Divergent assembly processes? A comparison of the plant and soil microbiome with plant communities in a glacier forefield

FEMS Microbiology Ecology, Год журнала: 2021, Номер 97(10)

Опубликована: Сен. 17, 2021

ABSTRACT Community assembly is a result of dispersal, abiotic and biotic characteristics the habitat as well stochasticity. A direct comparison between microbial ‘macrobial’ organisms hampered by sampling these communities in different studies, at sites or on scales. In glacier forefield Austrian Alps, we recorded soil plant microbiome (bacteria fungi) plants that occurred same landscape close proximity plots. We tested five predictions deduced from processes revealed deviating patterns community types. short, microbes appeared to be less dispersal limited than microbes, strongly responded factors whereas leaf was species specific buffered environmental conditions. The observed differences may attributed organisms’ abilities, exposure habitats airborne propagules characteristics. finding conditional organisms, spatial scale under consideration thus central for our understanding about establishment maintenance biodiversity.

Язык: Английский

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Pollinators mediate floral microbial diversity and microbial network under agrochemical disturbance

Molecular Ecology, Год журнала: 2021, Номер 30(10), С. 2235 - 2247

Опубликована: Март 19, 2021

How pollinators mediate microbiome assembly in the anthosphere is a major unresolved question of theoretical and applied importance face anthropogenic disturbance. We addressed this by linking visitation diverse pollinator functional groups (bees, wasps, flies, butterflies, beetles, true bugs other taxa) to key properties floral (microbial α- β-diversity microbial network) under agrochemical disturbance, using field experiment bactericide fungicide treatments on cultivated strawberries that differ flower abundance. Structural equation modelling was used link disturbance abundance properties. Our results revealed (i) influenced network centrality microbiome, with different affecting properties; (ii) both directly governing source pool microbes indirectly enhancing visitation; (iii) affected primarily fungicide, less so via visitation. These findings improve mechanistic understanding assembly, may be generalizable many plants are visited insect natural managed ecosystems.

Язык: Английский

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New insights into engineered plant-microbe interactions for pesticide removal

Chemosphere, Год журнала: 2022, Номер 309, С. 136635 - 136635

Опубликована: Сен. 29, 2022

Язык: Английский

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A quantitative survey of the blueberry (Vaccinium spp.) culturable nectar microbiome: variation between cultivars, locations, and farm management approaches

FEMS Microbiology Ecology, Год журнала: 2024, Номер 100(3)

Опубликована: Фев. 14, 2024

Microbes in floral nectar can impact both their host plants and visitors, yet little is known about the microbiome of most pollinator-dependent crops. In this study, we examined abundance composition fungi bacteria inhabiting Vaccinium spp. nectar, as well volume sugar concentrations. We compared wild V. myrsinites with two field-grown corymbosum cultivars collected from organic conventional farms. Differences traits microbiomes were identified between but not species. The cultivated also varied greatly farms, whereas management regime had only subtle effects, higher fungal populations detected under management. Nectars hexose-dominant, high cell densities correlated reduced Bacteria more common than blueberry although frequently co-occurred often would be predicted by chance. "Cosmopolitan" microbes that isolated all plants, including Rosenbergiella sp. Symmetrospora symmetrica, identified. This study provides first systematic report microbiome, which may have important implications for pollinator crop health.

Язык: Английский

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A metacommunity ecology approach to understanding microbial community assembly in developing plant seeds

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Июль 22, 2022

Microorganisms have the potential to affect plant seed germination and seedling fitness, ultimately impacting health community dynamics. Because seed-associated microbiota are highly variable across individual plants, species, environments, it is challenging identify dominant processes that underlie assembly, composition, influence of these communities. We propose here metacommunity ecology provides a conceptually useful framework for studying developing seeds, by application principles filtering, species interactions, dispersal at multiple scales. Many studies in microbial already describe assembly pattern-based manner, such as correlating microbiome composition with genotype or tracking diversity metrics treatments limitation experiments. But we see lot opportunities examine understudied aspects microbiology, including trait-based research on mechanisms filtering micro-scale, use pollination exclusion experiments macro-scale studies, an in-depth evaluation how interact

Язык: Английский

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Plant microbiomes as contributors to agricultural terroir

Frontiers in Agronomy, Год журнала: 2023, Номер 5

Опубликована: Окт. 19, 2023

Agricultural products such as tea, chocolate, coffee and wine are valued for their sensorial nutritional qualities. Variation in the growing conditions of a crop can influence plant’s phenotype, thus it behooves agriculturalists to optimize on farms grow highest quality product. The set associated with certain geographic location its product’s chemistry is known terroir. Although terroir plays significant role marketing consumer appreciation well product identity valorization, rarely biochemical differences or factors creating them very understood. word derives from Latin “land”, suggesting simply function geographical where plant grew, while modern usage, understood be result soil type, climate, landscape, topography, biotic interactions agricultural practice. Except fermented food like microbiomes have been little studied contribution crop’s terroir; however, metagenomics metabolomics technologies given scientists tools better observe how microbial diversity impact chemical variation products. Differences inhabiting organs change phytochemistry by altering host metabolism, example increasing nutrients absorbed roots that then deposited leaves, seeds fruits. Plant microbes consume molecules, removing metabolome, they contribute smells flavors own. This review aims synthesize research into rhizosphere, endosphere, phyllosphere, spermosphere, carposphere, anthosphere microbiome influences biochemistry derived products, helping increase beneficial able agriculture improving phytochemical quality.

Язык: Английский

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Are microbes growing on flowers evil? Effects of old flower microbes on fruit set in a wild ginger with one-day flowers, Alpinia japonica (Zingiberaceae)

Metabarcoding and Metagenomics, Год журнала: 2022, Номер 6

Опубликована: Июль 20, 2022

Flowers are colonized and inhabited by diverse microbes. have various mechanisms to suppress microbial growth, such as flower volatiles, reactive oxygen secondary compounds. Besides, plants rapidly replace flowers that a short lifespan, old senesce. They may contribute avoiding adverse effects of the In this study, we investigate if community on impedes fruit seed production in wild ginger with one-day flowers. We focus microbes because they be composed would grow during flowering did not growth. inoculated newly opened microbes, monitored set. also assessed prokaryotic communities using 16S rRNA amplicon sequencing. found six bacterial sequence variants (ASVs) whose proportions were increased These ASVs buds bagged net or paper anthesis, suggesting had been present small numbers prior flowering. Fruit set was negatively associated these ASVs, while not. The results suggest harbor different from those at abundant affect plant reproduction. Although it has received little attention, antagonistic proliferate affected evolution characteristics volatiles life span.

Язык: Английский

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Do flower-colonizing microbes influence floral evolution? A test with fast-cycling Brassica

Journal of Evolutionary Biology, Год журнала: 2024, Номер 37(8), С. 935 - 946

Опубликована: Июнь 21, 2024

Pollinators are thought to be the main drivers of floral evolution. Flowers also colonized by abundant communities microbes that can affect interaction between plants and their pollinators. Very little is known, however, about how flower-colonizing influence Here we performed a 6-generation experimental evolution study using fast-cycling Brassica rapa, in which factorially manipulated presence pollinators flower determine interact driving We measured 6 morphological traits, as well plant mating system attractiveness. Only one traits (flower number) evolved response pollinators, while did not drive any trait, nor they with traits. Moreover, find evidence or affected attractiveness However, found an interactive effect on autonomous selfing, trait expected evolve pollinator limitations. Overall, only weak mediate our ability detect might have been limited pollinator-mediated selection setting. Our results contrast previous (similar) studies, highlighting susceptibility such experiments drift artefacts.

Язык: Английский

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Petal segmentation in CT images based on divide-and-conquer strategy

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июль 15, 2024

Manual segmentation of the petals flower computed tomography (CT) images is time-consuming and labor-intensive because has many petals. In this study, we aim to obtain a three-dimensional (3D) structure Camellia japonica flowers propose petal method using computer vision techniques. Petal on slice fails by simply applying methods shape in CT differs from that objects targeted latest instance methods. To overcome these challenges, crop two-dimensional (2D) long rectangles each image apply segment images. Thanks cropping, it easier cropped We can also use for task number used training augmented cropping. Subsequently, results are integrated into 3D volume data. The experimental show proposed with higher accuracy than without were obtained visualized successfully.

Язык: Английский

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Hyperaccumulation of nickel but not selenium drives floral microbiome differentiation: A study with six species of Brassicaceae

American Journal of Botany, Год журнала: 2024, Номер 111(9)

Опубликована: Авг. 15, 2024

Abstract Premise Intraspecific variation in flower microbiome composition can mediate pollination and reproduction, so understanding the community assembly processes driving this is critical. Yet relative importance of trait‐based host filtering dispersal shaping among‐species floral microbiomes remains unknown. Methods Within two clades Brassicaceae, we compared diversity natural populations focal nickel selenium hyperaccumulator species their non‐accumulating relatives. We assessed strengths elemental composition, plant phylogenetic distance (host filtering), geography (dispersal) composition. Results Species clade had strongly divergent microbiomes, most that driven by followed geographic between and, lastly, distance. Conversely, within clade, divergence was much lower among geography, phylogeny were far weaker determinants variation. Conclusions Our results show strength hyperaccumulation's effect on differs substantially clades, possibly due to elements as selective filters or long‐distance probability different habitats.

Язык: Английский

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