Biosynthetic gene cluster synteny: Orthologous polyketide synthases in Hypogymnia physodes, Hypogymnia tubulosa, and Parmelia sulcata DOI Creative Commons
Nadim Ahmad, Manfred Ritz, Anjuli Calchera

и другие.

MicrobiologyOpen, Год журнала: 2023, Номер 12(5)

Опубликована: Окт. 1, 2023

Lichens are symbiotic associations consisting of a photobiont (algae or cyanobacteria) and mycobiont (fungus), which together generate variety unique secondary metabolites. To access this biosynthetic potential for biotechnological applications, deeper insights into the pathways corresponding gene clusters necessary. Here, we provide comparative view three lichen mycobionts derived from Hypogymnia physodes, tubulosa, Parmelia sulcata. In addition, present high-quality PacBio metagenome sulcata, extracted bin containing 214 clusters. Most in these genomes were associated with T1PKSs, followed by NRPSs terpenes. This study focused on related to polyketide synthesis. Based ketosynthase homology, identified nine highly syntenic all species. Among four belonging nonreducing PKSs, two putatively linked substances orsellinic acid (orcinol depsides depsidones, e.g., lecanoric acid, physodic lobaric acid), one compounds methylated forms (beta orcinol depsides, atranorin), melanins. Five orthologs species reducing PKSs. Our contributes sorting dereplicating vast PKS diversity found lichenized fungi. High-quality sequences common foundation further exploration applications molecular evolution substances.

Язык: Английский

Interactions of PKS gene expression and antimicrobial and antibiofilm activity levels in the lichen-forming fungus Hypogymnia tubulosa due to light and heat stress DOI
Birkan Açıkgöz, Barış Gökalsın, İskender Karaltı

и другие.

Mycological Progress, Год журнала: 2025, Номер 24(1)

Опубликована: Янв. 9, 2025

Язык: Английский

Процитировано

1
Biosynthetic gene cluster synteny: Orthologous polyketide synthases in Hypogymnia physodes, Hypogymnia tubulosa, and Parmelia sulcata DOI Creative Commons
Nadim Ahmad, Manfred Ritz, Anjuli Calchera

и другие.

MicrobiologyOpen, Год журнала: 2023, Номер 12(5)

Опубликована: Окт. 1, 2023

Lichens are symbiotic associations consisting of a photobiont (algae or cyanobacteria) and mycobiont (fungus), which together generate variety unique secondary metabolites. To access this biosynthetic potential for biotechnological applications, deeper insights into the pathways corresponding gene clusters necessary. Here, we provide comparative view three lichen mycobionts derived from Hypogymnia physodes, tubulosa, Parmelia sulcata. In addition, present high-quality PacBio metagenome sulcata, extracted bin containing 214 clusters. Most in these genomes were associated with T1PKSs, followed by NRPSs terpenes. This study focused on related to polyketide synthesis. Based ketosynthase homology, identified nine highly syntenic all species. Among four belonging nonreducing PKSs, two putatively linked substances orsellinic acid (orcinol depsides depsidones, e.g., lecanoric acid, physodic lobaric acid), one compounds methylated forms (beta orcinol depsides, atranorin), melanins. Five orthologs species reducing PKSs. Our contributes sorting dereplicating vast PKS diversity found lichenized fungi. High-quality sequences common foundation further exploration applications molecular evolution substances.

Язык: Английский

Процитировано

2