Biomimetic Hydrogels from Mixed Gellan Gum and Tryptophan Zipper Self-Assembling Peptides DOI
Riddhesh Doshi,

Dhushanthan Mohanathas,

Md Shariful Islam

и другие.

ACS Macro Letters, Год журнала: 2025, Номер unknown, С. 679 - 686

Опубликована: Май 9, 2025

Peptide self-assembly has been used to fabricate synthetic hydrogels that emulate many of the chemical and physical properties natural hydrogels. However, these materials often lack stability for applications do not display native bioactivity found in tissue. Here we demonstrate a hybrid hydrogel system which self-assembling peptides are integrated with polysaccharides enhance gelation provide improved mechanics bioactivity. A peptide based on tryptophan zipper (trpzip) motif was mixed anionic polysaccharide gellan gum, demonstrating within minutes increased stiffness compared trpzip alone. The material maintained viscoelastic character shear-thinning, self-healing, stress-relaxation order like collagen. All supported cell adhesion viability gum content, promoting assembly into aggregates. enhanced kinetics, stability, make them promising candidates as matrices culture reagents biofabrication syringe extrusion biological delivery.

Язык: Английский

3D bioprinting for bile duct tissue engineering: current status and prospects DOI Creative Commons
Bo Gao

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 14, 2025

Bile duct disorders, including cholangiocarcinoma, primary sclerosing cholangitis, and iatrogenic injuries, pose significant clinical challenges due to limited regenerative capacity the complexity of biliary tree. In recent years, 3D bioprinting has emerged as a promising approach for bile tissue engineering by providing patient-specific geometries facilitating spatial organization cells, scaffolding materials, bioactive factors. This review presents comprehensive overview techniques engineering, focusing on fundamental principles, biomaterial selection, current achievements, key challenges, future perspectives. We systematically discuss latest technological breakthroughs, highlight emerging innovations such organoid-based strategies microfluidic-assisted printing, evaluate prospects translation. Finally, we outline main challenges—such biocompatibility vascularization, immunological barriers, standardization protocols, regulatory hurdles—and propose directions research, emphasizing multidisciplinary collaboration translational studies.

Язык: Английский

Процитировано

0
Biomimetic Hydrogels from Mixed Gellan Gum and Tryptophan Zipper Self-Assembling Peptides DOI
Riddhesh Doshi,

Dhushanthan Mohanathas,

Md Shariful Islam

и другие.

ACS Macro Letters, Год журнала: 2025, Номер unknown, С. 679 - 686

Опубликована: Май 9, 2025

Peptide self-assembly has been used to fabricate synthetic hydrogels that emulate many of the chemical and physical properties natural hydrogels. However, these materials often lack stability for applications do not display native bioactivity found in tissue. Here we demonstrate a hybrid hydrogel system which self-assembling peptides are integrated with polysaccharides enhance gelation provide improved mechanics bioactivity. A peptide based on tryptophan zipper (trpzip) motif was mixed anionic polysaccharide gellan gum, demonstrating within minutes increased stiffness compared trpzip alone. The material maintained viscoelastic character shear-thinning, self-healing, stress-relaxation order like collagen. All supported cell adhesion viability gum content, promoting assembly into aggregates. enhanced kinetics, stability, make them promising candidates as matrices culture reagents biofabrication syringe extrusion biological delivery.

Язык: Английский

Процитировано

0