPV)
both
HPV‐18
negative
values
(NPVs)
99.0%
99.1%,
respectively.
promising
in
resource‐limited
settings
adaptable
other
point‐of‐care
NAAT
applications,
including
home
testing.
