Journal of Liquid Chromatography & Related Technologies,
Год журнала:
2023,
Номер
47(1-5), С. 1 - 13
Опубликована: Дек. 27, 2023
Qingwei
Huanglian
Wan
(QHW)
is
a
traditional
Chinese
medicine
used
for
treating
inflammation.
As
the
compositions
of
QHW
preparations
are
complex
and
varied,
it
has
proven
difficult
to
identify
active
ingredients
in
control
quality.
In
this
study,
high-performance
liquid
chromatography
(HPLC)
fingerprints,
network
pharmacology,
molecular
docking,
Griess
method
were
screen
verify
potential
QHW.
First,
sixteen
batches
studied
as
two
groups
using
multivariate
statistical
analysis.
Thirteen
peaks
detected
HPLC
results
establish
fingerprint
similarity
model,
six
chemical
constituents
(phellodendrine
hydrochloride,
geniposide,
berberine
baicalin,
wogonoside,
glycyrrhizic
acid)
identified.
Among
these
constituents,
four
components
(glycyrrhizic
acid,
wogonoside)
considered
ingredients.
Second,
further
elucidate
There
was
no
significant
difference
NO
content
administration
group
(dose
converted
into
medium-dose
group)
group.
Then,
contents
acid
determined
method.
This
study
provides
comprehensive
reliable
strategy
quality
identifies
Our
methodology
may
also
be
useful
studying
other
medicines.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 20, 2025
Abstract
The
present
study
aimed
to
investigate
the
effects
of
Trollius
chinensis
Bunge
residues
(TCBR)
on
growth,
meat
quality,
antioxidant
capacity,
intestinal
healthy
and
cecal
microbiota
in
weaned
rabbits.
In
total,
48
30-day-old
rabbits
were
randomly
allocated
into
4
groups,
with
12
replicates
per
group.
Four
diets
formulated
graded
levels
TCBR:
2.0%,
4.0%,
6.0%
represented
as
TCBR2,
TCBR4,
TCBR6
groups
alongside
a
control
group
without
TCBR.
results
showed
that
TCBR2
significantly
alleviated
adverse
clinical
manifestations
improved
survival
rate,
growth
performance,
quality.
while
reducing
feed
conversion
ratio
compared
Mock
also
enhanced
carcass
yield,
semi-eviscerated
liver
index,
increased
jejunal
villus
height
villus/crypt
group,
whereas
no
differences
observed
between
TCBR4
groups.
Notably,
16S
RNA
analysis
revealed
Bacteroidota
elevated
Akkermansia,
Clostridium,
Succiniclasticum
upregulated
Furthermore,
expression
occludin
ZO-1
tissue.
conclusion,
supplementation
performance
attenuated
symptoms
Abstract
Background
The
popularity
of
herbal
formulas
is
increasing
worldwide.
Nevertheless,
the
effective
compound
challenging
to
identify
due
its
intricate
composition
and
multiple
targets.
Methods
An
integration
machine
learning-assisted
approach
was
established,
whereby
particular
action
mechanism
direct
target
were
obtained
through
correlation
compounds,
targets,
metabolites.
association
between
a
an
pathway
selected
from
shortest
path
“compound-target-pathway-disease”
network,
which
analyzed
using
Floyd-Warshall
algorithm.
Subsequently,
investigation
conducted
into
relationship
metabolites
pathways,
as
well
analysis
serum
metabolomic
profiling
selection
metabolite
biomarkers
by
random
forest.
In
order
accurately
acting
most
compound,
compounds
their
targets
investigated
feature-based
prediction
model
AdaBoost.
binding
mode
direct-acting
verified
molecular
docking,
dynamics
simulations,
western
blotting.
this
study,
Baiji
Wuweizi
Granule
(BWG)
employed
elucidate
against
alcoholic
liver
injury
(ALD).
Results
BWG
exerted
influence
on
metabolomic,
resulting
in
identification
seven
potential
biomarkers.
Furthermore,
six
PI3K-AKT
signalling
identified
co-analysis
with
ALD
network.
It
postulated
that
would
bind
key
signaling
pathway,
indicated
compound-target
interaction
(R
2
>
0.95).
dominant
bonding
type
for
hydrogen
bond.
These
results
AKT1
notable
BWG,
2,3,4,7-tetramethoxyphenanthrene
marker
ALD.
present
study
provides
evidence
protective
effect
can
be
mediated
pathway.
Conclusions
Our
findings
demonstrate
value
identifying
underpin
efficacy
formula.
This
foundation
future
clinical
fundamental
research.
ABSTRACT
Celastrol
(CEL)
is
a
natural
pentacyclic
triterpenoid
demonstrating
significant
therapeutic
properties
against
various
diseases.
However,
the
ambiguity
of
target
information
poses
challenge
in
transitioning
CEL
from
traditional
remedy
to
modern
pharmaceutical
agent.
Recently,
emerging
discovery
approaches
products
have
broadened
extensive
avenues
for
uncovering
comprehensive
and
promoting
its
drug
development.
Herein,
diverse
strategies
are
overviewed
pharmacological
toxicological
studies
CEL,
including
chemical
proteomics,
protein
microarray,
degradation‐based
profiling,
proteome‐wide
label‐free
approaches,
network
pharmacology,
target‐based
screening,
multi‐omics
analysis,
hypothesis‐driven
confirmation.
Dozens
targets
been
identified,
which
significantly
suggests
that
functions
as
multi‐target
Further
interaction
analysis
frequency
collected
reveal
PRDXs,
HMGB1,
HSP90,
STAT3,
PKM2
may
serve
key
CEL.
Additionally,
this
review
highlights
positive
role
facilitating
CEL‐based
combination
therapy
delivery,
essential
further
advancing
clinical
applications
Efforts
identification
not
only
aid
unraveling
scientific
underpinnings
multiple
effects
but
also
offer
crucial
insights
development
drugs.
