Rapid analysis of the chemical constituents in Qiangli Dingxuan tablets using ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry
Journal of Separation Science,
Год журнала:
2024,
Номер
47(2)
Опубликована: Янв. 1, 2024
Qiangli
Dingxuan
(QLDX)
tablet
is
a
widely
recognized
traditional
Chinese
medicine
formula
that
has
been
extensively
used
in
China
for
decades
to
treat
vertigo,
tinnitus,
and
dizziness
owing
its
outstanding
therapeutic
outcomes.
However,
the
complexity
of
chemical
components
this
makes
it
challenging
separate
identify
these
components.
This
study
presented
an
effective
sensitive
strategy
rapid
separation
simultaneous
structural
identification
QLDX
using
ultra‐performance
liquid
chromatography
with
quadrupole
time‐of‐flight
tandem
mass
spectrometry
UNIFI
platform.
Based
on
retention
times,
accurate
masses,
fragment
ions,
related
literature,
authentic
standards,
119
compounds
were
identified
or
tentatively
characterized;
included
9
iridoids,
12
lignans,
21
phenylpropanoids,
27
flavonoids,
7
phthalides,
43
others.
Among
them,
36
confirmed
reference
standards.
The
representative
various
structures
studied
by
analyzing
their
fragmentation
patterns
characteristic
ions.
In
conclusion,
established
approach
characterizing
constituents
tablet.
proposed
provides
basis
qualitative
analysis
quality
control
manufacturing
process
beneficial
advancing
investigations
into
efficacy
mechanism
action
Язык: Английский
Pharmacological and toxicological effects of Jiangfangbaoxin and determination of its components in the blood of spontaneously hypertensive rats
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 10, 2025
Jiangfangbaoxin
(JFBX)
has
been
used
to
attenuate
essential
hypertension
(EH)
for
over
20
years.
Here,
we
aimed
examine
the
blood
composition
and
toxicological
effects
of
JFBX
using
40
10
male
age-matched
spontaneously
hypertensive
rats
(SHRs)
Wistar-Kyoto,
respectively.
After
12
weeks
intervention,
pressure
left
ventricular
hypertrophy
were
measured.
Subsequently,
their
organs
harvested
pathological
examination.
Ultra-high-performance
liquid
chromate-
graphy
with
quadrupole
time-of-flight
mass
spectrometry
was
explore
components
in
blood.
Low
high-doses
attenuated
both
systolic
diastolic
SHRs.
The
high-
low-dose
groups
showed
significantly
reduced
endothelin-1
increased
nitric
oxide
levels.
myocardial
tissue
levels
tumor
necrosis
factor-α,
interleukin
(IL)-1β,
IL-6
JFBX-high-dose
group
decreased.
Furthermore,
superoxide
dismutase
malonaldehyde
decreased,
results
that
did
not
cause
particularly
severe
side
when
administered
after
weeks.
In
total,
87
precursor
compounds,
including
alkaloids,
iridoid
glycosides,
flavonoids,
siterpenoids,
volatile
components,
organic
phenols/acids,
detected
EH.
No
serious
organ
damage
observed
oral
administration.
Язык: Английский
Simultaneous Determination of 14 Bioactive Components in Fangji Huangqi Tang by UHPLC‐QqQ‐MS Technique
Biomedical Chromatography,
Год журнала:
2025,
Номер
39(5)
Опубликована: Апрель 2, 2025
Fangji
Huangqi
Tang
(FHT)
is
a
traditional
prescription
frequently
utilized
in
clinical
practice,
with
wide
range
of
applications
and
good
therapeutic
effects.
Quality
control
FHT
difficult
because
Chinese
medicine
compounds
usually
contain
vast
array
components
characterized
by
significant
structural
diversity.
A
quick
accurate
method
to
determine
the
content
active
constituents
was
essential,
which
purpose
quality
efficacy
assessment
could
be
achieved.
utilizing
UHPLC-QqQ-MS
technology
multiple
reaction
monitoring
(MRM)
mode
established
quantify
14
bioactive
simultaneously.
These
analytes
included
tetrandrine,
fangchinoline,
calycosin,
calycosin-7-glucoside,
medicarpin,
formononetin,
atractylenolide
I,
II,
III,
liquiritigenin,
isoliquiritigenin,
liquiritin,
isoliquiritin,
glycyrrhizic
acid.
And
our
knowledge,
II
reported
for
first
time
this
paper.
The
thoroughly
validated
stable
reliable
application
regarding
specificity,
linearity,
precision,
stability,
repeatability,
accuracy.
allowed
simultaneous
determination
diverse
structures
trace
amounts
FHT,
ultimately
achieving
its
safe
appropriate
use.
Язык: Английский
Integration of ion mobility-quadrupole time-of-flight mass spectrometry and triple quadrupole-ion trap mass spectrometry coupled with ultra-high performance liquid chromatography for characterizing and quantitatively assay the saponins of Panax quinquefolius flower
Microchemical Journal,
Год журнала:
2024,
Номер
207, С. 112124 - 112124
Опубликована: Ноя. 9, 2024
Язык: Английский
Ultra‐High Performance Liquid Chromatography/Triple Quadrupole Ion‐Trap Mass Spectrometry‐Based Quantitative Assay of 17 Compounds to Unveil the Extraction Difference of Potentially Toxic Components Between the Single Drugs and Sishen Formula
M Wang,
Wandi Hu,
Jia Lou
и другие.
Separation Science Plus,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 2, 2024
ABSTRACT
Sishen
formula
is
a
famous
prescription
utilized
to
treat
kidney
diarrhea.
The
main
components
of
Psoraleae
Fructus
and
Euodiae
are
reported
with
the
liver
toxicity;
however,
dissolution
difference
those
toxic
compounds
among
single
medicine
decoctions
versatile
compatibility
forms
has
yet
not
been
documented
by
quantitative
assays.
We
established
validated
an
ultra‐high
performance
liquid
chromatography/triple
quadrupole‐linear
ion‐trap
mass
spectrometry‐multiple
reaction
monitoring
approach
targeting
17
(e.g.,
four
coumarins
eight
flavonoids
from
Fructus;
three
alkaloids
two
limonoids
Fructus).
Co‐decoction
Myristicae
Semen
or
Schisandrae
Chinensis
could
significantly
affect
Fructus,
whereas
weak
influence
was
observed
for
combining
Fructus.
Combination
increase
contents
both
but
effect
relatively
slight.
Moreover,
in
remarkably
inhibited,
compared
pure
decoction,
variation
between
decoction
formula.
These
results
benefit
bridge
efficacy
toxicity
changes
Язык: Английский