Triggered “On/off” Luminescent Polypeptide Bowl‐Shaped Nanoparticles for Selective Lighting of Tumor Cells

Small, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Abstract Functional polymeric nanoparticles, especially those with anisotropic structures, have shown significant potential and advantages in biomedical applications including detecting, bioimaging, antimicrobial anticancer. Herein, tetraphenylethylene (TPE) azobenzene modified polypeptides of poly(( L ‐glutamic acid) tetraphenylethylene‐ stat ‐( acid)) (P(GATPE 9 ‐ ‐GA 25 )) azobenzene‐ (P(GAAzo 5 29 ) are synthesized, which self‐assemble into bowl‐shaped nanoparticles (BNPs) controlled diameter, opening size fluorescent property individually, or by co‐assembly. Due to the quenching effect azobenzene, fluorescence coassembled BNPs is completely inhibited. Upon incubated under reduction environment, re‐excited owing break azo bonds. Benefiting from high‐level reductase hypoxic liver cancer cells comparing normal cells, quenched exhibit pronounced signal human hepatoma (HepG2) condition, demonstrating high efficiency reduction‐responsive luminescent for selective screening tumor cells. In addition, it also found that a proper promotes cellular uptake even up micron. Overall, this study provides fresh perspective preparation efficient cell screening.

Язык: Английский

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Microfluidics-Assembled Nanovesicles for Nucleic Acid Delivery

Accounts of Chemical Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

ConspectusMicrofluidic technologies have become a highly effective platform for the precise and reproducible production of nanovesicles used in drug nucleic acid delivery. One their key advantages lies one-step assembly multidrug delivery nanovesicles, which improves batch-to-batch reproducibility by minimizing intermediate steps typically required conventional methods. These often involve complex hydrophobic electrostatic interactions, leading to variability nanovesicle composition performance. Microfluidic systems streamline encapsulation diverse therapeutic agents, including hydrophilic acids, proteins, both small molecules, within single chip, ensuring more consistent process. This capability enables codelivery multiple drugs targeting different disease pathways, is particularly valuable reducing risk resistance.Despite promise delivery, clinical translation has been hindered safety concerns, cytotoxicity, overshadowed efforts improve vivo stability efficiency. Positively charged commonly encapsulate negatively tend exhibit significant cytotoxicity. To address this, charge-shifting materials that respond pH changes or surface modifications proposed as promising strategies. Shifting charge from positive neutral negative at physiological can reduce enhancing feasibility these nanovesicle-based therapies.Microfluidic platforms offer control over properties, particle size, rigidity, morphology, Particle size relatively easy adjust controlling flow rates microfluidic channels, with higher generally producing smaller particles. However, continuous tuning rigidity remains challenging. By manipulation interfacial water layer between amphiphilic components during nanoparticle formation, future designs may achieve greater critical improving cellular uptake biodistribution. While shape using chips not yet fully explored biomedical applications, advances science enable this aspect future, offering further customization properties.The integration modification presents challenges due differing speeds processes. Nanovesicle rapid, whereas modifications, such those involving functional biomolecules, occur slowly require purification steps. Recent advances, rotary valve single-axis camshaft mechanisms, mixing stages process, allowing automation modification, thereby reproducibility.In conclusion, represent approach development multifunctional potential precision medicine. obstacles related scalability, remain, innovations chip design, materials, are paving way broader application settings. Future research, potentially incorporating machine learning, could optimize relationship properties biological outcomes, advancing use

Язык: Английский

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Theranostic Nanocapsules: Heating, Imaging, and Luminescence Nanothermometry

Chemistry of Materials, Год журнала: 2024, Номер 36(7), С. 3285 - 3295

Опубликована: Март 19, 2024

We report a theranostic nanocapsule, working entirely in the near-infrared (NIR) region for photothermal therapy (PTT) with ability to remotely record temperature real time. These nanocapsules are synthesized by one-pot approach, simultaneously coencapsulating bay-substituted dicyclohexylaminoperylene diimide (sPDI) as an optical heater and rationally designed multifunctional LiLuF4:10 mol % Nd3+@LiLuF4 core@shell downshifting NPs (DSNPs), both ratiometric luminescent thermometers imaging probes. Upon laser irradiation, sPDI aggregates convert light heat via nonradiative relaxation, inducing local increase eradicating MCF-7 breast cancer cells. Accurate sensing is achieved situ luminescence of DSNPs. In addition, positive contrast enhancement found computer tomography (CT) images cells under investigation, owing presence Lu3+ DSNPs, demonstrating potential image-guided PTT.

Язык: Английский

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Manipulation of Bowl-Shaped Nanoparticles Self-Assembled from a Bipyridine Pendant Containing Homopolymer

Langmuir, Год журнала: 2024, Номер 40(11), С. 5828 - 5836

Опубликована: Март 8, 2024

The morphological control and transformation of soft nanomaterials are critical for their physical chemical properties, which can be achieved by dynamically regulating the hydrophilicity amphiphilic polymers during self-assembly. Herein, an homopolymer poly(N-(2,2′-bipyridine)-4-acrylamide) (PBPyAA) with bipyridine pendants is synthesized, effect various parameters including initial concentration, temperature, pH, metal ion coordination on self-assembly behavior morphology assemblies investigated. Upon changing concentration PBPyAA, bowl-shaped nanoparticles (BNPs) precisely controlled diameter, opening size, thickness obtained. With decrease pH solution, negatively charged surface BNPs transforms to a positively state. Furthermore, addition divalent ions (Co2+, Mn2+, Zn2+) induces vesicles giant vesicles. above factors essential change PBPyAA dynamically, leading variation local viscosity Overall, manipulation structural achieved, providing fresh insights precise morphologies nanomaterials.

Язык: Английский

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Nonlinear amplification of nano bowl surface concavity on the critical response threshold to biosignals

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 8, 2024

Polymer nanoparticles that can sharply sense and detect biological signals in cells are promising candidates for biomedical theranostic nanomaterials. However, the response ability of current polymer assemblies poorly matches requirement trace concentration level (10

Язык: Английский

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Bowl-shaped alkylated lignin nanoparticles: A novel platform for stable antibacterial Pickering emulsions

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142194 - 142194

Опубликована: Март 1, 2025

Язык: Английский

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Nanoarchitecture tailoring of biomass-derived bowl-shaped carbon superstructures-based LiOH-LiCl composite for low-grade solar heat harvesting

Journal of Colloid and Interface Science, Год журнала: 2025, Номер unknown, С. 137415 - 137415

Опубликована: Март 1, 2025

Язык: Английский

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Preparation and Mechanism Insight of Biodegradable Kippah Vesicles

Small, Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Abstract Kippah vesicles, fully collapsed polymersomes formed during the self‐assembly process, are characterized by a bowl‐shaped nanostructure with large specific surface area, high loading capacity, and an internal void. Current research shows that these structural features have primarily been achieved using non‐biodegradable block copolymers, while fundamental mechanism behind their formation is not well understood. Thus, designing biodegradable kippah vesicles elucidating critical. In this study, tetraphenylethylene (TPE) moiety – luminogen aggregation‐induced emission (AIE) properties strategically introduced into copolymer side chain‐, yielding novel polypeptide poly(ethylene glycol) 45 ‐ ‐poly[(glutamic acid‐TPE) 26 stat ‐(glutamic acid) 29 ] [PEG₄₅‐ b ‐P(GATPE₂₆‐ ‐GA₂₉)]. This could self‐assemble driven hydrophobic interactions hydrogen bonding, as confirmed Fourier‐transform infrared (FTIR) spectroscopy, ultraviolet‐visible (UV–vis) absorption, photoluminescence morphological characterization across different aggregation states. Notably, intrinsic fluorescence of exhibited cellular internalization efficiency excellent cytocompatibility, highlighting potential for biomedical applications such bioimaging targeted delivery.

Язык: Английский

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Revealing into the formation mechanism of various nanostructures self-assembled from a bipyridine containing homopolymer

European Polymer Journal, Год журнала: 2024, Номер 219, С. 113387 - 113387

Опубликована: Авг. 13, 2024

Язык: Английский

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Nonlinear Amplification of Vesicle Membrane Concavity on the Critical Response Threshold to Biosignals

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 7, 2024

Polymer nanoparticles that can sharply sense and detect biological signals in cells are promising candidates for biomedical theranostic nanomaterials. However, the response ability of current polymer assemblies poorly match requirement trace concentration level (10^-6~10^-9 mol/L) cellular biosignals due to their linear signal input-to-function output mode, which impedes practical applications vivo. Here we report a kind bowl-shaped vesicle system with pH-tunable membrane invaginated morphology nonlinearly amplify abilities toward by modulating concavity. Compared conventional spherical nanoparticles, non-spherical concave vesicles reduce critical threshold polymers up 5 orders magnitude, from millimole nanomole level, covering most biosignal windows. Moreover, find this nonlinear gain effect is originated collective impact single on transitioning chain aggregation state individual assemblies, rather than just altering certain unit or chain. This signal-to-response mechanism potential solve tricky problems probing sensing endogenous extremely low physiological concentration.

Язык: Английский

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