Molecular network strategies combined with MCnebula2 identify potential active compounds from steamed Polygonatum cyrtonema Hua

Journal of Chromatography A, Год журнала: 2025, Номер 1746, С. 465779 - 465779

Опубликована: Фев. 14, 2025

Язык: Английский

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Physicochemical characterization and protective effects of raw and nine-steamed Polygonatum cyrtonema polysaccharides on cyclophosphamide-induced immunosuppression in mice

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141911 - 141911

Опубликована: Март 1, 2025

Язык: Английский

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Structural characterization of raw and wine-steamed Polygonatum cyrtonema Hua oligosaccharides and their bioactivity on immune regulation via modifying the gut microbiota

International Immunopharmacology, Год журнала: 2025, Номер 153, С. 114468 - 114468

Опубликована: Март 29, 2025

Язык: Английский

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Non-targeted metabolomics-mediated elucidation of metabolite changes in Polygonatum kingianum during traditional steaming with black beans

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Апрель 11, 2025

Introduction Polygonatum is a traditional medicinal and edible plant often prepared by steaming it with ingredients like yellow rice wine black beans. However, the metabolic changes in kingianum (PK) during method beans have not been previously reported. This study aims to explore how addition of degree affect quality PK. Methods quantified polysaccharides, reducing sugars, saponins, flavonoids PK subjected varied cycles, while also integrating color measurement, sensory evaluation, non-targeted metabolomics analysis. Results rich lipids, amino acids, flavonoids, organic acids. Notably, we observed production significant accumulation daidzein daidzin (two primary active compounds derived from beans) as well triterpenoid saponins (including Sandosaponin A, Soyasaponin II-III, V, Pisumsaponin I-II) steamed ( p < 0.05). After three multiple bioactive levels peaked or stabilized, which was consistent (score: 94/100). Compared raw PK, P3 stage exhibited increases total 21.29, 35.02, 49.13, 44.76%, respectively 0.05), sugars showed remarkable increase 160.94-fold 0.01), polysaccharides decreased significantly 43.33% 0.01). Conclusion provides first evidence that can synergistically diversity isoflavones optimal achieved after rounds steaming. These findings validate scientific efficacy processing method. Our results advocate for continued use bean enhance nutritional health-promoting value providing template innovation herbal techniques based on auxiliary ingredients.

Язык: Английский

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Glycan degradation in Polygonati Rhizoma: Effects of traditional ‘nine steaming and nine basking’ on low molecular weight Fructans and polysaccharides

Food Chemistry X, Год журнала: 2024, Номер 25, С. 102131 - 102131

Опубликована: Дек. 31, 2024

Язык: Английский

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Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 31, 2024

Polygonatum cyrtonema Hua (PCH), as an important economic crop, is used raw industrial materials and traditional Chinese medicine. There are significant variations in the quality of PCH from different geographical origins. It can be due to change endophytic fungi soil microbial communities PCH. Therefore, aim this study investigate composition functional prediction main rhizomes explore their impact on medicinal quality. High-throughput sequencing techniques targeting ITS 16S rDNA were employed compare structure biodiversity differences 12 locations Sichuan Guangxi province. Heatmap analysis was for comprehensive statistics visualization richness rhizome all locations. Venn conducted determine total number shared between soil, GraphPad Prism predict related phenotypes at genus level Guangxi. Tax4Fun Fungild metabolic function The results revealed identification 19,387 bacterial amplicon sequence variants (ASVs) 37,990 ASVs with 6,889 ASVs. In addition, 2,948 fungal identified 8,868 1,893 Microbial indicated that mainly composed Ascomycota Basidiomycota , while included Proteobacteria Acidobacteria Bacteroidota Gammatimonadota Firmicutes . Dominant groups such Nitrospira Acidibacter including Mortierella Ceratobasidium Fusarium potential contributors observed traits. top 15 genera, both contain but there abundance. has three unique genera Scleroderma Russula Gliocladiopsis On other hand, Chamaeota correlation microbiota chemical content collecting spots performed by Prism. Burkholderia-Caballeronia-Paraburkholderia Amycolatopsis show inverse proportionality polysaccharides saponins, Enterobacter shows a direct saponins. metabolism pathways positive This provide new insights into mechanisms underlying two major indigenous areas.

Язык: Английский

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Development of inter-retrotransposon amplified polymorphism (IRAP) markers and germplasm DNA fingerprinting of Polygonatum sibiricum: a well-known medicinal species in China

Genetic Resources and Crop Evolution, Год журнала: 2024, Номер unknown

Опубликована: Дек. 31, 2024

Язык: Английский

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