Role of size, surface charge, and PEGylated lipids of lipid nanoparticles (LNPs) on intramuscular delivery of mRNA

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Сен. 11, 2024

Язык: Английский

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Bioinspired cellular membrane-derived vesicles for mRNA delivery

Theranostics, Год журнала: 2024, Номер 14(8), С. 3246 - 3266

Опубликована: Янв. 1, 2024

The rapid advancement of mRNA as vaccines and therapeutic agents in the biomedical field has sparked hope fight against untreatable diseases.Successful clinical application therapeutics largely depends on carriers.Recently, a new exciting focus emerged natural cell-derived vesicles.These nanovesicles offer many functions, including enhanced drug delivery capabilities immune evasion, thereby presenting unique promising platform for effective safe therapeutics.In this study, we summarize characteristics properties biomimetic systems particular, discuss features cellular membrane-derived vesicles (CDVs) combination synthetic with CDVs.

Язык: Английский

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Molecular level design of conjugated polymers based on a synergistic adsorption-photocatalytic strategy for efficient uranium capture

Chemical Engineering Journal, Год журнала: 2024, Номер 496, С. 153493 - 153493

Опубликована: Июнь 27, 2024

Язык: Английский

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Polymer-siRNA nanovectors for treating lung inflammation

Journal of Controlled Release, Год журнала: 2025, Номер 378, С. 1092 - 1102

Опубликована: Янв. 5, 2025

Язык: Английский

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Role of size, surface charge, and PEGylated lipids of lipid nanoparticles (LNPs) on intramuscular delivery of mRNA

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 31, 2024

Background Lipid nanoparticles (LNPs) are currently the most commonly used non-viral gene delivery system. Their physiochemical attributes, encompassing size, charge and surface modifications, significantly affect their behaviors both in vivo vitro. Nevertheless, effects of these properties on transfection distribution LNPs after intramuscular injection remain elusive. In this study, with varying sizes, lipid-based charges PEGylated lipids were formulated to study distribution. Luciferase mRNA (mLuc) was loaded in as a model nucleic acid. Results In vitro results indicated that smaller-sized those neutral potential presented superior efficiency injection. Surprisingly, sizes did not exert notable influence LNPs. Furthermore, shorter acyl chains contributed enhanced due cellular uptake lysosomal escape capabilities. Notably, mechanisms underlying differed among containing various types lipids, which primarily attributed length chain. Conclusions Together, insights underscore pivotal role nanoparticle characteristics route. This only fills crucial knowledge gaps but also provides invaluable directions for effective via

Язык: Английский

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RNA-binding peptide and endosomal escape-assisting peptide (L2) improved siRNA delivery by the hexahistidine–metal assembly

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(40), С. 10309 - 10319

Опубликована: Янв. 1, 2024

The RNA-binding functional peptide L2-NTD is complexed with HmA (siRNA+L2-NTD@HmA), providing efficient siRNA delivery into cells minimal cytotoxicity and degradation.

Язык: Английский

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